Limits...
Kinetics of Circulating Damage-Associated Molecular Patterns in Sepsis.

Miki T, Iba T - J Immunol Res (2015)

Bottom Line: In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time.The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7).The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency and Disaster Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.

ABSTRACT
Circulating levels of conventional biomarkers and damage-associated molecular patterns were examined in 30 severe sepsis patients (20 survivors and 10 nonsurvivors). Plasma levels of interleukin 6, CRP, and procalcitonin reached their peaks on Day 0 (onset of sepsis) or Day 1 and declined rapidly thereafter despite the persistent severity. In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time. The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7). The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74. Measurement of circulating levels of the histone H3 provides additional information to that of the conventional indicators of inflammation for determining the severity of sepsis.

No MeSH data available.


Related in: MedlinePlus

Comparisons of biomarkers between survivors group and nonsurvivors group. The time-courses of various biomarkers in survivors group and nonsurvivors group are plotted ((a) IL-6, (b) CRP, (c) PCT, (d) histone H3, (e) nucleosome, and (f) HMGB1). No difference was observed between the two groups in IL-6, CRP, PCT, nucleosome, and HMGB1, while histone H3 level was higher in nonsurvivors group on Day 7. Data are expressed as means ± standard error. AU: absorbance units, IL-6: interleukin 6, CRP: c-reacting protein, PCT: procalcitonin, and HMGB1: high-mobility group protein Box 1, ∗p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4487694&req=5

fig3: Comparisons of biomarkers between survivors group and nonsurvivors group. The time-courses of various biomarkers in survivors group and nonsurvivors group are plotted ((a) IL-6, (b) CRP, (c) PCT, (d) histone H3, (e) nucleosome, and (f) HMGB1). No difference was observed between the two groups in IL-6, CRP, PCT, nucleosome, and HMGB1, while histone H3 level was higher in nonsurvivors group on Day 7. Data are expressed as means ± standard error. AU: absorbance units, IL-6: interleukin 6, CRP: c-reacting protein, PCT: procalcitonin, and HMGB1: high-mobility group protein Box 1, ∗p < 0.05.

Mentions: The time-courses of changes in the SAPS II score (Figure 1(b)) and plasma levels of each of the biomarkers were compared between the survivor group and nonsurvivor group (Figure 3). The SAPS II score was significantly higher in the nonsurvivor group than in the survivor group on Day 1 (p < 0.05) and Day 7 (p < 0.01). In regard to the plasma levels of IL-6, CRP, PCT, histone H3, nucleosome, and HMGB1, although no statistically significant differences were recognized between the two groups, the plasma IL-6 value on Day 0 was more than 6 times higher in the nonsurvivor group than that in the survivor group. Also, the plasma histone H3 level increased gradually and tended to be higher in the nonsurvivor group on Day 3 (p = 0.06) and Day 7 (p = 0.04).


Kinetics of Circulating Damage-Associated Molecular Patterns in Sepsis.

Miki T, Iba T - J Immunol Res (2015)

Comparisons of biomarkers between survivors group and nonsurvivors group. The time-courses of various biomarkers in survivors group and nonsurvivors group are plotted ((a) IL-6, (b) CRP, (c) PCT, (d) histone H3, (e) nucleosome, and (f) HMGB1). No difference was observed between the two groups in IL-6, CRP, PCT, nucleosome, and HMGB1, while histone H3 level was higher in nonsurvivors group on Day 7. Data are expressed as means ± standard error. AU: absorbance units, IL-6: interleukin 6, CRP: c-reacting protein, PCT: procalcitonin, and HMGB1: high-mobility group protein Box 1, ∗p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4487694&req=5

fig3: Comparisons of biomarkers between survivors group and nonsurvivors group. The time-courses of various biomarkers in survivors group and nonsurvivors group are plotted ((a) IL-6, (b) CRP, (c) PCT, (d) histone H3, (e) nucleosome, and (f) HMGB1). No difference was observed between the two groups in IL-6, CRP, PCT, nucleosome, and HMGB1, while histone H3 level was higher in nonsurvivors group on Day 7. Data are expressed as means ± standard error. AU: absorbance units, IL-6: interleukin 6, CRP: c-reacting protein, PCT: procalcitonin, and HMGB1: high-mobility group protein Box 1, ∗p < 0.05.
Mentions: The time-courses of changes in the SAPS II score (Figure 1(b)) and plasma levels of each of the biomarkers were compared between the survivor group and nonsurvivor group (Figure 3). The SAPS II score was significantly higher in the nonsurvivor group than in the survivor group on Day 1 (p < 0.05) and Day 7 (p < 0.01). In regard to the plasma levels of IL-6, CRP, PCT, histone H3, nucleosome, and HMGB1, although no statistically significant differences were recognized between the two groups, the plasma IL-6 value on Day 0 was more than 6 times higher in the nonsurvivor group than that in the survivor group. Also, the plasma histone H3 level increased gradually and tended to be higher in the nonsurvivor group on Day 3 (p = 0.06) and Day 7 (p = 0.04).

Bottom Line: In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time.The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7).The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency and Disaster Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, Japan.

ABSTRACT
Circulating levels of conventional biomarkers and damage-associated molecular patterns were examined in 30 severe sepsis patients (20 survivors and 10 nonsurvivors). Plasma levels of interleukin 6, CRP, and procalcitonin reached their peaks on Day 0 (onset of sepsis) or Day 1 and declined rapidly thereafter despite the persistent severity. In contrast, elevated levels of histone H3, nucleosome, and high-mobility group protein Box 1 remained for longer periods of time. The peak level of histone H3 in the nonsurvivors was higher than that of the survivors (p < 0.05 on Day 7). The cutoff value of the histone H3 on Day 7 for death was 0.08 AU and the area under the receiver operating characteristic curve showed discriminative powers of 0.74. Measurement of circulating levels of the histone H3 provides additional information to that of the conventional indicators of inflammation for determining the severity of sepsis.

No MeSH data available.


Related in: MedlinePlus