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Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura.

Lin X, Zhang Q, Wu Z, Du Y - PLoS ONE (2015)

Bottom Line: It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea.Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs.The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.

ABSTRACT
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.

No MeSH data available.


Related in: MedlinePlus

Alignment of amino acid sequences of SlituOR3 with six verified moth sex pheromone receptors.Gaps are indicated with slash (-). Identical amino acids are marked in the bottom with *. Transmembrane domains identified with MEMSAT3 & MEMSAT-SVM are underlined and numbered I to VII. GI numbers of each OR are: BmorOR1(GI:112983558); BmorOR3 (GI:112982950); HvirOR13(GI:51127338); MsepOR(GI:226001155), OzeaOR(GI:284010026); PxylOR1(GI:205361602); SlituOR3(GI: 381211953).
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pone.0131407.g003: Alignment of amino acid sequences of SlituOR3 with six verified moth sex pheromone receptors.Gaps are indicated with slash (-). Identical amino acids are marked in the bottom with *. Transmembrane domains identified with MEMSAT3 & MEMSAT-SVM are underlined and numbered I to VII. GI numbers of each OR are: BmorOR1(GI:112983558); BmorOR3 (GI:112982950); HvirOR13(GI:51127338); MsepOR(GI:226001155), OzeaOR(GI:284010026); PxylOR1(GI:205361602); SlituOR3(GI: 381211953).

Mentions: Distinct and separate bands were obtained for SlituOR3 by RT-PCR of antennal cDNA and it was of the expected sizes given the primer design. The SlituOR3 full-length cDNA sequence was 1574 bp, encoding 432 amino acids (Fig 1). Predicted by the Phobius and MANSAT3, the SlituOR3 has seven transmembrane domains (Fig 2). Aligning the SlituOR3 with verified moth pheromone receptors indicated a high degree of conservation across species (Fig 3). SlituOR3 annotations have been submitted to GenBank and accession numbers is JN835270.


Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura.

Lin X, Zhang Q, Wu Z, Du Y - PLoS ONE (2015)

Alignment of amino acid sequences of SlituOR3 with six verified moth sex pheromone receptors.Gaps are indicated with slash (-). Identical amino acids are marked in the bottom with *. Transmembrane domains identified with MEMSAT3 & MEMSAT-SVM are underlined and numbered I to VII. GI numbers of each OR are: BmorOR1(GI:112983558); BmorOR3 (GI:112982950); HvirOR13(GI:51127338); MsepOR(GI:226001155), OzeaOR(GI:284010026); PxylOR1(GI:205361602); SlituOR3(GI: 381211953).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488349&req=5

pone.0131407.g003: Alignment of amino acid sequences of SlituOR3 with six verified moth sex pheromone receptors.Gaps are indicated with slash (-). Identical amino acids are marked in the bottom with *. Transmembrane domains identified with MEMSAT3 & MEMSAT-SVM are underlined and numbered I to VII. GI numbers of each OR are: BmorOR1(GI:112983558); BmorOR3 (GI:112982950); HvirOR13(GI:51127338); MsepOR(GI:226001155), OzeaOR(GI:284010026); PxylOR1(GI:205361602); SlituOR3(GI: 381211953).
Mentions: Distinct and separate bands were obtained for SlituOR3 by RT-PCR of antennal cDNA and it was of the expected sizes given the primer design. The SlituOR3 full-length cDNA sequence was 1574 bp, encoding 432 amino acids (Fig 1). Predicted by the Phobius and MANSAT3, the SlituOR3 has seven transmembrane domains (Fig 2). Aligning the SlituOR3 with verified moth pheromone receptors indicated a high degree of conservation across species (Fig 3). SlituOR3 annotations have been submitted to GenBank and accession numbers is JN835270.

Bottom Line: It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea.Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs.The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.

ABSTRACT
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.

No MeSH data available.


Related in: MedlinePlus