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Regulation of Neurotrophin-3 and Interleukin-1β and Inhibition of Spinal Glial Activation Contribute to the Analgesic Effect of Electroacupuncture in Chronic Neuropathic Pain States of Rats.

Tu W, Wang W, Xi H, He R, Gao L, Jiang S - Evid Based Complement Alternat Med (2015)

Bottom Line: Electroacupuncture is widely accepted to treat chronic pain, but the precise mechanism underlying the analgesic effect of EA has not been fully demonstrated.Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment.These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.

ABSTRACT
Growing evidence indicates that neurotrophin-3, interleukin-1β, and spinal glia are involved in neuropathic pain derived from dorsal root ganglia to spinal cord. Electroacupuncture is widely accepted to treat chronic pain, but the precise mechanism underlying the analgesic effect of EA has not been fully demonstrated. In this study, the mechanical withdrawal threshold and thermal withdrawal latency were recorded. We used immunofluorescence and western blots methods to investigate the effect of EA on the expression of NT-3 and IL-1β in DRG and spinal cord of CCI rats; we also examined the expression of spinal GFAP and OX-42 in spinal cord. In present study, the MWT and TWL of CCI group rats were lower than those in the Sham CCI group rats, but EA treatment increased the pain thresholds. Furtherly, we found that EA upregulates the expression of NT-3 in DRG and spinal cord of CCI rats, while EA downregulates the expression of IL-1β. Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment. These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

No MeSH data available.


Related in: MedlinePlus

Effect of EA on the increase of IL-1β protein induced by CCI in spinal cord. (a) Results of western blot from spinal cord in each group. (b) Changes in the relative content of IL-1β protein from picture (a). ###P < 0.01, compared with the Sham group; &&&P < 0.001, compared with the CCI group.
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fig5: Effect of EA on the increase of IL-1β protein induced by CCI in spinal cord. (a) Results of western blot from spinal cord in each group. (b) Changes in the relative content of IL-1β protein from picture (a). ###P < 0.01, compared with the Sham group; &&&P < 0.001, compared with the CCI group.

Mentions: The expression of IL-1β in spinal cord was observed through immunofluorescence and western blotting. CCI injury increased the expression of IL-1β in spinal cord (F(2,15) = 50.2, P < 0.001; F(2,15) = 144.6, P < 0.001), compared with the Sham CCI group (Figures 4 and 5). However, in the CCI + EA group, the immunoreactivity and quantitativeness of protein of IL-1β were lower than those in the CCI group (P < 0.001, P < 0.001), implying EA could inhibit the expression of IL-1β in spinal cord of CCI rats.


Regulation of Neurotrophin-3 and Interleukin-1β and Inhibition of Spinal Glial Activation Contribute to the Analgesic Effect of Electroacupuncture in Chronic Neuropathic Pain States of Rats.

Tu W, Wang W, Xi H, He R, Gao L, Jiang S - Evid Based Complement Alternat Med (2015)

Effect of EA on the increase of IL-1β protein induced by CCI in spinal cord. (a) Results of western blot from spinal cord in each group. (b) Changes in the relative content of IL-1β protein from picture (a). ###P < 0.01, compared with the Sham group; &&&P < 0.001, compared with the CCI group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487695&req=5

fig5: Effect of EA on the increase of IL-1β protein induced by CCI in spinal cord. (a) Results of western blot from spinal cord in each group. (b) Changes in the relative content of IL-1β protein from picture (a). ###P < 0.01, compared with the Sham group; &&&P < 0.001, compared with the CCI group.
Mentions: The expression of IL-1β in spinal cord was observed through immunofluorescence and western blotting. CCI injury increased the expression of IL-1β in spinal cord (F(2,15) = 50.2, P < 0.001; F(2,15) = 144.6, P < 0.001), compared with the Sham CCI group (Figures 4 and 5). However, in the CCI + EA group, the immunoreactivity and quantitativeness of protein of IL-1β were lower than those in the CCI group (P < 0.001, P < 0.001), implying EA could inhibit the expression of IL-1β in spinal cord of CCI rats.

Bottom Line: Electroacupuncture is widely accepted to treat chronic pain, but the precise mechanism underlying the analgesic effect of EA has not been fully demonstrated.Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment.These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.

ABSTRACT
Growing evidence indicates that neurotrophin-3, interleukin-1β, and spinal glia are involved in neuropathic pain derived from dorsal root ganglia to spinal cord. Electroacupuncture is widely accepted to treat chronic pain, but the precise mechanism underlying the analgesic effect of EA has not been fully demonstrated. In this study, the mechanical withdrawal threshold and thermal withdrawal latency were recorded. We used immunofluorescence and western blots methods to investigate the effect of EA on the expression of NT-3 and IL-1β in DRG and spinal cord of CCI rats; we also examined the expression of spinal GFAP and OX-42 in spinal cord. In present study, the MWT and TWL of CCI group rats were lower than those in the Sham CCI group rats, but EA treatment increased the pain thresholds. Furtherly, we found that EA upregulates the expression of NT-3 in DRG and spinal cord of CCI rats, while EA downregulates the expression of IL-1β. Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment. These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

No MeSH data available.


Related in: MedlinePlus