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Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits.

Ostvar O, Shadvar S, Yahaghi E, Azma K, Fayyaz AF, Ahmadi K, Nowrouzian I - Diagn Pathol (2015)

Bottom Line: Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group.A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21.This results indicated that PRP treated group' enhanced angiogenesis at the wound beds as compared to no treatment group.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. omidostovar@yahoo.com.

ABSTRACT

Background: Platelet-rich plasma (PRP) contains numerous growth factors to promote wound healing and angiogenesis. The aim of this study was to gain further information about the benefits of platelet-rich-plasma for healing cutaneous acute to chronic wounds.

Methods: A total of 30 New Zealand albino rabbits (n = 15/group) were randomly assigned to two experimental groups: control group, and PRP group. Bilateral resection defects measuring 3 cm were surgically created on the dorsolateral of the cutaneous in animals and the defects were randomly divided into two mentioned groups. Wound area, neovascularization, size and epithelialization were compared on days 7, 14 and 21 post-wounding. Histopathological analyses were conducted on 15 specimens from each group after sacrifice by the cellular aspects of the regeneration of the tissue.

Results: Our results were indicated that the wound area of PRP was smaller than that in the non-treated group on days 7, 14 and 21. Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group. A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21. This results indicated that PRP treated group' enhanced angiogenesis at the wound beds as compared to no treatment group.

Conclusion: These results could be useful for researchers in the growing fields of tissue repair and experimental wound healing. Further studies will be essential to determine the role of PRP in clinical practice.

No MeSH data available.


Related in: MedlinePlus

Cutaneous lesions of PRP rabbit after 1 week: the lesion is humid and non to mild -exudative, with marginal epithelization starting from the lesion edges, the wound is <100 % epithelialized, with marked fibrin deposition. There is moderate erythema of the peri and intra-wound area and firm
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Fig1: Cutaneous lesions of PRP rabbit after 1 week: the lesion is humid and non to mild -exudative, with marginal epithelization starting from the lesion edges, the wound is <100 % epithelialized, with marked fibrin deposition. There is moderate erythema of the peri and intra-wound area and firm

Mentions: The rabbits were randomly allocated into two groups, each group containing fifteen submitted animals. Two symmetrical wounds were created on either side of the back of each rabbit that were made in the laterodorsal cutaneous. It is worth noting that one wound was as control group and PRP was applied on wound in PRP group (Fig. 1). Both wounds were then covered with a polyurethane film, and evaluated macroscopically and histologically on days 7, 14 and 21 after the operation.Fig 1


Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits.

Ostvar O, Shadvar S, Yahaghi E, Azma K, Fayyaz AF, Ahmadi K, Nowrouzian I - Diagn Pathol (2015)

Cutaneous lesions of PRP rabbit after 1 week: the lesion is humid and non to mild -exudative, with marginal epithelization starting from the lesion edges, the wound is <100 % epithelialized, with marked fibrin deposition. There is moderate erythema of the peri and intra-wound area and firm
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4487960&req=5

Fig1: Cutaneous lesions of PRP rabbit after 1 week: the lesion is humid and non to mild -exudative, with marginal epithelization starting from the lesion edges, the wound is <100 % epithelialized, with marked fibrin deposition. There is moderate erythema of the peri and intra-wound area and firm
Mentions: The rabbits were randomly allocated into two groups, each group containing fifteen submitted animals. Two symmetrical wounds were created on either side of the back of each rabbit that were made in the laterodorsal cutaneous. It is worth noting that one wound was as control group and PRP was applied on wound in PRP group (Fig. 1). Both wounds were then covered with a polyurethane film, and evaluated macroscopically and histologically on days 7, 14 and 21 after the operation.Fig 1

Bottom Line: Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group.A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21.This results indicated that PRP treated group' enhanced angiogenesis at the wound beds as compared to no treatment group.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. omidostovar@yahoo.com.

ABSTRACT

Background: Platelet-rich plasma (PRP) contains numerous growth factors to promote wound healing and angiogenesis. The aim of this study was to gain further information about the benefits of platelet-rich-plasma for healing cutaneous acute to chronic wounds.

Methods: A total of 30 New Zealand albino rabbits (n = 15/group) were randomly assigned to two experimental groups: control group, and PRP group. Bilateral resection defects measuring 3 cm were surgically created on the dorsolateral of the cutaneous in animals and the defects were randomly divided into two mentioned groups. Wound area, neovascularization, size and epithelialization were compared on days 7, 14 and 21 post-wounding. Histopathological analyses were conducted on 15 specimens from each group after sacrifice by the cellular aspects of the regeneration of the tissue.

Results: Our results were indicated that the wound area of PRP was smaller than that in the non-treated group on days 7, 14 and 21. Furthermore, a significant decrease of the wound size was observed in PRP groups that were significantly greater than that in the control group. A significant increase of the mean vascular density was noted in the PRP treated groups compared to the control groups at day 14 and especially day 21. This results indicated that PRP treated group' enhanced angiogenesis at the wound beds as compared to no treatment group.

Conclusion: These results could be useful for researchers in the growing fields of tissue repair and experimental wound healing. Further studies will be essential to determine the role of PRP in clinical practice.

No MeSH data available.


Related in: MedlinePlus