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In vitro and in vivo antileishmanial effects of aloe-emodin on Leishmania major.

Dalimi A, Delavari M, Ghaffarifar F, Sadraei J - J Tradit Complement Med (2015)

Bottom Line: Therefore, finding a safe and effective treatment is of great importance.Topical application of aloe-emodin ointment likewise reduced the ulcer size.No significant differences in biochemical analysis were observed between the control and treated groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT
Cutaneous leishmaniasis is a common parasitic disease that is endemic in some parts of Iran. The drugs of choice used for leishmaniasis therapy are associated with a risk of recurrence and serious adverse effects. Therefore, finding a safe and effective treatment is of great importance. In the present study, the effect of aloe-emodin on the growth of Leishmania major amastigotes was evaluated under in vitro conditions. In addition, the efficacy of a topical of aloe-emodin ointment was investigated in BALB/c mice with cutaneous leishmanial ulcers. Different concentrations (40 μg/mL, 80 μg/mL, 120 μg/mL, and 160 μg/mL) of aloe-emodin were tested on Leishmania amastigotes twice: 24 hours and 48 hours. The induced apoptosis and necrotic effects of two concentrations (40 μg/mL and 120 μg/mL) of aloe-emodin on promastigotes were investigated by flow cytometry. Under the in vivo condition, aloe-emodin ointment efficacy was evaluated at two concentrations (i.e., 0.1% and 1%). Serum indicator factors of the test and control groups were tested to evaluate the toxic effects of this compound on the liver and kidney. Results showed that aloe-emodin inhibited the growth of Leishmania amastigotes and induced apoptosis in promastigotes. Topical application of aloe-emodin ointment likewise reduced the ulcer size. No significant differences in biochemical analysis were observed between the control and treated groups. In conclusion, aloe-emodin showed antileishmanial effects under in vitro and in vivo conditions and may be used in clinical trials.

No MeSH data available.


Related in: MedlinePlus

Flow cytometry result. Promastigotes were stained with Annexin V and propidium iodide 48 hours after treatment with aloe-emodin.
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fig3: Flow cytometry result. Promastigotes were stained with Annexin V and propidium iodide 48 hours after treatment with aloe-emodin.

Mentions: The treatment of promastigotes at two concentrations of aloe-emodin (i.e., 40 μg/mL and 120 μg/mL) for 24 hours and 48 hours resulted in necrotic and apoptotic effects in the parasite. The percent of apoptosis in promastigotes induced by 40 μg/mL and 120 μg/mL of aloe-emodin after 24 hours was 13.45% and 68.3%, respectively; after 48 hours, it was 15.43% and 70.2%, respectively (Fig. 3).


In vitro and in vivo antileishmanial effects of aloe-emodin on Leishmania major.

Dalimi A, Delavari M, Ghaffarifar F, Sadraei J - J Tradit Complement Med (2015)

Flow cytometry result. Promastigotes were stained with Annexin V and propidium iodide 48 hours after treatment with aloe-emodin.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488107&req=5

fig3: Flow cytometry result. Promastigotes were stained with Annexin V and propidium iodide 48 hours after treatment with aloe-emodin.
Mentions: The treatment of promastigotes at two concentrations of aloe-emodin (i.e., 40 μg/mL and 120 μg/mL) for 24 hours and 48 hours resulted in necrotic and apoptotic effects in the parasite. The percent of apoptosis in promastigotes induced by 40 μg/mL and 120 μg/mL of aloe-emodin after 24 hours was 13.45% and 68.3%, respectively; after 48 hours, it was 15.43% and 70.2%, respectively (Fig. 3).

Bottom Line: Therefore, finding a safe and effective treatment is of great importance.Topical application of aloe-emodin ointment likewise reduced the ulcer size.No significant differences in biochemical analysis were observed between the control and treated groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT
Cutaneous leishmaniasis is a common parasitic disease that is endemic in some parts of Iran. The drugs of choice used for leishmaniasis therapy are associated with a risk of recurrence and serious adverse effects. Therefore, finding a safe and effective treatment is of great importance. In the present study, the effect of aloe-emodin on the growth of Leishmania major amastigotes was evaluated under in vitro conditions. In addition, the efficacy of a topical of aloe-emodin ointment was investigated in BALB/c mice with cutaneous leishmanial ulcers. Different concentrations (40 μg/mL, 80 μg/mL, 120 μg/mL, and 160 μg/mL) of aloe-emodin were tested on Leishmania amastigotes twice: 24 hours and 48 hours. The induced apoptosis and necrotic effects of two concentrations (40 μg/mL and 120 μg/mL) of aloe-emodin on promastigotes were investigated by flow cytometry. Under the in vivo condition, aloe-emodin ointment efficacy was evaluated at two concentrations (i.e., 0.1% and 1%). Serum indicator factors of the test and control groups were tested to evaluate the toxic effects of this compound on the liver and kidney. Results showed that aloe-emodin inhibited the growth of Leishmania amastigotes and induced apoptosis in promastigotes. Topical application of aloe-emodin ointment likewise reduced the ulcer size. No significant differences in biochemical analysis were observed between the control and treated groups. In conclusion, aloe-emodin showed antileishmanial effects under in vitro and in vivo conditions and may be used in clinical trials.

No MeSH data available.


Related in: MedlinePlus