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Regulation of Neurotrophin-3 and Interleukin-1β and Inhibition of Spinal Glial Activation Contribute to the Analgesic Effect of Electroacupuncture in Chronic Neuropathic Pain States of Rats.

Tu W, Wang W, Xi H, He R, Gao L, Jiang S - Evid Based Complement Alternat Med (2015)

Bottom Line: In this study, the mechanical withdrawal threshold and thermal withdrawal latency were recorded.Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment.These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.

ABSTRACT
Growing evidence indicates that neurotrophin-3, interleukin-1β, and spinal glia are involved in neuropathic pain derived from dorsal root ganglia to spinal cord. Electroacupuncture is widely accepted to treat chronic pain, but the precise mechanism underlying the analgesic effect of EA has not been fully demonstrated. In this study, the mechanical withdrawal threshold and thermal withdrawal latency were recorded. We used immunofluorescence and western blots methods to investigate the effect of EA on the expression of NT-3 and IL-1β in DRG and spinal cord of CCI rats; we also examined the expression of spinal GFAP and OX-42 in spinal cord. In present study, the MWT and TWL of CCI group rats were lower than those in the Sham CCI group rats, but EA treatment increased the pain thresholds. Furtherly, we found that EA upregulates the expression of NT-3 in DRG and spinal cord of CCI rats, while EA downregulates the expression of IL-1β. Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment. These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

No MeSH data available.


Related in: MedlinePlus

Effect of EA on CCI-induced increase of the quantitative changes of NT-3 protein in spinal cord. (a) Changes in the relative content of NT-3 protein in spinal cord of every group. (b) Quantification of bands showing that EA treatment promoted the expression of NT-3. ###P < 0.001, versus the Sham group; &&&P < 0.001, versus the CCI group.
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fig3: Effect of EA on CCI-induced increase of the quantitative changes of NT-3 protein in spinal cord. (a) Changes in the relative content of NT-3 protein in spinal cord of every group. (b) Quantification of bands showing that EA treatment promoted the expression of NT-3. ###P < 0.001, versus the Sham group; &&&P < 0.001, versus the CCI group.

Mentions: The NT-3 expression at the protein level in spinal cord was analyzed using western blotting. The relative optical density (ROD) value for the NT-3 protein expression in the CCI group was significantly higher than that in Sham CCI group (F(2,15) = 96.2, P < 0.001), and EA treatment increased that ROD in the EA group. Similar to the result of immunohistochemistry, the level of NT-3 protein significantly increased after EA treatment (P < 0.001) (Figures 3(a) and 3(b)).


Regulation of Neurotrophin-3 and Interleukin-1β and Inhibition of Spinal Glial Activation Contribute to the Analgesic Effect of Electroacupuncture in Chronic Neuropathic Pain States of Rats.

Tu W, Wang W, Xi H, He R, Gao L, Jiang S - Evid Based Complement Alternat Med (2015)

Effect of EA on CCI-induced increase of the quantitative changes of NT-3 protein in spinal cord. (a) Changes in the relative content of NT-3 protein in spinal cord of every group. (b) Quantification of bands showing that EA treatment promoted the expression of NT-3. ###P < 0.001, versus the Sham group; &&&P < 0.001, versus the CCI group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487695&req=5

fig3: Effect of EA on CCI-induced increase of the quantitative changes of NT-3 protein in spinal cord. (a) Changes in the relative content of NT-3 protein in spinal cord of every group. (b) Quantification of bands showing that EA treatment promoted the expression of NT-3. ###P < 0.001, versus the Sham group; &&&P < 0.001, versus the CCI group.
Mentions: The NT-3 expression at the protein level in spinal cord was analyzed using western blotting. The relative optical density (ROD) value for the NT-3 protein expression in the CCI group was significantly higher than that in Sham CCI group (F(2,15) = 96.2, P < 0.001), and EA treatment increased that ROD in the EA group. Similar to the result of immunohistochemistry, the level of NT-3 protein significantly increased after EA treatment (P < 0.001) (Figures 3(a) and 3(b)).

Bottom Line: In this study, the mechanical withdrawal threshold and thermal withdrawal latency were recorded.Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment.These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Medicine and Rehabilitation, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.

ABSTRACT
Growing evidence indicates that neurotrophin-3, interleukin-1β, and spinal glia are involved in neuropathic pain derived from dorsal root ganglia to spinal cord. Electroacupuncture is widely accepted to treat chronic pain, but the precise mechanism underlying the analgesic effect of EA has not been fully demonstrated. In this study, the mechanical withdrawal threshold and thermal withdrawal latency were recorded. We used immunofluorescence and western blots methods to investigate the effect of EA on the expression of NT-3 and IL-1β in DRG and spinal cord of CCI rats; we also examined the expression of spinal GFAP and OX-42 in spinal cord. In present study, the MWT and TWL of CCI group rats were lower than those in the Sham CCI group rats, but EA treatment increased the pain thresholds. Furtherly, we found that EA upregulates the expression of NT-3 in DRG and spinal cord of CCI rats, while EA downregulates the expression of IL-1β. Additionally, immunofluorescence exhibited that CCI-induced activation of microglia and astrocytes was inhibited significantly by EA treatment. These results demonstrated that the analgesic effect of EA may be achieved through promoting the neural protection of NT-3 as well as the inhibition of IL-1β production and spinal glial activity.

No MeSH data available.


Related in: MedlinePlus