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Cellular imaging of endosome entrapped small gold nanoparticles.

Kim CS, Li X, Jiang Y, Yan B, Tonga GY, Ray M, Solfiell DJ, Rotello VM - MethodsX (2015)

Bottom Line: However, the current detection method (i.e., transmission electron microscopy) for such sAuNPs is limited due to the extensive sample preparation and the limited field of view.Here we use confocal laser scanning microscopy to provide endosome-entrapped sAuNP distributions and to quantify particle uptake into cells.The quantitative capabilities of the system were confirmed by inductively coupled plasma-mass spectrometry, with an observed linear relation between scattering intensity and the initial cellular uptake of sAuNPs using 4 nm and 6 nm core particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA.

ABSTRACT
Small gold nanoparticles (sAuNPs, <10 nm in a core diameter) have been used for drug delivery and cancer therapy due to their high payload to carrier ratio. Information about the amount and location of sAuNPs in cells and tissues is critical to many applications. However, the current detection method (i.e., transmission electron microscopy) for such sAuNPs is limited due to the extensive sample preparation and the limited field of view. Here we use confocal laser scanning microscopy to provide endosome-entrapped sAuNP distributions and to quantify particle uptake into cells. The quantitative capabilities of the system were confirmed by inductively coupled plasma-mass spectrometry, with an observed linear relation between scattering intensity and the initial cellular uptake of sAuNPs using 4 nm and 6 nm core particles. The summary of the method is: •This non-invasive imaging strategy provides a tool for label-free real-time tracking and quantification of sAuNPs using a commercially available confocal laser scanning microscope.•Scattering intensity depends on particle size.•The linear relation established between scattering intensity and uptaken gold amount enables simultaneous quantitative assessment through simple image analysis.

No MeSH data available.


Ligand structure of AuNPs used in this study, and dynamic light scattering analysis and zeta-potential measurements of particles in PB and cell culture media.
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fig0005: Ligand structure of AuNPs used in this study, and dynamic light scattering analysis and zeta-potential measurements of particles in PB and cell culture media.

Mentions: Gold nanoparticles with different core sizes (2, 4, and 6 nm in diameter) were chosen to determine the size requirements for optical detection of endosome-entrapped sAuNPs. All sAuNPs were synthesized bearing the same surface functionality (Figs. 1, S1–S3, and Supporting information for detailed synthesis).


Cellular imaging of endosome entrapped small gold nanoparticles.

Kim CS, Li X, Jiang Y, Yan B, Tonga GY, Ray M, Solfiell DJ, Rotello VM - MethodsX (2015)

Ligand structure of AuNPs used in this study, and dynamic light scattering analysis and zeta-potential measurements of particles in PB and cell culture media.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487928&req=5

fig0005: Ligand structure of AuNPs used in this study, and dynamic light scattering analysis and zeta-potential measurements of particles in PB and cell culture media.
Mentions: Gold nanoparticles with different core sizes (2, 4, and 6 nm in diameter) were chosen to determine the size requirements for optical detection of endosome-entrapped sAuNPs. All sAuNPs were synthesized bearing the same surface functionality (Figs. 1, S1–S3, and Supporting information for detailed synthesis).

Bottom Line: However, the current detection method (i.e., transmission electron microscopy) for such sAuNPs is limited due to the extensive sample preparation and the limited field of view.Here we use confocal laser scanning microscopy to provide endosome-entrapped sAuNP distributions and to quantify particle uptake into cells.The quantitative capabilities of the system were confirmed by inductively coupled plasma-mass spectrometry, with an observed linear relation between scattering intensity and the initial cellular uptake of sAuNPs using 4 nm and 6 nm core particles.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA 01003, USA.

ABSTRACT
Small gold nanoparticles (sAuNPs, <10 nm in a core diameter) have been used for drug delivery and cancer therapy due to their high payload to carrier ratio. Information about the amount and location of sAuNPs in cells and tissues is critical to many applications. However, the current detection method (i.e., transmission electron microscopy) for such sAuNPs is limited due to the extensive sample preparation and the limited field of view. Here we use confocal laser scanning microscopy to provide endosome-entrapped sAuNP distributions and to quantify particle uptake into cells. The quantitative capabilities of the system were confirmed by inductively coupled plasma-mass spectrometry, with an observed linear relation between scattering intensity and the initial cellular uptake of sAuNPs using 4 nm and 6 nm core particles. The summary of the method is: •This non-invasive imaging strategy provides a tool for label-free real-time tracking and quantification of sAuNPs using a commercially available confocal laser scanning microscope.•Scattering intensity depends on particle size.•The linear relation established between scattering intensity and uptaken gold amount enables simultaneous quantitative assessment through simple image analysis.

No MeSH data available.