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Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure.

Ilavenil S, Park HS, Vijayakumar M, Arasu MV, Kim da H, Ravikumar S, Choi KC - ScientificWorldJournal (2015)

Bottom Line: Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids.Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract.In conclusion, novel L. plantarum KCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.

View Article: PubMed Central - PubMed

Affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.

ABSTRACT
The aim of the study was to isolate and characterize the lactic acid bacteria (LAB) from animal manure. Among the thirty LAB strains, four strains, namely, KCC-25, KCC-26, KCC-27, and KCC-28, showed good cell growth and antifungal activity and were selected for further characterization. Biochemical and physiology properties of strains confirmed that the strains are related to the Lactobacillus sp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towards Lactobacillus plantarum. The strains exhibited susceptibility against commonly used antibiotics with negative hemolytic property. Strains KCC-25, KCC-26, KCC-27, and KCC-28 showed strong antifungal activity against Aspergillus fumigatus, Penicillium chrysogenum, Penicillium roqueforti, Botrytis elliptica, and Fusarium oxysporum, respectively. Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids. Further, the production of extracellular proteolytic and glycolytic enzymes, survival under low pH, bile salts, and gastric juice together with positive bile salt hydrolase (Bsh) activity, cholesterol lowering, cell surface hydrophobicity, and aggregation properties were the strains advantages. Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract. In conclusion, novel L. plantarum KCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.

No MeSH data available.


Bile salt tolerant properties of L. plantarum. (a) KCC-25, KCC-26, KCC-27, and KCC-28 growth in sodium deoxycholate (DCA). (b) KCC-25, KCC-26, KCC-27, and KCC-28 growth in oxgall. abcdDifferent letters within treatment represent the significant difference (p < 0.05).
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fig4: Bile salt tolerant properties of L. plantarum. (a) KCC-25, KCC-26, KCC-27, and KCC-28 growth in sodium deoxycholate (DCA). (b) KCC-25, KCC-26, KCC-27, and KCC-28 growth in oxgall. abcdDifferent letters within treatment represent the significant difference (p < 0.05).

Mentions: Low pH tolerance property of KCC-25, KCC-26, KCC-27, and KCC-28 was investigated by culturing KCC-25, KCC-26, KCC-27, and KCC-28 at different pH (2 and 3). The marginal reduction of the bacterial growth was observed at pH-2 and pH-3. It indicated that these strains had the ability to grow in low pH (Figures 3(a)-3(b)). KCC-25, KCC-26, KCC-27, and KCC-28 were supplemented with 0.3% oxgall and 0.5% sodium deoxycholate for 3 h showed a marginal decrease of % of bacterial viability as compared with control (Figures 4(a)-4(b)). In addition, the KCC-25, KCC-26, KCC-27, and KCC-28 exposed to the artificial gastric juice of pH-2 and pH-3 with pepsin for 3 h reduced the % of bacterial viability as compared with control bacteria. However, a significant % of KCC-25, KCC-26, KCC-27, and KCC-28 were surviving in the harsh gastric juice conditions (Figure 5(a)). All these strains had significant ability to survive in the low pH, bile salts, and gastric juice. These strains had the ability to utilize the cholesterol from MRS broth after 24 h incubation (Figure 5(b)). The maximum level of cholesterol was assimilated by KCC-26 as compared with other strains. The Lactobacillus sp. KCC-25, KCC-26, KCC-27, and KCC-28 produced different kind of extracellular enzymes such as β-Galactosidase, α-Galactosidase, α-Glucosidase, β-Glucosidase, leucine arylamidase, valine arylamidase, acid phosphatase, naphthol-AS-biphosphohydrolase, trypsin-like serine protease, and other enzymes (Table 4). All the strains showed significant hydrophobicity and aggregations property (Table 5).


Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure.

