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Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi.

Liu Y, Chen M, Su J, Ma H, Zheng X, Li Q, Shi S, Qin L - PLoS ONE (2015)

Bottom Line: This gene does not contain introns.ApmVg decreased dramatically during embryonic development.These results represent the first study of mVg outside M. sexta and B. mori and provide insight into the physiological role and evolution of mVgs.

View Article: PubMed Central - PubMed

Affiliation: Department of Sericulture, Shenyang Agricultural University, Shenyang, Liaoning, China; Sericultural Institute of Liaoning Province, Fengcheng, Liaoning, China.

ABSTRACT
Microvitellogenin (mVg) is a relatively small vitellogenic protein only characterized in the eggs of the lepidopteran insects Manduca sexta and Bombyx mori. In the present study, we report a novel mVg (ApmVg) isolated from the Chinese oak silkworm Antheraea pernyi. The obtained ApmVg cDNA sequence contains an open reading frame of 783 bp encoding a protein of 260 amino acids with a predicted molecular weight of 29.96 kDa. This gene does not contain introns. Structural analysis revealed that this protein shares putative conserved domains with the lepidopteran low-molecular weight lipoprotein, which belongs to the lipoprotein_11 superfamily. The protein sequence of ApmVg exhibits 48% sequence identity with mVg from M. sexta and 40-47% sequence identity with the 30K lipoproteins from B. mori. Phylogenetic analysis suggests that ApmVg is a novel member of the lepidopteran low-molecular weight lipoproteins. Transcriptional analysis indicated that ApmVg mRNA is mainly expressed in the fat body (both female and male) during post-diapause development of the pupal stage, and it was also detected in ovaries and spermaries in smaller amounts. RT-PCR and Western blot analyses revealed that ApmVg is synthesized by the fat body and secreted into hemolymph and ultimately accumulates in eggs. The ApmVg transcript can be detected in the fat bodies of female pupae four days after treatment with 20-hydroxyecdysone and shows an expression pattern distinct from that of vitellogenin (Vg), which is detectable throughout diapausing and in post-diapause development. ApmVg decreased dramatically during embryonic development. These results represent the first study of mVg outside M. sexta and B. mori and provide insight into the physiological role and evolution of mVgs.

No MeSH data available.


Phylogenetic tree based on amino acid sequence comparisons.The numbers near the branch represent bootstrap percentages. The topology was tested using bootstrap analyses (1,000 replicates). The GenBank accession number is shown following the organism names.
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pone.0131751.g002: Phylogenetic tree based on amino acid sequence comparisons.The numbers near the branch represent bootstrap percentages. The topology was tested using bootstrap analyses (1,000 replicates). The GenBank accession number is shown following the organism names.

Mentions: To deduce the phylogenetic relationship, a total of 13 protein sequences were considered, including A. pernyi mVg and related proteins (Fig 2). Homologous proteins from A. mylitta and S. cynthia ricini were not included, because they had incomplete protein sequences. In the ML phylogenetic tree, mVg from A. pernyi was most closely related to the mVg-like protein from A. assama with a 100% bootstrapping value; mVg from M. sexta was the next most closely related.


Identification and Characterization of a Novel Microvitellogenin from the Chinese Oak Silkworm Antheraea pernyi.

Liu Y, Chen M, Su J, Ma H, Zheng X, Li Q, Shi S, Qin L - PLoS ONE (2015)

Phylogenetic tree based on amino acid sequence comparisons.The numbers near the branch represent bootstrap percentages. The topology was tested using bootstrap analyses (1,000 replicates). The GenBank accession number is shown following the organism names.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488348&req=5

pone.0131751.g002: Phylogenetic tree based on amino acid sequence comparisons.The numbers near the branch represent bootstrap percentages. The topology was tested using bootstrap analyses (1,000 replicates). The GenBank accession number is shown following the organism names.
Mentions: To deduce the phylogenetic relationship, a total of 13 protein sequences were considered, including A. pernyi mVg and related proteins (Fig 2). Homologous proteins from A. mylitta and S. cynthia ricini were not included, because they had incomplete protein sequences. In the ML phylogenetic tree, mVg from A. pernyi was most closely related to the mVg-like protein from A. assama with a 100% bootstrapping value; mVg from M. sexta was the next most closely related.

Bottom Line: This gene does not contain introns.ApmVg decreased dramatically during embryonic development.These results represent the first study of mVg outside M. sexta and B. mori and provide insight into the physiological role and evolution of mVgs.

View Article: PubMed Central - PubMed

Affiliation: Department of Sericulture, Shenyang Agricultural University, Shenyang, Liaoning, China; Sericultural Institute of Liaoning Province, Fengcheng, Liaoning, China.

ABSTRACT
Microvitellogenin (mVg) is a relatively small vitellogenic protein only characterized in the eggs of the lepidopteran insects Manduca sexta and Bombyx mori. In the present study, we report a novel mVg (ApmVg) isolated from the Chinese oak silkworm Antheraea pernyi. The obtained ApmVg cDNA sequence contains an open reading frame of 783 bp encoding a protein of 260 amino acids with a predicted molecular weight of 29.96 kDa. This gene does not contain introns. Structural analysis revealed that this protein shares putative conserved domains with the lepidopteran low-molecular weight lipoprotein, which belongs to the lipoprotein_11 superfamily. The protein sequence of ApmVg exhibits 48% sequence identity with mVg from M. sexta and 40-47% sequence identity with the 30K lipoproteins from B. mori. Phylogenetic analysis suggests that ApmVg is a novel member of the lepidopteran low-molecular weight lipoproteins. Transcriptional analysis indicated that ApmVg mRNA is mainly expressed in the fat body (both female and male) during post-diapause development of the pupal stage, and it was also detected in ovaries and spermaries in smaller amounts. RT-PCR and Western blot analyses revealed that ApmVg is synthesized by the fat body and secreted into hemolymph and ultimately accumulates in eggs. The ApmVg transcript can be detected in the fat bodies of female pupae four days after treatment with 20-hydroxyecdysone and shows an expression pattern distinct from that of vitellogenin (Vg), which is detectable throughout diapausing and in post-diapause development. ApmVg decreased dramatically during embryonic development. These results represent the first study of mVg outside M. sexta and B. mori and provide insight into the physiological role and evolution of mVgs.

No MeSH data available.