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Prophylactic Ozone Administration Reduces Intestinal Mucosa Injury Induced by Intestinal Ischemia-Reperfusion in the Rat.

Onal O, Yetisir F, Sarer AE, Zeybek ND, Onal CO, Yurekli B, Celik HT, Sirma A, Kılıc M - Mediators Inflamm. (2015)

Bottom Line: Various beneficial effects of ozone have been shown.TOS parameter was highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group.It is thought that the therapeutic effect of ozone is associated with increase in antioxidant enzymes and protection of cells from oxidation and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Reanimation, Selçuk University Medical Faculty, 42100 Konya, Turkey.

ABSTRACT

Objectives: Intestinal ischemia-reperfusion injury is associated with mucosal damage and has a high rate of mortality. Various beneficial effects of ozone have been shown. The aim of the present study was to show the effects of ozone in ischemia reperfusion model in intestine.

Material and method: Twenty eight Wistar rats were randomized into four groups with seven rats in each group. Control group was administered serum physiologic (SF) intraperitoneally (ip) for five days. Ozone group was administered 1 mg/kg ozone ip for five days. Ischemia Reperfusion (IR) group underwent superior mesenteric artery occlusion for one hour and then reperfusion for two hours. Ozone + IR group was administered 1 mg/kg ozone ip for five days and at sixth day IR model was applied. Rats were anesthetized with ketamine∖xyzlazine and their intracardiac blood was drawn completely and they were sacrificed. Intestinal tissue samples were examined under light microscope. Levels of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), malondyaldehide (MDA), and protein carbonyl (PCO) were analyzed in tissue samples. Total oxidant status (TOS), and total antioxidant capacity (TAC) were analyzed in blood samples. Data were evaluated statistically by Kruskal Wallis test.

Results: In the ozone administered group, degree of intestinal injury was not different from the control group. IR caused an increase in intestinal injury score. The intestinal epithelium maintained its integrity and decrease in intestinal injury score was detected in Ozone + IR group. SOD, GSH-Px, and CAT values were high in ozone group and low in IR. TOS parameter was highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group.

Conclusion: In the present study, IR model caused an increase in intestinal injury.In the present study, ozone administration had an effect improving IR associated tissue injury. In the present study, ozone therapy prevented intestine from ischemia reperfusion injury. It is thought that the therapeutic effect of ozone is associated with increase in antioxidant enzymes and protection of cells from oxidation and inflammation.

No MeSH data available.


Related in: MedlinePlus

Biochemical measurements regarding the study groups. The white and black bars represent control and Ozone groups, respectively. The hatched bars represent the situation after IR injury (diagonal lines). The hatched bars represent the situation after Ozone exposure (horizontal lines) prior to intestinal IR injury.
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fig3: Biochemical measurements regarding the study groups. The white and black bars represent control and Ozone groups, respectively. The hatched bars represent the situation after IR injury (diagonal lines). The hatched bars represent the situation after Ozone exposure (horizontal lines) prior to intestinal IR injury.

Mentions: In addition, SOD, CAT, MDA, GSH-Px, and PCO were evaluated in intestinal tissues; TOS and TAC were measured in blood samples. Although the difference between them was not significant, among the antioxidant parameters, the SOD, GSH-Px, and CAT values were found to be highest in the ozone group and lowest in the IR group. No difference was found between the groups in terms of MDA and PCO. Statistically significant difference was found between terms of TOS and TAC values (p < 0.001). In both TOS and TAC values, significant difference was found between ozone and IR group, control and ozone group, control and IR group, control and IR + ozone group, ozone and IR + ozone group and, and IR and IR + ozone group. The TOS parameter was found to be highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group (Figure 3).


Prophylactic Ozone Administration Reduces Intestinal Mucosa Injury Induced by Intestinal Ischemia-Reperfusion in the Rat.

Onal O, Yetisir F, Sarer AE, Zeybek ND, Onal CO, Yurekli B, Celik HT, Sirma A, Kılıc M - Mediators Inflamm. (2015)

Biochemical measurements regarding the study groups. The white and black bars represent control and Ozone groups, respectively. The hatched bars represent the situation after IR injury (diagonal lines). The hatched bars represent the situation after Ozone exposure (horizontal lines) prior to intestinal IR injury.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487723&req=5

fig3: Biochemical measurements regarding the study groups. The white and black bars represent control and Ozone groups, respectively. The hatched bars represent the situation after IR injury (diagonal lines). The hatched bars represent the situation after Ozone exposure (horizontal lines) prior to intestinal IR injury.
Mentions: In addition, SOD, CAT, MDA, GSH-Px, and PCO were evaluated in intestinal tissues; TOS and TAC were measured in blood samples. Although the difference between them was not significant, among the antioxidant parameters, the SOD, GSH-Px, and CAT values were found to be highest in the ozone group and lowest in the IR group. No difference was found between the groups in terms of MDA and PCO. Statistically significant difference was found between terms of TOS and TAC values (p < 0.001). In both TOS and TAC values, significant difference was found between ozone and IR group, control and ozone group, control and IR group, control and IR + ozone group, ozone and IR + ozone group and, and IR and IR + ozone group. The TOS parameter was found to be highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group (Figure 3).

Bottom Line: Various beneficial effects of ozone have been shown.TOS parameter was highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group.It is thought that the therapeutic effect of ozone is associated with increase in antioxidant enzymes and protection of cells from oxidation and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Reanimation, Selçuk University Medical Faculty, 42100 Konya, Turkey.

ABSTRACT

Objectives: Intestinal ischemia-reperfusion injury is associated with mucosal damage and has a high rate of mortality. Various beneficial effects of ozone have been shown. The aim of the present study was to show the effects of ozone in ischemia reperfusion model in intestine.

Material and method: Twenty eight Wistar rats were randomized into four groups with seven rats in each group. Control group was administered serum physiologic (SF) intraperitoneally (ip) for five days. Ozone group was administered 1 mg/kg ozone ip for five days. Ischemia Reperfusion (IR) group underwent superior mesenteric artery occlusion for one hour and then reperfusion for two hours. Ozone + IR group was administered 1 mg/kg ozone ip for five days and at sixth day IR model was applied. Rats were anesthetized with ketamine∖xyzlazine and their intracardiac blood was drawn completely and they were sacrificed. Intestinal tissue samples were examined under light microscope. Levels of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), malondyaldehide (MDA), and protein carbonyl (PCO) were analyzed in tissue samples. Total oxidant status (TOS), and total antioxidant capacity (TAC) were analyzed in blood samples. Data were evaluated statistically by Kruskal Wallis test.

Results: In the ozone administered group, degree of intestinal injury was not different from the control group. IR caused an increase in intestinal injury score. The intestinal epithelium maintained its integrity and decrease in intestinal injury score was detected in Ozone + IR group. SOD, GSH-Px, and CAT values were high in ozone group and low in IR. TOS parameter was highest in the IR group and the TAC parameter was highest in the ozone group and lowest in the IR group.

Conclusion: In the present study, IR model caused an increase in intestinal injury.In the present study, ozone administration had an effect improving IR associated tissue injury. In the present study, ozone therapy prevented intestine from ischemia reperfusion injury. It is thought that the therapeutic effect of ozone is associated with increase in antioxidant enzymes and protection of cells from oxidation and inflammation.

No MeSH data available.


Related in: MedlinePlus