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Clinical Usefulness of Immunohistochemical Staining of p57 kip2 for the Differential Diagnosis of Complete Mole.

Sasaki S, Sasaki Y, Kunimura T, Sekizawa A, Kojima Y, Iino K - Biomed Res Int (2015)

Bottom Line: Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining.No subsequent changes were observed during follow-up in the other cases.We might be able to avoid the cost of DNA analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Iino Hospital, 4-3-2 Fuda, Chōfu, Tokyo 182-0024, Japan.

ABSTRACT

Objective: Can polymer-based immunohistochemical staining of p57(kip2) replace DNA analysis as an inexpensive means of differentiating complete mole from partial mole or hydropic abortion?

Methods and materials: Original paraffin-embedded tissue blocks from 14 equivocal cases were turned over to our laboratory and examined by immunohistochemical staining of p57(kip2).

Results: Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining. The remaining 10 cases showed apparently positive staining in cytotrophoblasts and villous stromal cells. Without DNA analysis we are able to clearly differentiate the 4 cases of complete mole among the 14 equivocal cases. During follow-up, secondary low-risk gestational trophoblastic neoplasia (GTN) developed in 1 of the 4 cases of complete mole: the GTN was treated by single-agent chemotherapy. No subsequent changes were observed during follow-up in the other cases.

Conclusion: Polymer-based immunohistochemical staining of p57(kip2) (paternally imprinted gene, expressed from maternal allele) is a very effective method that can be used to differentiate androgenetic complete mole from partial mole and hydropic abortion. We might be able to avoid the cost of DNA analysis.

No MeSH data available.


Related in: MedlinePlus

Those figures are the control staining in hydropic abortion (left), partial mole (middle), and complete mole (right). Upper panels show hematoxylin-eosin staining, and middle and lower panels show immunohistochemical staining for p57kip2 by the polymer-based method of control hydropic abortion (left), partial mole (middle), and complete mole (right). Magnification are 100x and 400x by microscope, respectively. In cytotrophoblasts and villous stromal cells, strong positive immunoreactive staining for p57kip2 is seen in the hydropic abortion (left) and partial mole (middle): staining is absent in the complete mole (right). The genetic origins of the control partial and complete mole were established by DNA polymorphism analysis in our laboratory.
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fig1: Those figures are the control staining in hydropic abortion (left), partial mole (middle), and complete mole (right). Upper panels show hematoxylin-eosin staining, and middle and lower panels show immunohistochemical staining for p57kip2 by the polymer-based method of control hydropic abortion (left), partial mole (middle), and complete mole (right). Magnification are 100x and 400x by microscope, respectively. In cytotrophoblasts and villous stromal cells, strong positive immunoreactive staining for p57kip2 is seen in the hydropic abortion (left) and partial mole (middle): staining is absent in the complete mole (right). The genetic origins of the control partial and complete mole were established by DNA polymorphism analysis in our laboratory.

Mentions: Reactivity was judged positive only when distinct nuclear staining of cytotrophoblasts and villous stromal cells was identified. There was no faint nuclear staining observed by polymer-based method through this experiment. Control study showed clearly negative staining of complete moles and positive in partial moles and abortions in cytotrophoblasts and villous stromal cells, respectively. Decidual stromal cells were positive for p57kip2 in all cases and provided a reliable internal control (Figure 1). Syncytiotrophoblasts in complete moles, partial moles, and abortions always stained negatively (Figure 1).


Clinical Usefulness of Immunohistochemical Staining of p57 kip2 for the Differential Diagnosis of Complete Mole.

Sasaki S, Sasaki Y, Kunimura T, Sekizawa A, Kojima Y, Iino K - Biomed Res Int (2015)

Those figures are the control staining in hydropic abortion (left), partial mole (middle), and complete mole (right). Upper panels show hematoxylin-eosin staining, and middle and lower panels show immunohistochemical staining for p57kip2 by the polymer-based method of control hydropic abortion (left), partial mole (middle), and complete mole (right). Magnification are 100x and 400x by microscope, respectively. In cytotrophoblasts and villous stromal cells, strong positive immunoreactive staining for p57kip2 is seen in the hydropic abortion (left) and partial mole (middle): staining is absent in the complete mole (right). The genetic origins of the control partial and complete mole were established by DNA polymorphism analysis in our laboratory.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487724&req=5

fig1: Those figures are the control staining in hydropic abortion (left), partial mole (middle), and complete mole (right). Upper panels show hematoxylin-eosin staining, and middle and lower panels show immunohistochemical staining for p57kip2 by the polymer-based method of control hydropic abortion (left), partial mole (middle), and complete mole (right). Magnification are 100x and 400x by microscope, respectively. In cytotrophoblasts and villous stromal cells, strong positive immunoreactive staining for p57kip2 is seen in the hydropic abortion (left) and partial mole (middle): staining is absent in the complete mole (right). The genetic origins of the control partial and complete mole were established by DNA polymorphism analysis in our laboratory.
Mentions: Reactivity was judged positive only when distinct nuclear staining of cytotrophoblasts and villous stromal cells was identified. There was no faint nuclear staining observed by polymer-based method through this experiment. Control study showed clearly negative staining of complete moles and positive in partial moles and abortions in cytotrophoblasts and villous stromal cells, respectively. Decidual stromal cells were positive for p57kip2 in all cases and provided a reliable internal control (Figure 1). Syncytiotrophoblasts in complete moles, partial moles, and abortions always stained negatively (Figure 1).

Bottom Line: Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining.No subsequent changes were observed during follow-up in the other cases.We might be able to avoid the cost of DNA analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Iino Hospital, 4-3-2 Fuda, Chōfu, Tokyo 182-0024, Japan.

ABSTRACT

Objective: Can polymer-based immunohistochemical staining of p57(kip2) replace DNA analysis as an inexpensive means of differentiating complete mole from partial mole or hydropic abortion?

Methods and materials: Original paraffin-embedded tissue blocks from 14 equivocal cases were turned over to our laboratory and examined by immunohistochemical staining of p57(kip2).

Results: Four of the 14 cases showed clearly negative nuclear staining in cytotrophoblasts and villous stromal cells: these results were fully concordant with the control staining. The remaining 10 cases showed apparently positive staining in cytotrophoblasts and villous stromal cells. Without DNA analysis we are able to clearly differentiate the 4 cases of complete mole among the 14 equivocal cases. During follow-up, secondary low-risk gestational trophoblastic neoplasia (GTN) developed in 1 of the 4 cases of complete mole: the GTN was treated by single-agent chemotherapy. No subsequent changes were observed during follow-up in the other cases.

Conclusion: Polymer-based immunohistochemical staining of p57(kip2) (paternally imprinted gene, expressed from maternal allele) is a very effective method that can be used to differentiate androgenetic complete mole from partial mole and hydropic abortion. We might be able to avoid the cost of DNA analysis.

No MeSH data available.


Related in: MedlinePlus