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Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura.

Lin X, Zhang Q, Wu Z, Du Y - PLoS ONE (2015)

Bottom Line: It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea.Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs.The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.

ABSTRACT
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.

No MeSH data available.


Related in: MedlinePlus

Phylogenic analysis of SlituOR3 and homologues.Neighbor-Joining method was used. Shown here is the optimal tree with the sum of branch length = 6.00509761. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown also. Poisson correction method was used to calculate the evolutionary distances. CSPR stands for the candidate sex pheromone receptor. SlituOR18 was used as outgroup. Slitu: Spodoptera litura (OR3:AEY84943.2; OR6:AGI96748.1;OR16:AGI96751.1;OR11: AGI96749.1;OR13:ACL81181.1;OR18:AGA16498.1); Slitt: Spodoptera littoralis (OR6: ACL81183.1;OR16: ACL81182.1;OR11:ACL81180.1;OR13: AGI96750.1) Se: Spodoptera exigua (OR6:AGH58119.1;OR16:AGH58122.1;OR11:AGH58120.1;OR13:AGH58121.1); Si: Sesamia inferens (OR: AGY14579.2); Har: Helicoverpa armigera (OR6: AGK90000.1;OR3:ACS45306.1;OR:AIG51863.1;OR2: ACS45305.1;OR11:ACF32965.1;OR13:ACJ12370.1);Has: Helicoverpa assulta (OR6:AGK90014.1;OR3:ACS45309.1;OR14:AHI44516.1;OR11:AJD81549.1;OR2:ACS45308.1;OR13:AJD81551.1); As: Agrotis segetum (OR10:AGS41449.1;OR7:AGS41446.1;OR1:AGS41441.1;OR6:AGS41445.1;OR8:AGS41447.1;OR9:AGS41448.1;OR5:AGS41444.1;OR3:AGS41442.1;OR4:AGS41443.1);Ms:Mythimna separate(OR1: BAG71414.1);Bmo:Bombyx mori (OR3:NP_001036925.1;OR4:BAH57981.1;OR1:NP_001036875.1);Bma: Bombyx mandarina(OR3:ACT34882.1); Px:Plutella xylostella(OR1:AGK43824.1); Ape: Antheraea pernyi(OR1: CBH19583.1); Opa:Ostrinia palustralis(OR: BAH57978.1);On: Ostrinia nubilalis(OR: BAJ61929.1;OR5:ADB89182.1;OR4:ADB89181.1); Oza:Ostrinia zaguliaevi(OR: BAH57976.1); Ol:Ostrinia latipennis(OR:BAH57981.1); Oo:Ostrinia ovalipennis(OR:BAH57979.1);Di:Diaphania indica(OR1: BAG71417.1); Of: Ostrinia furnacalis(OR:AGG91642.1;OR4:AFK30397.1;OR3:BAR43446.1;OR7:BAR43449.1);Cpo:Cydia pomonella(OR3: AFC91713.2);Apo: Antheraea polyphemus(OR1: CBH19582.1).
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pone.0131407.g004: Phylogenic analysis of SlituOR3 and homologues.Neighbor-Joining method was used. Shown here is the optimal tree with the sum of branch length = 6.00509761. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown also. Poisson correction method was used to calculate the evolutionary distances. CSPR stands for the candidate sex pheromone receptor. SlituOR18 was used as outgroup. Slitu: Spodoptera litura (OR3:AEY84943.2; OR6:AGI96748.1;OR16:AGI96751.1;OR11: AGI96749.1;OR13:ACL81181.1;OR18:AGA16498.1); Slitt: Spodoptera littoralis (OR6: ACL81183.1;OR16: ACL81182.1;OR11:ACL81180.1;OR13: AGI96750.1) Se: Spodoptera exigua (OR6:AGH58119.1;OR16:AGH58122.1;OR11:AGH58120.1;OR13:AGH58121.1); Si: Sesamia inferens (OR: AGY14579.2); Har: Helicoverpa armigera (OR6: AGK90000.1;OR3:ACS45306.1;OR:AIG51863.1;OR2: ACS45305.1;OR11:ACF32965.1;OR13:ACJ12370.1);Has: Helicoverpa assulta (OR6:AGK90014.1;OR3:ACS45309.1;OR14:AHI44516.1;OR11:AJD81549.1;OR2:ACS45308.1;OR13:AJD81551.1); As: Agrotis segetum (OR10:AGS41449.1;OR7:AGS41446.1;OR1:AGS41441.1;OR6:AGS41445.1;OR8:AGS41447.1;OR9:AGS41448.1;OR5:AGS41444.1;OR3:AGS41442.1;OR4:AGS41443.1);Ms:Mythimna separate(OR1: BAG71414.1);Bmo:Bombyx mori (OR3:NP_001036925.1;OR4:BAH57981.1;OR1:NP_001036875.1);Bma: Bombyx mandarina(OR3:ACT34882.1); Px:Plutella xylostella(OR1:AGK43824.1); Ape: Antheraea pernyi(OR1: CBH19583.1); Opa:Ostrinia palustralis(OR: BAH57978.1);On: Ostrinia nubilalis(OR: BAJ61929.1;OR5:ADB89182.1;OR4:ADB89181.1); Oza:Ostrinia zaguliaevi(OR: BAH57976.1); Ol:Ostrinia latipennis(OR:BAH57981.1); Oo:Ostrinia ovalipennis(OR:BAH57979.1);Di:Diaphania indica(OR1: BAG71417.1); Of: Ostrinia furnacalis(OR:AGG91642.1;OR4:AFK30397.1;OR3:BAR43446.1;OR7:BAR43449.1);Cpo:Cydia pomonella(OR3: AFC91713.2);Apo: Antheraea polyphemus(OR1: CBH19582.1).

