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Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA).

Hall SA, Stucke D, Morrone B, Lebelt D, Zanella AJ - MethodsX (2015)

Bottom Line: Until recently, simultaneous quantification of cytokine profiles had not been possible.Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample.The following pro-inflammatory and anti-inflammatory cytokines were quantified in equine plasma and serum samples: interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α. •The objective of this study was to quantify six equine cytokines simultaneously using the BioPlex(®) 200 system in equine EDTA-plasma and serum.•It demonstrates an increased number of detectable cytokines over published studies.•This technology has the advantage of reduced sample volume and assay time compared to traditional sandwich ELISAs.

View Article: PubMed Central - PubMed

Affiliation: Scotland's Rural College, Edinburgh, Scotland, United Kingdom.

ABSTRACT
Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti-inflammatory cytokines were quantified in equine plasma and serum samples: interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α. •The objective of this study was to quantify six equine cytokines simultaneously using the BioPlex(®) 200 system in equine EDTA-plasma and serum.•It demonstrates an increased number of detectable cytokines over published studies.•This technology has the advantage of reduced sample volume and assay time compared to traditional sandwich ELISAs.

No MeSH data available.


Related in: MedlinePlus

Standard curves for each cytokine comparing single-plex and six-plex. A) IL-2, B) IL-4, C) IL-6, D) IL-10, E) IFN-γ, F) TNF-α.
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fig0015: Standard curves for each cytokine comparing single-plex and six-plex. A) IL-2, B) IL-4, C) IL-6, D) IL-10, E) IFN-γ, F) TNF-α.

Mentions: Standard curves were similar for single-plex and multiplex assays, with multiplex assays showing increased fluorescence for IL-4 and IL-6, however the reason for this is unclear (Fig. 3).


Simultaneous detection and quantification of six equine cytokines in plasma using a fluorescent microsphere immunoassay (FMIA).

Hall SA, Stucke D, Morrone B, Lebelt D, Zanella AJ - MethodsX (2015)

Standard curves for each cytokine comparing single-plex and six-plex. A) IL-2, B) IL-4, C) IL-6, D) IL-10, E) IFN-γ, F) TNF-α.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487921&req=5

fig0015: Standard curves for each cytokine comparing single-plex and six-plex. A) IL-2, B) IL-4, C) IL-6, D) IL-10, E) IFN-γ, F) TNF-α.
Mentions: Standard curves were similar for single-plex and multiplex assays, with multiplex assays showing increased fluorescence for IL-4 and IL-6, however the reason for this is unclear (Fig. 3).

Bottom Line: Until recently, simultaneous quantification of cytokine profiles had not been possible.Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample.The following pro-inflammatory and anti-inflammatory cytokines were quantified in equine plasma and serum samples: interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α. •The objective of this study was to quantify six equine cytokines simultaneously using the BioPlex(®) 200 system in equine EDTA-plasma and serum.•It demonstrates an increased number of detectable cytokines over published studies.•This technology has the advantage of reduced sample volume and assay time compared to traditional sandwich ELISAs.

View Article: PubMed Central - PubMed

Affiliation: Scotland's Rural College, Edinburgh, Scotland, United Kingdom.

ABSTRACT
Cytokines are cell signalling proteins that mediate a number of different physiological responses. They are also biomarkers for inflammatory conditions and potential diagnostic references for diseases. Until recently, simultaneous quantification of cytokine profiles had not been possible. Now however, fluorescent microsphere immunoassays (FMIA) are able to measure multiple cytokines in a single sample. The following pro-inflammatory and anti-inflammatory cytokines were quantified in equine plasma and serum samples: interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α. •The objective of this study was to quantify six equine cytokines simultaneously using the BioPlex(®) 200 system in equine EDTA-plasma and serum.•It demonstrates an increased number of detectable cytokines over published studies.•This technology has the advantage of reduced sample volume and assay time compared to traditional sandwich ELISAs.

No MeSH data available.


Related in: MedlinePlus