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Seasonal Dynamics of Marine Microbial Community in the South Sea of Korea.

Suh SS, Park M, Hwang J, Kil EJ, Jung SW, Lee S, Lee TK - PLoS ONE (2015)

Bottom Line: In addition, Psedoalteromonadaceae, Vibrionaceae and SAR11-1 were predominant members of the OTUs found in all sampling seasons.Environmental factors significantly influenced the bacterial community structure among season, with the phosphate and nitrate concentrations contributing strongly to the spatial distribution of the Alphaproteobacteria; the Gammaproteobacteria, Flavobacteria, and Actinobacteria all showed marked negative correlations with all measured nutrients, particularly silicon dioxide and chlorophyll-a.The results suggest that seasonal changes in environmental variables contribute to the dynamic structure of the bacterial community in the study area.

View Article: PubMed Central - PubMed

Affiliation: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje, 656-830, Republic of Korea.

ABSTRACT
High-resolution 16S rRNA tag pyrosequencing was used to obtain seasonal snapshots of the bacterial diversity and community structure at two locations in Gosung Bay (South Sea, Korea) over a one year period. Seasonal sampling from the water column at each site revealed highly diverse bacterial communities containing up to 900 estimated Operational Taxonomic Units (OTUs). The Alphaproteobacteria and Gammaproteobacteria were the most abundant groups, and the most frequently recorded OTUs were members of Pelagibacter and Glaciecola. In particular, it was observed that Arcobacter, a genus of the Epsilonproteobacteria, dominated during summer. In addition, Psedoalteromonadaceae, Vibrionaceae and SAR11-1 were predominant members of the OTUs found in all sampling seasons. Environmental factors significantly influenced the bacterial community structure among season, with the phosphate and nitrate concentrations contributing strongly to the spatial distribution of the Alphaproteobacteria; the Gammaproteobacteria, Flavobacteria, and Actinobacteria all showed marked negative correlations with all measured nutrients, particularly silicon dioxide and chlorophyll-a. The results suggest that seasonal changes in environmental variables contribute to the dynamic structure of the bacterial community in the study area.

No MeSH data available.


Related in: MedlinePlus

Maps showing the sampling locations in the South Sea of Korea.
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pone.0131633.g001: Maps showing the sampling locations in the South Sea of Korea.

Mentions: To determine the composition and distribution of bacteria in the water column, seasonal samples were collected at two sites in Gosung Bay (Fig 1); environmental factors including temperature, dissolved oxygen (DO), chlorophyll-a and various nutrients differed significantly among the seasons (one-way ANOVA; P < 0.05) (Table 1). Pyrosequencing was performed on the mixed PCR amplicons generated from the pooled DNA from each site. A total of 53,592 reads (average number of reads per sample, 6699; average read length, 372 bp) were recovered following quality control filtering, and clustered into 15,138 OTUs (97% of the qualified reads; Table 2). The numbers of OTUs in winter, spring, summer and autumn in Gosung Bay were 1408, 1989, 1536 and 1235 OTUs, respectively. On the basis of the nonparametric Chao1 index, the richness of the entire bacterial community was highest during spring, while the bacterial diversity appeared to be similar between the sampling sites and among seasons, based on the Shannon diversity index; the exception was the spring water column, which showed much more bacterial diversity than in the other seasons.


Seasonal Dynamics of Marine Microbial Community in the South Sea of Korea.

Suh SS, Park M, Hwang J, Kil EJ, Jung SW, Lee S, Lee TK - PLoS ONE (2015)

Maps showing the sampling locations in the South Sea of Korea.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487691&req=5

pone.0131633.g001: Maps showing the sampling locations in the South Sea of Korea.
Mentions: To determine the composition and distribution of bacteria in the water column, seasonal samples were collected at two sites in Gosung Bay (Fig 1); environmental factors including temperature, dissolved oxygen (DO), chlorophyll-a and various nutrients differed significantly among the seasons (one-way ANOVA; P < 0.05) (Table 1). Pyrosequencing was performed on the mixed PCR amplicons generated from the pooled DNA from each site. A total of 53,592 reads (average number of reads per sample, 6699; average read length, 372 bp) were recovered following quality control filtering, and clustered into 15,138 OTUs (97% of the qualified reads; Table 2). The numbers of OTUs in winter, spring, summer and autumn in Gosung Bay were 1408, 1989, 1536 and 1235 OTUs, respectively. On the basis of the nonparametric Chao1 index, the richness of the entire bacterial community was highest during spring, while the bacterial diversity appeared to be similar between the sampling sites and among seasons, based on the Shannon diversity index; the exception was the spring water column, which showed much more bacterial diversity than in the other seasons.

Bottom Line: In addition, Psedoalteromonadaceae, Vibrionaceae and SAR11-1 were predominant members of the OTUs found in all sampling seasons.Environmental factors significantly influenced the bacterial community structure among season, with the phosphate and nitrate concentrations contributing strongly to the spatial distribution of the Alphaproteobacteria; the Gammaproteobacteria, Flavobacteria, and Actinobacteria all showed marked negative correlations with all measured nutrients, particularly silicon dioxide and chlorophyll-a.The results suggest that seasonal changes in environmental variables contribute to the dynamic structure of the bacterial community in the study area.

View Article: PubMed Central - PubMed

Affiliation: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje, 656-830, Republic of Korea.

ABSTRACT
High-resolution 16S rRNA tag pyrosequencing was used to obtain seasonal snapshots of the bacterial diversity and community structure at two locations in Gosung Bay (South Sea, Korea) over a one year period. Seasonal sampling from the water column at each site revealed highly diverse bacterial communities containing up to 900 estimated Operational Taxonomic Units (OTUs). The Alphaproteobacteria and Gammaproteobacteria were the most abundant groups, and the most frequently recorded OTUs were members of Pelagibacter and Glaciecola. In particular, it was observed that Arcobacter, a genus of the Epsilonproteobacteria, dominated during summer. In addition, Psedoalteromonadaceae, Vibrionaceae and SAR11-1 were predominant members of the OTUs found in all sampling seasons. Environmental factors significantly influenced the bacterial community structure among season, with the phosphate and nitrate concentrations contributing strongly to the spatial distribution of the Alphaproteobacteria; the Gammaproteobacteria, Flavobacteria, and Actinobacteria all showed marked negative correlations with all measured nutrients, particularly silicon dioxide and chlorophyll-a. The results suggest that seasonal changes in environmental variables contribute to the dynamic structure of the bacterial community in the study area.

No MeSH data available.


Related in: MedlinePlus