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Enhanced Bone Tissue Regeneration by Porous Gelatin Composites Loaded with the Chinese Herbal Decoction Danggui Buxue Tang.

Wang WL, Sheu SY, Chen YS, Kao ST, Fu YT, Kuo TF, Chen KY, Yao CH - PLoS ONE (2015)

Bottom Line: Danggui Buxue Tang (DBT) is a traditional Chinese herbal decoction containing Radix Astragali and Radix Angelicae sinensis.IVIS images revealed a stronger fluorescent signal in GGTDBT-treated defect than in GGT-treated defect at 8 weeks after implantation.Micro-CT analysis demonstrated that the level of repair from week 4 to 8 increased from 42.1% to 71.2% at the sites treated with GGTDBT, while that increased from 33.2% to 54.1% at GGT-treated sites.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan.

ABSTRACT
Danggui Buxue Tang (DBT) is a traditional Chinese herbal decoction containing Radix Astragali and Radix Angelicae sinensis. Pharmacological results indicate that DBT can stimulate bone cell proliferation and differentiation. The aim of the study was to investigate the efficacy of adding DBT to bone substitutes on bone regeneration following bone injury. DBT was incorporated into porous composites (GGT) made from genipin-crosslinked gelatin and β-triclacium phosphates as bone substitutes (GGTDBT). The biological response of mouse calvarial bone to these composites was evaluated by in vivo imaging systems (IVIS), micro-computed tomography (micro-CT), and histology analysis. IVIS images revealed a stronger fluorescent signal in GGTDBT-treated defect than in GGT-treated defect at 8 weeks after implantation. Micro-CT analysis demonstrated that the level of repair from week 4 to 8 increased from 42.1% to 71.2% at the sites treated with GGTDBT, while that increased from 33.2% to 54.1% at GGT-treated sites. These findings suggest that the GGTDBT stimulates the innate regenerative capacity of bone, supporting their use in bone tissue regeneration.

No MeSH data available.


Related in: MedlinePlus

Biocompatibility of composites.(A) Macroscopic observations of tissue-covered implants, after GGT and GGTDBT composites were implanted subcutaneously at 8 weeks post-surgery. (B) Tissue reaction after GGT and GGTDBT composites were implanted for 4 and 8 weeks.
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pone.0131999.g001: Biocompatibility of composites.(A) Macroscopic observations of tissue-covered implants, after GGT and GGTDBT composites were implanted subcutaneously at 8 weeks post-surgery. (B) Tissue reaction after GGT and GGTDBT composites were implanted for 4 and 8 weeks.

Mentions: No complication, such as wound infection, inflammation, festering, hematoma, or disturbed wound healing at the surgical sites, was found for any of the rats throughout the experiments, as shown in Fig 1A. The histological changes in tissue specimens were examined by the H&E staining at 4 and 8 weeks post-surgery (Fig 1B). The GGT and GGTDBT composites did not result in histopathological changes, indicating that these composites exhibited favorable biocompatibility.


Enhanced Bone Tissue Regeneration by Porous Gelatin Composites Loaded with the Chinese Herbal Decoction Danggui Buxue Tang.

Wang WL, Sheu SY, Chen YS, Kao ST, Fu YT, Kuo TF, Chen KY, Yao CH - PLoS ONE (2015)

Biocompatibility of composites.(A) Macroscopic observations of tissue-covered implants, after GGT and GGTDBT composites were implanted subcutaneously at 8 weeks post-surgery. (B) Tissue reaction after GGT and GGTDBT composites were implanted for 4 and 8 weeks.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488343&req=5

pone.0131999.g001: Biocompatibility of composites.(A) Macroscopic observations of tissue-covered implants, after GGT and GGTDBT composites were implanted subcutaneously at 8 weeks post-surgery. (B) Tissue reaction after GGT and GGTDBT composites were implanted for 4 and 8 weeks.
Mentions: No complication, such as wound infection, inflammation, festering, hematoma, or disturbed wound healing at the surgical sites, was found for any of the rats throughout the experiments, as shown in Fig 1A. The histological changes in tissue specimens were examined by the H&E staining at 4 and 8 weeks post-surgery (Fig 1B). The GGT and GGTDBT composites did not result in histopathological changes, indicating that these composites exhibited favorable biocompatibility.

Bottom Line: Danggui Buxue Tang (DBT) is a traditional Chinese herbal decoction containing Radix Astragali and Radix Angelicae sinensis.IVIS images revealed a stronger fluorescent signal in GGTDBT-treated defect than in GGT-treated defect at 8 weeks after implantation.Micro-CT analysis demonstrated that the level of repair from week 4 to 8 increased from 42.1% to 71.2% at the sites treated with GGTDBT, while that increased from 33.2% to 54.1% at GGT-treated sites.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan.

ABSTRACT
Danggui Buxue Tang (DBT) is a traditional Chinese herbal decoction containing Radix Astragali and Radix Angelicae sinensis. Pharmacological results indicate that DBT can stimulate bone cell proliferation and differentiation. The aim of the study was to investigate the efficacy of adding DBT to bone substitutes on bone regeneration following bone injury. DBT was incorporated into porous composites (GGT) made from genipin-crosslinked gelatin and β-triclacium phosphates as bone substitutes (GGTDBT). The biological response of mouse calvarial bone to these composites was evaluated by in vivo imaging systems (IVIS), micro-computed tomography (micro-CT), and histology analysis. IVIS images revealed a stronger fluorescent signal in GGTDBT-treated defect than in GGT-treated defect at 8 weeks after implantation. Micro-CT analysis demonstrated that the level of repair from week 4 to 8 increased from 42.1% to 71.2% at the sites treated with GGTDBT, while that increased from 33.2% to 54.1% at GGT-treated sites. These findings suggest that the GGTDBT stimulates the innate regenerative capacity of bone, supporting their use in bone tissue regeneration.

No MeSH data available.


Related in: MedlinePlus