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[(18)F]-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography of LAPC4-CR Castration-Resistant Prostate Cancer Xenograft Model in Soft Tissue Compartments.

McCall KC, Cheng SC, Huang Y, Kohl NE, Tupper T, Van den Abbeele AD, Zukotynski KA, Sweeney CJ - Transl Oncol (2015)

Bottom Line: Preclinical xenograft models have contributed to advancing our understanding of the molecular basis of prostate cancer and to the development of targeted therapy.This study showed that [(18)F]-FDG-PET/CT could be used to image and assess glucose metabolism of LAPC4-CR xenografts in vivo.Further work can investigate the use of PET/CT to quantify the metabolic response of LAPC4-CR to novel agents and combination therapies using soft tissue and possibly bone compartment xenograft models.

View Article: PubMed Central - PubMed

Affiliation: Department of Imaging, Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA, 02215, USA; Center for Biomedical Imaging in Oncology (Lurie Family Imaging Center), Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA, 02215, USA. Electronic address: kmccall@partners.org.

No MeSH data available.


Related in: MedlinePlus

Hematoxylin-stained tissue sections of LAPC4-CR xenografts with the nuclei of tumor cells stained deep purple. Amorphous areas with a lack of staining indicate the acellular regions of necrosis. Slide (a) shows small regions of necrosis within the tumor tissue, whereas (b) shows a tumor that had developed a necrotic core.
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f0030: Hematoxylin-stained tissue sections of LAPC4-CR xenografts with the nuclei of tumor cells stained deep purple. Amorphous areas with a lack of staining indicate the acellular regions of necrosis. Slide (a) shows small regions of necrosis within the tumor tissue, whereas (b) shows a tumor that had developed a necrotic core.

Mentions: A shared reference location defined the coordinates in image space for the PET and CT; this allowed automatic and precise image registration and PET/CT image fusion for image analysis. However, in each mouse, the accuracy of image registration was verified by visual inspection using several images in orthogonal views to ensure that no motion during imaging or errors during reconstruction or fusion had occurred. The fused PET/CT and CT images provided a more accurate estimate of 3D tumor volumes than can be calculated from physical palpation and caliper measurements. In addition, the fused PET/CT images allowed better evaluation of regions of active tumor metabolism. Fused PET/CT images showed regions within the tumor mass where there was little or no [18F]-FDG uptake, often located near the center of the subcutaneous mass, which was a characteristic of soft tissue/tumor necrosis. Other metabolically inactive regions coincided with small localized regions of calcified tissue on the CT images. The presence of necrotic cores and calcification of dead tissue in the LAPC4-CR cell line xenograft tumors was found in earlier histopathology studies, which were done on another cohort of mice during the development of the cell line. Examples of stained tumor sections from that prior study, which illustrates the typical pathology of LAPC4-CR xenografts, are shown in FigureĀ 6.


[(18)F]-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography of LAPC4-CR Castration-Resistant Prostate Cancer Xenograft Model in Soft Tissue Compartments.

McCall KC, Cheng SC, Huang Y, Kohl NE, Tupper T, Van den Abbeele AD, Zukotynski KA, Sweeney CJ - Transl Oncol (2015)

Hematoxylin-stained tissue sections of LAPC4-CR xenografts with the nuclei of tumor cells stained deep purple. Amorphous areas with a lack of staining indicate the acellular regions of necrosis. Slide (a) shows small regions of necrosis within the tumor tissue, whereas (b) shows a tumor that had developed a necrotic core.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487789&req=5

f0030: Hematoxylin-stained tissue sections of LAPC4-CR xenografts with the nuclei of tumor cells stained deep purple. Amorphous areas with a lack of staining indicate the acellular regions of necrosis. Slide (a) shows small regions of necrosis within the tumor tissue, whereas (b) shows a tumor that had developed a necrotic core.
Mentions: A shared reference location defined the coordinates in image space for the PET and CT; this allowed automatic and precise image registration and PET/CT image fusion for image analysis. However, in each mouse, the accuracy of image registration was verified by visual inspection using several images in orthogonal views to ensure that no motion during imaging or errors during reconstruction or fusion had occurred. The fused PET/CT and CT images provided a more accurate estimate of 3D tumor volumes than can be calculated from physical palpation and caliper measurements. In addition, the fused PET/CT images allowed better evaluation of regions of active tumor metabolism. Fused PET/CT images showed regions within the tumor mass where there was little or no [18F]-FDG uptake, often located near the center of the subcutaneous mass, which was a characteristic of soft tissue/tumor necrosis. Other metabolically inactive regions coincided with small localized regions of calcified tissue on the CT images. The presence of necrotic cores and calcification of dead tissue in the LAPC4-CR cell line xenograft tumors was found in earlier histopathology studies, which were done on another cohort of mice during the development of the cell line. Examples of stained tumor sections from that prior study, which illustrates the typical pathology of LAPC4-CR xenografts, are shown in FigureĀ 6.

Bottom Line: Preclinical xenograft models have contributed to advancing our understanding of the molecular basis of prostate cancer and to the development of targeted therapy.This study showed that [(18)F]-FDG-PET/CT could be used to image and assess glucose metabolism of LAPC4-CR xenografts in vivo.Further work can investigate the use of PET/CT to quantify the metabolic response of LAPC4-CR to novel agents and combination therapies using soft tissue and possibly bone compartment xenograft models.

View Article: PubMed Central - PubMed

Affiliation: Department of Imaging, Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA, 02215, USA; Center for Biomedical Imaging in Oncology (Lurie Family Imaging Center), Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA, 02215, USA. Electronic address: kmccall@partners.org.

No MeSH data available.


Related in: MedlinePlus