Mentions: Phylogenetic analysis showed that SlituOR3 clustered with the ORs containing most closely sex pheromone receptor S. littoralis OR6 [44] and is related closely to male-specific receptor H. virescens OR16[45]. And there were so many other male-specific receptors or sex pheromone receptors closely in the cluster: for example, H. virescens OR14 [45], H. virescens OR15 [45], M.separate OR1[46], M.sexta OR1[47], H. virescens OR11 [45] and so on. We named this cluster the ‘candidate sex pheromone receptor subfamily (Fig 4).


Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura.

Lin X, Zhang Q, Wu Z, Du Y - PLoS ONE (2015)

Phylogenic analysis of SlituOR3 and homologues.Neighbor-Joining method was used. Shown here is the optimal tree with the sum of branch length = 6.00509761. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown also. Poisson correction method was used to calculate the evolutionary distances. CSPR stands for the candidate sex pheromone receptor. SlituOR18 was used as outgroup. Slitu: Spodoptera litura (OR3:AEY84943.2; OR6:AGI96748.1;OR16:AGI96751.1;OR11: AGI96749.1;OR13:ACL81181.1;OR18:AGA16498.1); Slitt: Spodoptera littoralis (OR6: ACL81183.1;OR16: ACL81182.1;OR11:ACL81180.1;OR13: AGI96750.1) Se: Spodoptera exigua (OR6:AGH58119.1;OR16:AGH58122.1;OR11:AGH58120.1;OR13:AGH58121.1); Si: Sesamia inferens (OR: AGY14579.2); Har: Helicoverpa armigera (OR6: AGK90000.1;OR3:ACS45306.1;OR:AIG51863.1;OR2: ACS45305.1;OR11:ACF32965.1;OR13:ACJ12370.1);Has: Helicoverpa assulta (OR6:AGK90014.1;OR3:ACS45309.1;OR14:AHI44516.1;OR11:AJD81549.1;OR2:ACS45308.1;OR13:AJD81551.1); As: Agrotis segetum (OR10:AGS41449.1;OR7:AGS41446.1;OR1:AGS41441.1;OR6:AGS41445.1;OR8:AGS41447.1;OR9:AGS41448.1;OR5:AGS41444.1;OR3:AGS41442.1;OR4:AGS41443.1);Ms:Mythimna separate(OR1: BAG71414.1);Bmo:Bombyx mori (OR3:NP_001036925.1;OR4:BAH57981.1;OR1:NP_001036875.1);Bma: Bombyx mandarina(OR3:ACT34882.1); Px:Plutella xylostella(OR1:AGK43824.1); Ape: Antheraea pernyi(OR1: CBH19583.1); Opa:Ostrinia palustralis(OR: BAH57978.1);On: Ostrinia nubilalis(OR: BAJ61929.1;OR5:ADB89182.1;OR4:ADB89181.1); Oza:Ostrinia zaguliaevi(OR: BAH57976.1); Ol:Ostrinia latipennis(OR:BAH57981.1); Oo:Ostrinia ovalipennis(OR:BAH57979.1);Di:Diaphania indica(OR1: BAG71417.1); Of: Ostrinia furnacalis(OR:AGG91642.1;OR4:AFK30397.1;OR3:BAR43446.1;OR7:BAR43449.1);Cpo:Cydia pomonella(OR3: AFC91713.2);Apo: Antheraea polyphemus(OR1: CBH19582.1).
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Related In: Results  -  Collection