Ilavenil S, Park HS, Vijayakumar M, Arasu MV, Kim da H, Ravikumar S, Choi KC - ScientificWorldJournal (2015)

Bile salt tolerant properties of L. plantarum. (a) KCC-25, KCC-26, KCC-27, and KCC-28 growth in sodium deoxycholate (DCA). (b) KCC-25, KCC-26, KCC-27, and KCC-28 growth in oxgall. abcdDifferent letters within treatment represent the significant difference (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488156&req=5

fig4: Bile salt tolerant properties of L. plantarum. (a) KCC-25, KCC-26, KCC-27, and KCC-28 growth in sodium deoxycholate (DCA). (b) KCC-25, KCC-26, KCC-27, and KCC-28 growth in oxgall. abcdDifferent letters within treatment represent the significant difference (p < 0.05).
Mentions: Low pH tolerance property of KCC-25, KCC-26, KCC-27, and KCC-28 was investigated by culturing KCC-25, KCC-26, KCC-27, and KCC-28 at different pH (2 and 3). The marginal reduction of the bacterial growth was observed at pH-2 and pH-3. It indicated that these strains had the ability to grow in low pH (Figures 3(a)-3(b)). KCC-25, KCC-26, KCC-27, and KCC-28 were supplemented with 0.3% oxgall and 0.5% sodium deoxycholate for 3 h showed a marginal decrease of % of bacterial viability as compared with control (Figures 4(a)-4(b)). In addition, the KCC-25, KCC-26, KCC-27, and KCC-28 exposed to the artificial gastric juice of pH-2 and pH-3 with pepsin for 3 h reduced the % of bacterial viability as compared with control bacteria. However, a significant % of KCC-25, KCC-26, KCC-27, and KCC-28 were surviving in the harsh gastric juice conditions (Figure 5(a)). All these strains had significant ability to survive in the low pH, bile salts, and gastric juice. These strains had the ability to utilize the cholesterol from MRS broth after 24 h incubation (Figure 5(b)). The maximum level of cholesterol was assimilated by KCC-26 as compared with other strains. The Lactobacillus sp. KCC-25, KCC-26, KCC-27, and KCC-28 produced different kind of extracellular enzymes such as β-Galactosidase, α-Galactosidase, α-Glucosidase, β-Glucosidase, leucine arylamidase, valine arylamidase, acid phosphatase, naphthol-AS-biphosphohydrolase, trypsin-like serine protease, and other enzymes (Table 4). All the strains showed significant hydrophobicity and aggregations property (Table 5).

Bottom Line: Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids.Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract.In conclusion, novel L. plantarum KCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.

View Article: PubMed Central - PubMed

Affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.

ABSTRACT
The aim of the study was to isolate and characterize the lactic acid bacteria (LAB) from animal manure. Among the thirty LAB strains, four strains, namely, KCC-25, KCC-26, KCC-27, and KCC-28, showed good cell growth and antifungal activity and were selected for further characterization. Biochemical and physiology properties of strains confirmed that the strains are related to the Lactobacillus sp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towards Lactobacillus plantarum. The strains exhibited susceptibility against commonly used antibiotics with negative hemolytic property. Strains KCC-25, KCC-26, KCC-27, and KCC-28 showed strong antifungal activity against Aspergillus fumigatus, Penicillium chrysogenum, Penicillium roqueforti, Botrytis elliptica, and Fusarium oxysporum, respectively. Fermentation studies noted that the strains were able to produce significant amount of lactic, acetic, and succinic acids. Further, the production of extracellular proteolytic and glycolytic enzymes, survival under low pH, bile salts, and gastric juice together with positive bile salt hydrolase (Bsh) activity, cholesterol lowering, cell surface hydrophobicity, and aggregation properties were the strains advantages. Thus, KCC-25, KCC-26, KCC-27, and KCC-28 could have the survival ability in the harsh condition of the digestive system in the gastrointestinal tract. In conclusion, novel L. plantarum KCC-25, KCC-26, KCC-27, and KCC-28 could be considered as potential antimicrobial probiotic strains.

No MeSH data available.