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[18F]FDG Accumulation in Early Coronary Atherosclerotic Lesions in Pigs.

Tarkia M, Saraste A, Stark C, Vähäsilta T, Savunen T, Strandberg M, Saunavaara V, Tolvanen T, Teuho J, Teräs M, Metsälä O, Rinne P, Heinonen I, Savisto N, Pietilä M, Saukko P, Roivainen A, Knuuti J - PLoS ONE (2015)

Bottom Line: We found increased uptake of [18F]FDG in coronary atherosclerotic lesions in a pig model.However, uptake in these early stage lesions was not detectable with in vivo PET imaging.Further studies are needed to clarify whether visible [18F]FDG uptake in coronary arteries represents more advanced, highly inflamed plaques.

View Article: PubMed Central - PubMed

Affiliation: Turku PET Centre, University of Turku and Turku University Hospital, Turku, Finland.

ABSTRACT

Objective: Inflammation is an important contributor to atherosclerosis progression. A glucose analogue 18F-fluorodeoxyglucose ([18F]FDG) has been used to detect atherosclerotic inflammation. However, it is not known to what extent [18F]FDG is taken up in different stages of atherosclerosis. We aimed to study the uptake of [18F]FDG to various stages of coronary plaques in a pig model.

Methods: First, diabetes was caused by streptozotocin injections (50 mg/kg for 3 days) in farm pigs (n = 10). After 6 months on high-fat diet, pigs underwent dual-gated cardiac PET/CT to measure [18F]FDG uptake in coronary arteries. Coronary segments (n = 33) were harvested for ex vivo measurement of radioactivity and autoradiography (ARG).

Results: Intimal thickening was observed in 16 segments and atheroma type plaques in 10 segments. Compared with the normal vessel wall, ARG showed 1.7±0.7 times higher [18F]FDG accumulation in the intimal thickening and 4.1±2.3 times higher in the atheromas (P = 0.004 and P = 0.003, respectively). Ex vivo mean vessel-to-blood ratio was higher in segments with atheroma than those without atherosclerosis (2.6±1.2 vs. 1.3±0.7, P = 0.04). In vivo PET imaging showed the highest target-to-background ratio (TBR) of 2.7. However, maximum TBR was not significantly different in segments without atherosclerosis (1.1±0.5) and either intimal thickening (1.2±0.4, P = 1.0) or atheroma (1.6±0.6, P = 0.4).

Conclusions: We found increased uptake of [18F]FDG in coronary atherosclerotic lesions in a pig model. However, uptake in these early stage lesions was not detectable with in vivo PET imaging. Further studies are needed to clarify whether visible [18F]FDG uptake in coronary arteries represents more advanced, highly inflamed plaques.

No MeSH data available.


Related in: MedlinePlus

Representative example of fused axial coronary CTA and dual-gated [18F]FDG PET images showing cross-section of the proximal right coronary artery (arrow) in an animal with atheroma.[18F]FDG accumulation in the coronary arteries was measured using CTA as an anatomical reference.
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pone.0131332.g004: Representative example of fused axial coronary CTA and dual-gated [18F]FDG PET images showing cross-section of the proximal right coronary artery (arrow) in an animal with atheroma.[18F]FDG accumulation in the coronary arteries was measured using CTA as an anatomical reference.

Mentions: Dual-gated cardiac PET images showed [18F]FDG accumulation above the blood pool radioactivity co-localizing with the coronary arteries as seen in the co-registered CTA image (Fig 4). In the fasting state, [18F]FDG uptake in the myocardium was low and areas of coronary uptake were clearly separate from myocardium. The highest target-to-background ratio, i.e. vessel uptake normalized to blood pool was 2.7 (2.0 in non-gated PET).


[18F]FDG Accumulation in Early Coronary Atherosclerotic Lesions in Pigs.

Tarkia M, Saraste A, Stark C, Vähäsilta T, Savunen T, Strandberg M, Saunavaara V, Tolvanen T, Teuho J, Teräs M, Metsälä O, Rinne P, Heinonen I, Savisto N, Pietilä M, Saukko P, Roivainen A, Knuuti J - PLoS ONE (2015)

Representative example of fused axial coronary CTA and dual-gated [18F]FDG PET images showing cross-section of the proximal right coronary artery (arrow) in an animal with atheroma.[18F]FDG accumulation in the coronary arteries was measured using CTA as an anatomical reference.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487365&req=5

pone.0131332.g004: Representative example of fused axial coronary CTA and dual-gated [18F]FDG PET images showing cross-section of the proximal right coronary artery (arrow) in an animal with atheroma.[18F]FDG accumulation in the coronary arteries was measured using CTA as an anatomical reference.
Mentions: Dual-gated cardiac PET images showed [18F]FDG accumulation above the blood pool radioactivity co-localizing with the coronary arteries as seen in the co-registered CTA image (Fig 4). In the fasting state, [18F]FDG uptake in the myocardium was low and areas of coronary uptake were clearly separate from myocardium. The highest target-to-background ratio, i.e. vessel uptake normalized to blood pool was 2.7 (2.0 in non-gated PET).

Bottom Line: We found increased uptake of [18F]FDG in coronary atherosclerotic lesions in a pig model.However, uptake in these early stage lesions was not detectable with in vivo PET imaging.Further studies are needed to clarify whether visible [18F]FDG uptake in coronary arteries represents more advanced, highly inflamed plaques.

View Article: PubMed Central - PubMed

Affiliation: Turku PET Centre, University of Turku and Turku University Hospital, Turku, Finland.

ABSTRACT

Objective: Inflammation is an important contributor to atherosclerosis progression. A glucose analogue 18F-fluorodeoxyglucose ([18F]FDG) has been used to detect atherosclerotic inflammation. However, it is not known to what extent [18F]FDG is taken up in different stages of atherosclerosis. We aimed to study the uptake of [18F]FDG to various stages of coronary plaques in a pig model.

Methods: First, diabetes was caused by streptozotocin injections (50 mg/kg for 3 days) in farm pigs (n = 10). After 6 months on high-fat diet, pigs underwent dual-gated cardiac PET/CT to measure [18F]FDG uptake in coronary arteries. Coronary segments (n = 33) were harvested for ex vivo measurement of radioactivity and autoradiography (ARG).

Results: Intimal thickening was observed in 16 segments and atheroma type plaques in 10 segments. Compared with the normal vessel wall, ARG showed 1.7±0.7 times higher [18F]FDG accumulation in the intimal thickening and 4.1±2.3 times higher in the atheromas (P = 0.004 and P = 0.003, respectively). Ex vivo mean vessel-to-blood ratio was higher in segments with atheroma than those without atherosclerosis (2.6±1.2 vs. 1.3±0.7, P = 0.04). In vivo PET imaging showed the highest target-to-background ratio (TBR) of 2.7. However, maximum TBR was not significantly different in segments without atherosclerosis (1.1±0.5) and either intimal thickening (1.2±0.4, P = 1.0) or atheroma (1.6±0.6, P = 0.4).

Conclusions: We found increased uptake of [18F]FDG in coronary atherosclerotic lesions in a pig model. However, uptake in these early stage lesions was not detectable with in vivo PET imaging. Further studies are needed to clarify whether visible [18F]FDG uptake in coronary arteries represents more advanced, highly inflamed plaques.

No MeSH data available.


Related in: MedlinePlus