Limits...
Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model.

Permuy M, Guede D, López-Peña M, Muñoz F, Caeiro JR, González-Cantalapiedra A - BMC Vet. Res. (2015)

Bottom Line: Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).In the osteoarthritis diacerein-treated group the results showed an improvement with respect to the OA placebo group in all parameters, although the results were not statistically significant.The results of this animal study suggested that the diacerein treatment for OA may be able to ameliorate the swelling and surface alterations of the cartilage and exert an anti-inflammatory effect on the synovial membrane, which might contribute to OA improvement, as well as an anabolic effect on subchondral trabecular bone.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Clinical Sciences, University of Santiago de Compostela (USC), Campus Universitario, s/n, 27002, Lugo, Spain. maria.permuy@usc.es.

ABSTRACT

Background: Osteoarthritis is thought to be the most prevalent chronic and disabling joint disease in animals and humans. At present, there is no ideal treatment option. The aim of this study was to assess the effects of the treatment with oral diacerein on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of osteoarthritis by micro-CT evaluation and histological analysis. To this purpose, osteoarthritis was surgically induced on one knee of 16 rabbits using the contralateral knee as healthy controls. Treatment was started three weeks later and lasted eight weeks. Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).

Results: Sample analysis revealed that this model induced osteoarthritis in the operated knee joint. Osteoarthritis placebo group showed a significant increase in non-calcified cartilage thickness and volume with respect to the control placebo group and important changes in the synovial membrane; whereas the parameters measured in subchondral bone remained unchanged. In the osteoarthritis diacerein-treated group the results showed an improvement with respect to the OA placebo group in all parameters, although the results were not statistically significant.

Conclusion: The results of this animal study suggested that the diacerein treatment for OA may be able to ameliorate the swelling and surface alterations of the cartilage and exert an anti-inflammatory effect on the synovial membrane, which might contribute to OA improvement, as well as an anabolic effect on subchondral trabecular bone.

No MeSH data available.


Related in: MedlinePlus

Box plot comparing the scores obtained by the different experimental groups for qualitative microscopic grading of cartilage and synovial alterations (decalcified samples). Outside values were defined as the values that were smaller than the lower quartile minus 1.5 times the interquartile range or larger than the upper quartile plus 1.5 times the interquartile range (inner fences). CTRL: healthy + placebo; OA: osteoarthritis + placebo; CTRL + DC: healthy + diacerein; OA + DC: osteoarthritis + diacerein. All parameters showed a non-normal distribution. Statistical significance p < 0.05: a vs. CTRL; b vs. OA; c vs. CTRL + DC; d vs. OA + DC
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4487570&req=5

Fig4: Box plot comparing the scores obtained by the different experimental groups for qualitative microscopic grading of cartilage and synovial alterations (decalcified samples). Outside values were defined as the values that were smaller than the lower quartile minus 1.5 times the interquartile range or larger than the upper quartile plus 1.5 times the interquartile range (inner fences). CTRL: healthy + placebo; OA: osteoarthritis + placebo; CTRL + DC: healthy + diacerein; OA + DC: osteoarthritis + diacerein. All parameters showed a non-normal distribution. Statistical significance p < 0.05: a vs. CTRL; b vs. OA; c vs. CTRL + DC; d vs. OA + DC

Mentions: The values of microscopic grading for the decalcified cartilage samples and for the synovial membrane are shown in Table 2 and Fig. 4.Table 2


Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model.

Permuy M, Guede D, López-Peña M, Muñoz F, Caeiro JR, González-Cantalapiedra A - BMC Vet. Res. (2015)

Box plot comparing the scores obtained by the different experimental groups for qualitative microscopic grading of cartilage and synovial alterations (decalcified samples). Outside values were defined as the values that were smaller than the lower quartile minus 1.5 times the interquartile range or larger than the upper quartile plus 1.5 times the interquartile range (inner fences). CTRL: healthy + placebo; OA: osteoarthritis + placebo; CTRL + DC: healthy + diacerein; OA + DC: osteoarthritis + diacerein. All parameters showed a non-normal distribution. Statistical significance p < 0.05: a vs. CTRL; b vs. OA; c vs. CTRL + DC; d vs. OA + DC
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4487570&req=5

Fig4: Box plot comparing the scores obtained by the different experimental groups for qualitative microscopic grading of cartilage and synovial alterations (decalcified samples). Outside values were defined as the values that were smaller than the lower quartile minus 1.5 times the interquartile range or larger than the upper quartile plus 1.5 times the interquartile range (inner fences). CTRL: healthy + placebo; OA: osteoarthritis + placebo; CTRL + DC: healthy + diacerein; OA + DC: osteoarthritis + diacerein. All parameters showed a non-normal distribution. Statistical significance p < 0.05: a vs. CTRL; b vs. OA; c vs. CTRL + DC; d vs. OA + DC
Mentions: The values of microscopic grading for the decalcified cartilage samples and for the synovial membrane are shown in Table 2 and Fig. 4.Table 2

Bottom Line: Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).In the osteoarthritis diacerein-treated group the results showed an improvement with respect to the OA placebo group in all parameters, although the results were not statistically significant.The results of this animal study suggested that the diacerein treatment for OA may be able to ameliorate the swelling and surface alterations of the cartilage and exert an anti-inflammatory effect on the synovial membrane, which might contribute to OA improvement, as well as an anabolic effect on subchondral trabecular bone.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Clinical Sciences, University of Santiago de Compostela (USC), Campus Universitario, s/n, 27002, Lugo, Spain. maria.permuy@usc.es.

ABSTRACT

Background: Osteoarthritis is thought to be the most prevalent chronic and disabling joint disease in animals and humans. At present, there is no ideal treatment option. The aim of this study was to assess the effects of the treatment with oral diacerein on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of osteoarthritis by micro-CT evaluation and histological analysis. To this purpose, osteoarthritis was surgically induced on one knee of 16 rabbits using the contralateral knee as healthy controls. Treatment was started three weeks later and lasted eight weeks. Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).

Results: Sample analysis revealed that this model induced osteoarthritis in the operated knee joint. Osteoarthritis placebo group showed a significant increase in non-calcified cartilage thickness and volume with respect to the control placebo group and important changes in the synovial membrane; whereas the parameters measured in subchondral bone remained unchanged. In the osteoarthritis diacerein-treated group the results showed an improvement with respect to the OA placebo group in all parameters, although the results were not statistically significant.

Conclusion: The results of this animal study suggested that the diacerein treatment for OA may be able to ameliorate the swelling and surface alterations of the cartilage and exert an anti-inflammatory effect on the synovial membrane, which might contribute to OA improvement, as well as an anabolic effect on subchondral trabecular bone.

No MeSH data available.


Related in: MedlinePlus