Limits...
Preventive Treatment with Ketamine Attenuates the Ischaemia-Reperfusion Response in a Chronic Postischaemia Pain Model.

Liman S, Cheung CW, Wong KL, Tai W, Qiu Q, Ng KF, Choi SW, Irwin M - Oxid Med Cell Longev (2015)

Bottom Line: Ketamine treated rats showed a significantly lower temperature in the ischaemic hindpaw compared to saline (P < 0.01) and methylprednisolone (P < 0.05) groups.Mechanical and cold allodynia were significantly lower in the ischaemic side in the ketamine group (P < 0.05).In conclusion, immediate administration of ketamine after an ischaemia-reperfusion injury can alleviate pain and inflammation in the CPIP model and has potential to treat postischaemic pain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anaesthesiology, The University of Hong Kong, Pokfulam, Hong Kong.

ABSTRACT
Ischemia and inflammation may be pathophysiological mechanisms of complex regional pain syndrome (CRPS). Ketamine has proposed anti-inflammatory effects and has been used for treating CRPS. This study aimed to evaluate anti-inflammatory and analgesic effects of ketamine after ischaemia-reperfusion injury in a chronic postischaemia pain (CPIP) model of CRPS-I. Using this model, ischemia was induced in the hindlimbs of male Sprague-Dawley rats. Ketamine, methylprednisolone, or saline was administered immediately after reperfusion. Physical effects, (oedema, temperature, and mechanical and cold allodynia) in the bilateral hindpaws, were assessed from 48 hours after reperfusion. Fewer (56%) rats in the ketamine group developed CPIP at the 48th hour after reperfusion (nonsignificant). Ketamine treated rats showed a significantly lower temperature in the ischaemic hindpaw compared to saline (P < 0.01) and methylprednisolone (P < 0.05) groups. Mechanical and cold allodynia were significantly lower in the ischaemic side in the ketamine group (P < 0.05). Proinflammatory cytokines TNF-α and IL-2 were significantly lower at the 48th hour after reperfusion in ketamine and methylprednisolone groups, compared to saline (all P < 0.05). In conclusion, immediate administration of ketamine after an ischaemia-reperfusion injury can alleviate pain and inflammation in the CPIP model and has potential to treat postischaemic pain.

No MeSH data available.


Related in: MedlinePlus

Cold allodynia of KE, MP, and NS groups on the ipsilateral (a) and contralateral side (b) from baseline before ischaemia until 7 days after reperfusion. On the ipsilateral side, the withdrawal threshold to acetone of group KE was significantly higher, compared with groups NS (P < 0.05) and MP (P < 0.05). On the contralateral side, the withdrawal threshold to acetone was significantly higher in KE (P < 0.001) and MP (P < 0.001) groups than that of NS; *P < 0.05; ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4487903&req=5

fig4: Cold allodynia of KE, MP, and NS groups on the ipsilateral (a) and contralateral side (b) from baseline before ischaemia until 7 days after reperfusion. On the ipsilateral side, the withdrawal threshold to acetone of group KE was significantly higher, compared with groups NS (P < 0.05) and MP (P < 0.05). On the contralateral side, the withdrawal threshold to acetone was significantly higher in KE (P < 0.001) and MP (P < 0.001) groups than that of NS; *P < 0.05; ***P < 0.001.

Mentions: Ketamine significantly alleviated both mechanical and cold allodynia in the ipsilateral hindpaw. On the ipsilateral side, the withdrawal threshold to the von Frey fibre in group KE was significantly higher than that in groups NS and MP (P < 0.01 and P < 0.05, resp., Figure 3(a)). On the contralateral sides, groups KE and MP also showed a higher withdrawal threshold to the von Frey fibre compared with group NS (all P < 0.05, Figure 3(b)). The withdrawal threshold to acetone in the ketamine treatment group was significantly higher on the ipsilateral side compared with group NS (P < 0.05) and group MP (all P < 0.05), as shown in Figure 4(a). For the contralateral sides, groups KE and MP also exhibited a significantly higher withdrawal threshold to acetone stimuli compared to group NS (all P < 0.001, Figure 4(b)).


