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MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma.

Bai Z, Sun J, Wang X, Wang H, Pei H, Zhang Z - Oncol. Rep. (2015)

Bottom Line: The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues.Notably, SNAI1 was identified as a direct target of miR-153 in PDAC.In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

ABSTRACT
Human pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with early metastasis, which leads to poor prognosis for patients. Mounting evidence suggests that microRNAs (miRNAs) act as critical factors for tumor recurrence and metastasis. miR-153 has been suggested as a novel tumor-associated miRNA, which is involved in tumor metastasis. However, the clinical significance of miR-153 and its role in PDAC remains to be investigated. The aim of the present study was to investigate the expression levels of miR-153 using RT-qPCR in human PDAC cell lines and tissues. A clinical association analysis was performed to investigate the clinical significance of miR-153. The results showed that, the relative expression of miR-153 in PDAC cells was obviously decreased as compared to that in the normal human pancreatic duct epithelial cell line. The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues. The clinical analysis revealed that a low expression of miR-153 was closely associated with poor prognostic features and shorter long-term survival of PDAC patients. Furthermore, univariate and multivariate Cox regression analyses showed that miR-153 was an independent prognostic factor for predicting survival in PDAC patients. In vitro studies demonstrated that the upregulation of miR-153 inhibited migration and invasion in MIAPaCa-2 cells. By contrast, the downregulation of miR-153 increased the number of migrated and invaded AsPC-1 cells. miR-153 inversely regulated SNAI1 abundance in MIAPaCa-2 cells. Notably, SNAI1 was identified as a direct target of miR-153 in PDAC. Furthermore, an inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

No MeSH data available.


Related in: MedlinePlus

The overall survival (OS) and recurrence-free survival (RFS) rates were estimated by the Kaplan-Meier method. According to the level of miR-153 expression, Kaplan-Meier 3-year OS and RFS curves of PDAC patients showed that a low expression of miR-153 was significantly correlated with poor prognosis. The median expression value obtained for miR-153 of the 80 PDAC samples detected by RT-qPCR was selected as the cut-off value. PDAC, pancreatic ductal adenocarcinoma.
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f2-or-34-02-0595: The overall survival (OS) and recurrence-free survival (RFS) rates were estimated by the Kaplan-Meier method. According to the level of miR-153 expression, Kaplan-Meier 3-year OS and RFS curves of PDAC patients showed that a low expression of miR-153 was significantly correlated with poor prognosis. The median expression value obtained for miR-153 of the 80 PDAC samples detected by RT-qPCR was selected as the cut-off value. PDAC, pancreatic ductal adenocarcinoma.

Mentions: The expression of miR-153 was considered as either low (n=40) or high (n=40) according to the cut-off value, which was defined as the median of the cohort. The association between miR-153 expression and clinicopathological variables of PDAC patients are presented in Table I. Results of the statistical analysis revealed that, miR-153 was expressed at prominent lower levels in patients with lymph node metastasis, tumor recurrence, poor tumor differentiation and advanced tumor stage (P<0.05, respectively). The Kaplan-Meier survival analysis was performed to determine the prognostic value of miR-153 in PDAC patients. PDAC patients with a low expression of miR-153 had an evidently lower 3-year overall survival (OS) as compared with those with a high expression of miR-153 (P=0.041, Fig. 2). Furthermore, it was shown that patients with a low expression of miR-153 were associated with shorter recurrence-free survival (RFS) (P=0.039, Fig. 2). In addition, a univariate Cox regression analysis indicated that clinicopathological characteristics, including tumor differentiation and stage, lymph node metastasis, tumor recurrence and miR-153 expression, were significantly associated with OS (P<0.05, respectively, Table II). Notably, the multivariate Cox regression analysis indicated that miR-153 expression was an independent factor for predicting the 3-year OS of PDAC patients (P=0.038, Table II). These data indicated that miR-153 is a potent biomarker for predicting the prognosis of PDAC patients.


MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma.

Bai Z, Sun J, Wang X, Wang H, Pei H, Zhang Z - Oncol. Rep. (2015)

The overall survival (OS) and recurrence-free survival (RFS) rates were estimated by the Kaplan-Meier method. According to the level of miR-153 expression, Kaplan-Meier 3-year OS and RFS curves of PDAC patients showed that a low expression of miR-153 was significantly correlated with poor prognosis. The median expression value obtained for miR-153 of the 80 PDAC samples detected by RT-qPCR was selected as the cut-off value. PDAC, pancreatic ductal adenocarcinoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487667&req=5

f2-or-34-02-0595: The overall survival (OS) and recurrence-free survival (RFS) rates were estimated by the Kaplan-Meier method. According to the level of miR-153 expression, Kaplan-Meier 3-year OS and RFS curves of PDAC patients showed that a low expression of miR-153 was significantly correlated with poor prognosis. The median expression value obtained for miR-153 of the 80 PDAC samples detected by RT-qPCR was selected as the cut-off value. PDAC, pancreatic ductal adenocarcinoma.
Mentions: The expression of miR-153 was considered as either low (n=40) or high (n=40) according to the cut-off value, which was defined as the median of the cohort. The association between miR-153 expression and clinicopathological variables of PDAC patients are presented in Table I. Results of the statistical analysis revealed that, miR-153 was expressed at prominent lower levels in patients with lymph node metastasis, tumor recurrence, poor tumor differentiation and advanced tumor stage (P<0.05, respectively). The Kaplan-Meier survival analysis was performed to determine the prognostic value of miR-153 in PDAC patients. PDAC patients with a low expression of miR-153 had an evidently lower 3-year overall survival (OS) as compared with those with a high expression of miR-153 (P=0.041, Fig. 2). Furthermore, it was shown that patients with a low expression of miR-153 were associated with shorter recurrence-free survival (RFS) (P=0.039, Fig. 2). In addition, a univariate Cox regression analysis indicated that clinicopathological characteristics, including tumor differentiation and stage, lymph node metastasis, tumor recurrence and miR-153 expression, were significantly associated with OS (P<0.05, respectively, Table II). Notably, the multivariate Cox regression analysis indicated that miR-153 expression was an independent factor for predicting the 3-year OS of PDAC patients (P=0.038, Table II). These data indicated that miR-153 is a potent biomarker for predicting the prognosis of PDAC patients.

Bottom Line: The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues.Notably, SNAI1 was identified as a direct target of miR-153 in PDAC.In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

ABSTRACT
Human pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with early metastasis, which leads to poor prognosis for patients. Mounting evidence suggests that microRNAs (miRNAs) act as critical factors for tumor recurrence and metastasis. miR-153 has been suggested as a novel tumor-associated miRNA, which is involved in tumor metastasis. However, the clinical significance of miR-153 and its role in PDAC remains to be investigated. The aim of the present study was to investigate the expression levels of miR-153 using RT-qPCR in human PDAC cell lines and tissues. A clinical association analysis was performed to investigate the clinical significance of miR-153. The results showed that, the relative expression of miR-153 in PDAC cells was obviously decreased as compared to that in the normal human pancreatic duct epithelial cell line. The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues. The clinical analysis revealed that a low expression of miR-153 was closely associated with poor prognostic features and shorter long-term survival of PDAC patients. Furthermore, univariate and multivariate Cox regression analyses showed that miR-153 was an independent prognostic factor for predicting survival in PDAC patients. In vitro studies demonstrated that the upregulation of miR-153 inhibited migration and invasion in MIAPaCa-2 cells. By contrast, the downregulation of miR-153 increased the number of migrated and invaded AsPC-1 cells. miR-153 inversely regulated SNAI1 abundance in MIAPaCa-2 cells. Notably, SNAI1 was identified as a direct target of miR-153 in PDAC. Furthermore, an inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

No MeSH data available.


Related in: MedlinePlus