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Structure of the K12 capsule containing 5,7-di-N-acetylacinetaminic acid from Acinetobacter baumannii isolate D36.

Kenyon JJ, Marzaioli AM, Hall RM, De Castro C - Glycobiology (2015)

Bottom Line: This matched the sugar composition of the K12 capsule and the genetic content of the KL12 capsule gene cluster reported previously.D-FucpNAc was predicted to be the substrate for the initiating transferase, ItrB3, with the Wzy polymerase making a α-D-FucpNAc-(1 → 3)-D-GalpNAc linkage between the repeat units.The three glycosyltransferases encoded by KL12 are all retaining glycosyltransferases and were predicted to form specific linkages between the sugars in the K12 repeat unit.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.

No MeSH data available.


Assignment of ItrB3, Wzy and glycosyltransferases. Sugar names are abbreviated (listed in abbreviations). Square brackets mark the ends of the oligosaccharide unit, with n indicating variable numbers of repeats. Glycosyltransferases, Wzy and the ItrB3 initiating transferase are shown in bold next to the linkage they catalyze.
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CWV028F6: Assignment of ItrB3, Wzy and glycosyltransferases. Sugar names are abbreviated (listed in abbreviations). Square brackets mark the ends of the oligosaccharide unit, with n indicating variable numbers of repeats. Glycosyltransferases, Wzy and the ItrB3 initiating transferase are shown in bold next to the linkage they catalyze.

Mentions: KL12 includes three glycosyltransferase genes, gtr59, gtr30 and gtr31 (Kenyon, Marzaioli, De Castro, et al. 2015), consistent with the three internal linkages present in the K12 repeat unit (Figure 6). Two predicted glycosyltransferases, Gtr31 and Gtr30, belong to the GT4 family of retaining glycosyltransferases in the Carbohydrate Active enZYmes (CAZy) database (Lombard et al. 2014), whereas Gtr59 does not match any structure in the CAZy database and represents a novel family.Fig. 6.


Structure of the K12 capsule containing 5,7-di-N-acetylacinetaminic acid from Acinetobacter baumannii isolate D36.

Kenyon JJ, Marzaioli AM, Hall RM, De Castro C - Glycobiology (2015)

Assignment of ItrB3, Wzy and glycosyltransferases. Sugar names are abbreviated (listed in abbreviations). Square brackets mark the ends of the oligosaccharide unit, with n indicating variable numbers of repeats. Glycosyltransferases, Wzy and the ItrB3 initiating transferase are shown in bold next to the linkage they catalyze.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487303&req=5

CWV028F6: Assignment of ItrB3, Wzy and glycosyltransferases. Sugar names are abbreviated (listed in abbreviations). Square brackets mark the ends of the oligosaccharide unit, with n indicating variable numbers of repeats. