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Kinetics of Circulating Damage-Associated Molecular Patterns in Sepsis.

Miki T, Iba T - J Immunol Res (2015)

Bottom Line: In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time.The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7).The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency and Disaster Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.

ABSTRACT
Circulating levels of conventional biomarkers and damage-associated molecular patterns were examined in 30 severe sepsis patients (20 survivors and 10 nonsurvivors). Plasma levels of interleukin 6, CRP, and procalcitonin reached their peaks on Day 0 (onset of sepsis) or Day 1 and declined rapidly thereafter despite the persistent severity. In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time. The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7). The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74. Measurement of circulating levels of the histone H3 provides additional information to that of the conventional indicators of inflammation for determining the severity of sepsis.

No MeSH data available.


Related in: MedlinePlus

ROC curve for histone H3 for death and correlation between SAPS II and histone H3. ROC curve analysis calculated AUC for histone H3 on Day 7 for death. AUC was 0.740 (95% CI: 0.513–0.967). The cutoff value of the histone H3 was 0.08 AU (a). No significant correlation between SAPS II score and histone H3 level on Day 7 was recognized (r = −0.01, p = 0.614 (b)). SAPS II: Simplified Acute Physiology Score II, ROC: receiver operating characteristic, AUC: area under the curve, OR: odds ratio, CI: confidence intervals, and AU: absorbance units.
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fig4: ROC curve for histone H3 for death and correlation between SAPS II and histone H3. ROC curve analysis calculated AUC for histone H3 on Day 7 for death. AUC was 0.740 (95% CI: 0.513–0.967). The cutoff value of the histone H3 was 0.08 AU (a). No significant correlation between SAPS II score and histone H3 level on Day 7 was recognized (r = −0.01, p = 0.614 (b)). SAPS II: Simplified Acute Physiology Score II, ROC: receiver operating characteristic, AUC: area under the curve, OR: odds ratio, CI: confidence intervals, and AU: absorbance units.

Mentions: AUCs for IL-6, CRP, PCT, histone H3, nucleosome, and HMGB1 on Day 7 for survival were 0.693 (95% CI: 0.435–0.952), 0.501 (95% CI: 0.287–0.882), 0.667 (95% CI: 0.375–0.958), 0.740 (95% CI: 0.513–0.967, Figure 4(a)), 0.571 (95% CI: 0.296–0.847), and 0.580 (95% CI: 0.225–0.935), respectively. The cutoff value of the histone H3 calculated by the ROC curve analysis was 0.08 AU. No significant correlation between SAPS II score and histone H3 level was recognized (r = −0.01, p = 0.614).


Kinetics of Circulating Damage-Associated Molecular Patterns in Sepsis.

Miki T, Iba T - J Immunol Res (2015)

ROC curve for histone H3 for death and correlation between SAPS II and histone H3. ROC curve analysis calculated AUC for histone H3 on Day 7 for death. AUC was 0.740 (95% CI: 0.513–0.967). The cutoff value of the histone H3 was 0.08 AU (a). No significant correlation between SAPS II score and histone H3 level on Day 7 was recognized (r = −0.01, p = 0.614 (b)). SAPS II: Simplified Acute Physiology Score II, ROC: receiver operating characteristic, AUC: area under the curve, OR: odds ratio, CI: confidence intervals, and AU: absorbance units.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4487694&req=5

fig4: ROC curve for histone H3 for death and correlation between SAPS II and histone H3. ROC curve analysis calculated AUC for histone H3 on Day 7 for death. AUC was 0.740 (95% CI: 0.513–0.967). The cutoff value of the histone H3 was 0.08 AU (a). No significant correlation between SAPS II score and histone H3 level on Day 7 was recognized (r = −0.01, p = 0.614 (b)). SAPS II: Simplified Acute Physiology Score II, ROC: receiver operating characteristic, AUC: area under the curve, OR: odds ratio, CI: confidence intervals, and AU: absorbance units.
Mentions: AUCs for IL-6, CRP, PCT, histone H3, nucleosome, and HMGB1 on Day 7 for survival were 0.693 (95% CI: 0.435–0.952), 0.501 (95% CI: 0.287–0.882), 0.667 (95% CI: 0.375–0.958), 0.740 (95% CI: 0.513–0.967, Figure 4(a)), 0.571 (95% CI: 0.296–0.847), and 0.580 (95% CI: 0.225–0.935), respectively. The cutoff value of the histone H3 calculated by the ROC curve analysis was 0.08 AU. No significant correlation between SAPS II score and histone H3 level was recognized (r = −0.01, p = 0.614).

Bottom Line: In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time.The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7).The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency and Disaster Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.

ABSTRACT
Circulating levels of conventional biomarkers and damage-associated molecular patterns were examined in 30 severe sepsis patients (20 survivors and 10 nonsurvivors). Plasma levels of interleukin 6, CRP, and procalcitonin reached their peaks on Day 0 (onset of sepsis) or Day 1 and declined rapidly thereafter despite the persistent severity. In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time. The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7). The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74. Measurement of circulating levels of the histone H3 provides additional information to that of the conventional indicators of inflammation for determining the severity of sepsis.

No MeSH data available.


Related in: MedlinePlus