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pone.0131407.g004: Phylogenic analysis of SlituOR3 and homologues.Neighbor-Joining method was used. Shown here is the optimal tree with the sum of branch length = 6.00509761. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown also. Poisson correction method was used to calculate the evolutionary distances. CSPR stands for the candidate sex pheromone receptor. SlituOR18 was used as outgroup. Slitu: Spodoptera litura (OR3:AEY84943.2; OR6:AGI96748.1;OR16:AGI96751.1;OR11: AGI96749.1;OR13:ACL81181.1;OR18:AGA16498.1); Slitt: Spodoptera littoralis (OR6: ACL81183.1;OR16: ACL81182.1;OR11:ACL81180.1;OR13: AGI96750.1) Se: Spodoptera exigua (OR6:AGH58119.1;OR16:AGH58122.1;OR11:AGH58120.1;OR13:AGH58121.1); Si: Sesamia inferens (OR: AGY14579.2); Har: Helicoverpa armigera (OR6: AGK90000.1;OR3:ACS45306.1;OR:AIG51863.1;OR2: ACS45305.1;OR11:ACF32965.1;OR13:ACJ12370.1);Has: Helicoverpa assulta (OR6:AGK90014.1;OR3:ACS45309.1;OR14:AHI44516.1;OR11:AJD81549.1;OR2:ACS45308.1;OR13:AJD81551.1); As: Agrotis segetum (OR10:AGS41449.1;OR7:AGS41446.1;OR1:AGS41441.1;OR6:AGS41445.1;OR8:AGS41447.1;OR9:AGS41448.1;OR5:AGS41444.1;OR3:AGS41442.1;OR4:AGS41443.1);Ms:Mythimna separate(OR1: BAG71414.1);Bmo:Bombyx mori (OR3:NP_001036925.1;OR4:BAH57981.1;OR1:NP_001036875.1);Bma: Bombyx mandarina(OR3:ACT34882.1); Px:Plutella xylostella(OR1:AGK43824.1); Ape: Antheraea pernyi(OR1: CBH19583.1); Opa:Ostrinia palustralis(OR: BAH57978.1);On: Ostrinia nubilalis(OR: BAJ61929.1;OR5:ADB89182.1;OR4:ADB89181.1); Oza:Ostrinia zaguliaevi(OR: BAH57976.1); Ol:Ostrinia latipennis(OR:BAH57981.1); Oo:Ostrinia ovalipennis(OR:BAH57979.1);Di:Diaphania indica(OR1: BAG71417.1); Of: Ostrinia furnacalis(OR:AGG91642.1;OR4:AFK30397.1;OR3:BAR43446.1;OR7:BAR43449.1);Cpo:Cydia pomonella(OR3: AFC91713.2);Apo: Antheraea polyphemus(OR1: CBH19582.1).
Mentions: Phylogenetic analysis showed that SlituOR3 clustered with the ORs containing most closely sex pheromone receptor S. littoralis OR6 [44] and is related closely to male-specific receptor H. virescens OR16[45]. And there were so many other male-specific receptors or sex pheromone receptors closely in the cluster: for example, H. virescens OR14 [45], H. virescens OR15 [45], M.separate OR1[46], M.sexta OR1[47], H. virescens OR11 [45] and so on. We named this cluster the ‘candidate sex pheromone receptor subfamily (Fig 4).

Bottom Line: It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea.Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs.The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.

ABSTRACT
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.

No MeSH data available.


Related in: MedlinePlus