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MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma.

Bai Z, Sun J, Wang X, Wang H, Pei H, Zhang Z - Oncol. Rep. (2015)

Bottom Line: The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues.Notably, SNAI1 was identified as a direct target of miR-153 in PDAC.In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

ABSTRACT
Human pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with early metastasis, which leads to poor prognosis for patients. Mounting evidence suggests that microRNAs (miRNAs) act as critical factors for tumor recurrence and metastasis. miR-153 has been suggested as a novel tumor-associated miRNA, which is involved in tumor metastasis. However, the clinical significance of miR-153 and its role in PDAC remains to be investigated. The aim of the present study was to investigate the expression levels of miR-153 using RT-qPCR in human PDAC cell lines and tissues. A clinical association analysis was performed to investigate the clinical significance of miR-153. The results showed that, the relative expression of miR-153 in PDAC cells was obviously decreased as compared to that in the normal human pancreatic duct epithelial cell line. The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues. The clinical analysis revealed that a low expression of miR-153 was closely associated with poor prognostic features and shorter long-term survival of PDAC patients. Furthermore, univariate and multivariate Cox regression analyses showed that miR-153 was an independent prognostic factor for predicting survival in PDAC patients. In vitro studies demonstrated that the upregulation of miR-153 inhibited migration and invasion in MIAPaCa-2 cells. By contrast, the downregulation of miR-153 increased the number of migrated and invaded AsPC-1 cells. miR-153 inversely regulated SNAI1 abundance in MIAPaCa-2 cells. Notably, SNAI1 was identified as a direct target of miR-153 in PDAC. Furthermore, an inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

No MeSH data available.


Related in: MedlinePlus

An inverse correlation between miR-153 and SNAI1 expression is observed in PDAC. (A) A comparison of differences in the expression levels of SNAI1 protein between PDAC tissues and normal pancreas tissues was performed. *P<0.05. (B) A significant inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. Representative immunostaining showed a weak expression of SNAI1 in miR-153 high-expressing PDAC tissue and a strong expression of SNAI1 in miR-153 low-expressing tumor. *P<0.05. Scale bar, 50 µm. PDAC, pancreatic ductal adenocarcinoma.
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f5-or-34-02-0595: An inverse correlation between miR-153 and SNAI1 expression is observed in PDAC. (A) A comparison of differences in the expression levels of SNAI1 protein between PDAC tissues and normal pancreas tissues was performed. *P<0.05. (B) A significant inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. Representative immunostaining showed a weak expression of SNAI1 in miR-153 high-expressing PDAC tissue and a strong expression of SNAI1 in miR-153 low-expressing tumor. *P<0.05. Scale bar, 50 µm. PDAC, pancreatic ductal adenocarcinoma.

Mentions: Expression of SNAI1 was detected by immunohisto-chemistry in the previous cohort of 80 cases of PDAC tissues and 30 cases of normal pancreas tissues. The mean level of SNAI1 protein was demonstrated to be significantly higher in PDAC tissues as compared with the normal pancreas tissues (P<0.05, Fig. 5A). SNAI1 immunoreactivity was considered as either negative (score 0) or positive (scores 1–3). In these cases, SNAI1 expression was detected in 75.0% (30/40) of the PDAC samples with a low expression of miR-153, whereas only 42.5% (17/40) of the PDAC specimens with a high expression of miR-153 showed a positive SNAI1 signal (P<0.05, Fig. 5B). Furthermore, Spearman’s correlation analysis indicated that miR-153 was inversely correlated with SNAI1 expression in PDAC tissues (r=−0.563, P<0.001).


MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma.

Bai Z, Sun J, Wang X, Wang H, Pei H, Zhang Z - Oncol. Rep. (2015)

An inverse correlation between miR-153 and SNAI1 expression is observed in PDAC. (A) A comparison of differences in the expression levels of SNAI1 protein between PDAC tissues and normal pancreas tissues was performed. *P<0.05. (B) A significant inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. Representative immunostaining showed a weak expression of SNAI1 in miR-153 high-expressing PDAC tissue and a strong expression of SNAI1 in miR-153 low-expressing tumor. *P<0.05. Scale bar, 50 µm. PDAC, pancreatic ductal adenocarcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487667&req=5

f5-or-34-02-0595: An inverse correlation between miR-153 and SNAI1 expression is observed in PDAC. (A) A comparison of differences in the expression levels of SNAI1 protein between PDAC tissues and normal pancreas tissues was performed. *P<0.05. (B) A significant inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. Representative immunostaining showed a weak expression of SNAI1 in miR-153 high-expressing PDAC tissue and a strong expression of SNAI1 in miR-153 low-expressing tumor. *P<0.05. Scale bar, 50 µm. PDAC, pancreatic ductal adenocarcinoma.
Mentions: Expression of SNAI1 was detected by immunohisto-chemistry in the previous cohort of 80 cases of PDAC tissues and 30 cases of normal pancreas tissues. The mean level of SNAI1 protein was demonstrated to be significantly higher in PDAC tissues as compared with the normal pancreas tissues (P<0.05, Fig. 5A). SNAI1 immunoreactivity was considered as either negative (score 0) or positive (scores 1–3). In these cases, SNAI1 expression was detected in 75.0% (30/40) of the PDAC samples with a low expression of miR-153, whereas only 42.5% (17/40) of the PDAC specimens with a high expression of miR-153 showed a positive SNAI1 signal (P<0.05, Fig. 5B). Furthermore, Spearman’s correlation analysis indicated that miR-153 was inversely correlated with SNAI1 expression in PDAC tissues (r=−0.563, P<0.001).

Bottom Line: The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues.Notably, SNAI1 was identified as a direct target of miR-153 in PDAC.In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

ABSTRACT
Human pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with early metastasis, which leads to poor prognosis for patients. Mounting evidence suggests that microRNAs (miRNAs) act as critical factors for tumor recurrence and metastasis. miR-153 has been suggested as a novel tumor-associated miRNA, which is involved in tumor metastasis. However, the clinical significance of miR-153 and its role in PDAC remains to be investigated. The aim of the present study was to investigate the expression levels of miR-153 using RT-qPCR in human PDAC cell lines and tissues. A clinical association analysis was performed to investigate the clinical significance of miR-153. The results showed that, the relative expression of miR-153 in PDAC cells was obviously decreased as compared to that in the normal human pancreatic duct epithelial cell line. The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues. The clinical analysis revealed that a low expression of miR-153 was closely associated with poor prognostic features and shorter long-term survival of PDAC patients. Furthermore, univariate and multivariate Cox regression analyses showed that miR-153 was an independent prognostic factor for predicting survival in PDAC patients. In vitro studies demonstrated that the upregulation of miR-153 inhibited migration and invasion in MIAPaCa-2 cells. By contrast, the downregulation of miR-153 increased the number of migrated and invaded AsPC-1 cells. miR-153 inversely regulated SNAI1 abundance in MIAPaCa-2 cells. Notably, SNAI1 was identified as a direct target of miR-153 in PDAC. Furthermore, an inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

No MeSH data available.


Related in: MedlinePlus