Preventive Treatment with Ketamine Attenuates the Ischaemia-Reperfusion Response in a Chronic Postischaemia Pain Model.

Liman S, Cheung CW, Wong KL, Tai W, Qiu Q, Ng KF, Choi SW, Irwin M - Oxid Med Cell Longev (2015)

Cold allodynia of KE, MP, and NS groups on the ipsilateral (a) and contralateral side (b) from baseline before ischaemia until 7 days after reperfusion. On the ipsilateral side, the withdrawal threshold to acetone of group KE was significantly higher, compared with groups NS (P < 0.05) and MP (P < 0.05). On the contralateral side, the withdrawal threshold to acetone was significantly higher in KE (P < 0.001) and MP (P < 0.001) groups than that of NS; *P < 0.05; ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487903&req=5

fig4: Cold allodynia of KE, MP, and NS groups on the ipsilateral (a) and contralateral side (b) from baseline before ischaemia until 7 days after reperfusion. On the ipsilateral side, the withdrawal threshold to acetone of group KE was significantly higher, compared with groups NS (P < 0.05) and MP (P < 0.05). On the contralateral side, the withdrawal threshold to acetone was significantly higher in KE (P < 0.001) and MP (P < 0.001) groups than that of NS; *P < 0.05; ***P < 0.001.
Mentions: Ketamine significantly alleviated both mechanical and cold allodynia in the ipsilateral hindpaw. On the ipsilateral side, the withdrawal threshold to the von Frey fibre in group KE was significantly higher than that in groups NS and MP (P < 0.01 and P < 0.05, resp., Figure 3(a)). On the contralateral sides, groups KE and MP also showed a higher withdrawal threshold to the von Frey fibre compared with group NS (all P < 0.05, Figure 3(b)). The withdrawal threshold to acetone in the ketamine treatment group was significantly higher on the ipsilateral side compared with group NS (P < 0.05) and group MP (all P < 0.05), as shown in Figure 4(a). For the contralateral sides, groups KE and MP also exhibited a significantly higher withdrawal threshold to acetone stimuli compared to group NS (all P < 0.001, Figure 4(b)).

Bottom Line: Ketamine treated rats showed a significantly lower temperature in the ischaemic hindpaw compared to saline (P < 0.01) and methylprednisolone (P < 0.05) groups.Mechanical and cold allodynia were significantly lower in the ischaemic side in the ketamine group (P < 0.05).In conclusion, immediate administration of ketamine after an ischaemia-reperfusion injury can alleviate pain and inflammation in the CPIP model and has potential to treat postischaemic pain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anaesthesiology, The University of Hong Kong, Pokfulam, Hong Kong.

ABSTRACT
Ischemia and inflammation may be pathophysiological mechanisms of complex regional pain syndrome (CRPS). Ketamine has proposed anti-inflammatory effects and has been used for treating CRPS. This study aimed to evaluate anti-inflammatory and analgesic effects of ketamine after ischaemia-reperfusion injury in a chronic postischaemia pain (CPIP) model of CRPS-I. Using this model, ischemia was induced in the hindlimbs of male Sprague-Dawley rats. Ketamine, methylprednisolone, or saline was administered immediately after reperfusion. Physical effects, (oedema, temperature, and mechanical and cold allodynia) in the bilateral hindpaws, were assessed from 48 hours after reperfusion. Fewer (56%) rats in the ketamine group developed CPIP at the 48th hour after reperfusion (nonsignificant). Ketamine treated rats showed a significantly lower temperature in the ischaemic hindpaw compared to saline (P < 0.01) and methylprednisolone (P < 0.05) groups. Mechanical and cold allodynia were significantly lower in the ischaemic side in the ketamine group (P < 0.05). Proinflammatory cytokines TNF-α and IL-2 were significantly lower at the 48th hour after reperfusion in ketamine and methylprednisolone groups, compared to saline (all P < 0.05). In conclusion, immediate administration of ketamine after an ischaemia-reperfusion injury can alleviate pain and inflammation in the CPIP model and has potential to treat postischaemic pain.

No MeSH data available.


Related in: MedlinePlus