Limits...
Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin.

Igaz I, Nyírő G, Nagy Z, Butz H, Nagy Z, Perge P, Sahin P, Tóth M, Rácz K, Igaz P, Patócs A - Int J Endocrinol (2015)

Bottom Line: The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown.Results.The biological relevance of hsa-miR-27a modulation by hormones is unclear, but the responsiveness of circulating microRNAs to hormones of the pituitary-adrenal axis is noteworthy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Szent Imre Teaching Hospital, Tétényi Street 12-16, Budapest 1115, Hungary.

ABSTRACT
Purpose. The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown. We have studied the effects of dexamethasone and adrenocorticotropin on the expression of five circulating microRNAs (hsa-miR-27a, hsa-miR-200b, hsa-miR-214, hsa-miR-483-5p, and hsa-miR-503) reported to be related to the adrenal cortex in plasma samples. Methods. Expression of microRNAs was studied in plasma samples of 10 individuals examined by 1 mg dexamethasone suppression test and another 10 individuals stimulated by 250 μg tetracosactide (adrenocorticotropin). Total RNA was isolated and microRNA expression was analyzed by real-time reverse transcription quantitative polymerase chain reaction normalized to cel-miR-39 as reference. Results. Only circulating hsa-miR-27a proved to be significantly modulated in vivo by hormonal treatments: its expression was upregulated by dexamethasone whereas it was suppressed by adrenocorticotropin. Secreted hsa-miR-27a was significantly induced by dexamethasone in vitro in NCI-H295R cells, as well. The expression of hsa-miR-483-5p proposed as diagnostic marker for adrenocortical malignancy was not affected by dexamethasone or tetracosactide administration. Conclusions. hsa-miR-27a expression is modulated by hormones of the hypothalamic-pituitary-adrenal axis both in vitro and in vivo. The biological relevance of hsa-miR-27a modulation by hormones is unclear, but the responsiveness of circulating microRNAs to hormones of the pituitary-adrenal axis is noteworthy.

No MeSH data available.


Related in: MedlinePlus

Expression change of secreted hsa-miR-27a after 100 nM dexamethasone treatment for 8 hours in the NCI-H295R adrenocortical cell line mediums normalized to cel-mir-39. ΔCt values are represented: increased ΔCt indicates lower expression, whereas decreased ΔCt indicates higher expression (mean ± SD). ∗p < 0.05, n = 4. Results of t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4487905&req=5

fig3: Expression change of secreted hsa-miR-27a after 100 nM dexamethasone treatment for 8 hours in the NCI-H295R adrenocortical cell line mediums normalized to cel-mir-39. ΔCt values are represented: increased ΔCt indicates lower expression, whereas decreased ΔCt indicates higher expression (mean ± SD). ∗p < 0.05, n = 4. Results of t-test.

Mentions: To confirm the dexamethasone responsiveness of hsa-miR-27a, we have performed in vitro experiments on the adrenocortical NCI-H295R cell line. We have observed dexamethasone responsiveness in vitro, as well. Dexamethasone significantly induced secreted hsa-miR-27a expression in NCI-H295R culture medium (Figure 3). Dexamethasone induced intracellular hsa-miR-27a in NCI-H295R cells too, but this has not reached statistical significance (data not shown). These results demonstrate that hsa-miR-27a is secreted by NCI-H295R cells and the level of secreted hsa-miR-27a is induced by dexamethasone, as well.


Analysis of Circulating MicroRNAs In Vivo following Administration of Dexamethasone and Adrenocorticotropin.

Igaz I, Nyírő G, Nagy Z, Butz H, Nagy Z, Perge P, Sahin P, Tóth M, Rácz K, Igaz P, Patócs A - Int J Endocrinol (2015)

Expression change of secreted hsa-miR-27a after 100 nM dexamethasone treatment for 8 hours in the NCI-H295R adrenocortical cell line mediums normalized to cel-mir-39. ΔCt values are represented: increased ΔCt indicates lower expression, whereas decreased ΔCt indicates higher expression (mean ± SD). ∗p < 0.05, n = 4. Results of t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487905&req=5

fig3: Expression change of secreted hsa-miR-27a after 100 nM dexamethasone treatment for 8 hours in the NCI-H295R adrenocortical cell line mediums normalized to cel-mir-39. ΔCt values are represented: increased ΔCt indicates lower expression, whereas decreased ΔCt indicates higher expression (mean ± SD). ∗p < 0.05, n = 4. Results of t-test.
Mentions: To confirm the dexamethasone responsiveness of hsa-miR-27a, we have performed in vitro experiments on the adrenocortical NCI-H295R cell line. We have observed dexamethasone responsiveness in vitro, as well. Dexamethasone significantly induced secreted hsa-miR-27a expression in NCI-H295R culture medium (Figure 3). Dexamethasone induced intracellular hsa-miR-27a in NCI-H295R cells too, but this has not reached statistical significance (data not shown). These results demonstrate that hsa-miR-27a is secreted by NCI-H295R cells and the level of secreted hsa-miR-27a is induced by dexamethasone, as well.

Bottom Line: The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown.Results.The biological relevance of hsa-miR-27a modulation by hormones is unclear, but the responsiveness of circulating microRNAs to hormones of the pituitary-adrenal axis is noteworthy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Szent Imre Teaching Hospital, Tétényi Street 12-16, Budapest 1115, Hungary.

ABSTRACT
Purpose. The interaction of hormones of the pituitary-adrenal axis and adrenal cortex-associated circulating microRNAs is mostly unknown. We have studied the effects of dexamethasone and adrenocorticotropin on the expression of five circulating microRNAs (hsa-miR-27a, hsa-miR-200b, hsa-miR-214, hsa-miR-483-5p, and hsa-miR-503) reported to be related to the adrenal cortex in plasma samples. Methods. Expression of microRNAs was studied in plasma samples of 10 individuals examined by 1 mg dexamethasone suppression test and another 10 individuals stimulated by 250 μg tetracosactide (adrenocorticotropin). Total RNA was isolated and microRNA expression was analyzed by real-time reverse transcription quantitative polymerase chain reaction normalized to cel-miR-39 as reference. Results. Only circulating hsa-miR-27a proved to be significantly modulated in vivo by hormonal treatments: its expression was upregulated by dexamethasone whereas it was suppressed by adrenocorticotropin. Secreted hsa-miR-27a was significantly induced by dexamethasone in vitro in NCI-H295R cells, as well. The expression of hsa-miR-483-5p proposed as diagnostic marker for adrenocortical malignancy was not affected by dexamethasone or tetracosactide administration. Conclusions. hsa-miR-27a expression is modulated by hormones of the hypothalamic-pituitary-adrenal axis both in vitro and in vivo. The biological relevance of hsa-miR-27a modulation by hormones is unclear, but the responsiveness of circulating microRNAs to hormones of the pituitary-adrenal axis is noteworthy.

No MeSH data available.


Related in: MedlinePlus