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Methodology to study polymers interaction by surface plasmon resonance imaging.

Vollmer N, Trombini F, Hely M, Bellon S, Mercier K, Cazeneuve C - MethodsX (2014)

Bottom Line: This technique allowed carrying out parallel assays for optimizing the amount of complexes formed, the host polymer being spotted at five concentrations.It was then possible to study the influence of the concentration in host system for two concentrations of the guest polymer.The concentration in the host polymer yielding the highest immobilization of the guest system was further determined.

View Article: PubMed Central - PubMed

Affiliation: HORIBA Scientific, Avenue de la Vauve, Passage Jobin Yvon, 91120 Palaiseau Cedex, France.

ABSTRACT
The surface plasmon resonance (SPR) technique has been primarily used in the field of biology, in particular for the study of antibody-antigen interactions. Recently, polymers were introduced to form inclusion complexes. We describe here, a methodology based on surface plasmon resonance imaging to study water-resistant and reversible inclusion complexes using systems which are compatible with a cosmetic use. The purpose of this study is to follow in real time the interaction between two polymers. To carry out this study: •A biochip based on a covalent binding of one "host polymer" on a gold-activated surface was developed.•The binding of the host polymer to a guest polymer was monitored.•The presence of interactions between the β-cyclodextrins groups of the host polymer and the adamantyl functional groups of the guest polymer and the possibility of dissociating the complex were established. This technique allowed carrying out parallel assays for optimizing the amount of complexes formed, the host polymer being spotted at five concentrations. It was then possible to study the influence of the concentration in host system for two concentrations of the guest polymer. The concentration in the host polymer yielding the highest immobilization of the guest system was further determined.

No MeSH data available.


Related in: MedlinePlus

Kinetic curves and difference images after injecting the guest polymer at (A) 1.0g/L and (B) 0.1g/L. The blue curves correspond to HP/GP interactions and the red curve corresponds to the SPRi response on negative control spots. Active sites, where interaction has occurred, show up as bright spots and dark areas showing no active interaction, correspond to the control “water” sites (see map Fig. 2).
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fig0015: Kinetic curves and difference images after injecting the guest polymer at (A) 1.0g/L and (B) 0.1g/L. The blue curves correspond to HP/GP interactions and the red curve corresponds to the SPRi response on negative control spots. Active sites, where interaction has occurred, show up as bright spots and dark areas showing no active interaction, correspond to the control “water” sites (see map Fig. 2).

Mentions: The kinetic curves (Fig. 3) show the association/dissociation profiles between the host polymer immobilized on the surface of the biochip and the guest polymer injected at 1 g/L and 0.1 g/L. Each kinetic curve corresponds to an average curve of the 8 or 9 spots used for each concentration, providing a robust and accurate average value.


Methodology to study polymers interaction by surface plasmon resonance imaging.

Vollmer N, Trombini F, Hely M, Bellon S, Mercier K, Cazeneuve C - MethodsX (2014)

Kinetic curves and difference images after injecting the guest polymer at (A) 1.0g/L and (B) 0.1g/L. The blue curves correspond to HP/GP interactions and the red curve corresponds to the SPRi response on negative control spots. Active sites, where interaction has occurred, show up as bright spots and dark areas showing no active interaction, correspond to the control “water” sites (see map Fig. 2).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487328&req=5

fig0015: Kinetic curves and difference images after injecting the guest polymer at (A) 1.0g/L and (B) 0.1g/L. The blue curves correspond to HP/GP interactions and the red curve corresponds to the SPRi response on negative control spots. Active sites, where interaction has occurred, show up as bright spots and dark areas showing no active interaction, correspond to the control “water” sites (see map Fig. 2).
Mentions: The kinetic curves (Fig. 3) show the association/dissociation profiles between the host polymer immobilized on the surface of the biochip and the guest polymer injected at 1 g/L and 0.1 g/L. Each kinetic curve corresponds to an average curve of the 8 or 9 spots used for each concentration, providing a robust and accurate average value.

Bottom Line: This technique allowed carrying out parallel assays for optimizing the amount of complexes formed, the host polymer being spotted at five concentrations.It was then possible to study the influence of the concentration in host system for two concentrations of the guest polymer.The concentration in the host polymer yielding the highest immobilization of the guest system was further determined.

View Article: PubMed Central - PubMed

Affiliation: HORIBA Scientific, Avenue de la Vauve, Passage Jobin Yvon, 91120 Palaiseau Cedex, France.

ABSTRACT
The surface plasmon resonance (SPR) technique has been primarily used in the field of biology, in particular for the study of antibody-antigen interactions. Recently, polymers were introduced to form inclusion complexes. We describe here, a methodology based on surface plasmon resonance imaging to study water-resistant and reversible inclusion complexes using systems which are compatible with a cosmetic use. The purpose of this study is to follow in real time the interaction between two polymers. To carry out this study: •A biochip based on a covalent binding of one "host polymer" on a gold-activated surface was developed.•The binding of the host polymer to a guest polymer was monitored.•The presence of interactions between the β-cyclodextrins groups of the host polymer and the adamantyl functional groups of the guest polymer and the possibility of dissociating the complex were established. This technique allowed carrying out parallel assays for optimizing the amount of complexes formed, the host polymer being spotted at five concentrations. It was then possible to study the influence of the concentration in host system for two concentrations of the guest polymer. The concentration in the host polymer yielding the highest immobilization of the guest system was further determined.

No MeSH data available.


Related in: MedlinePlus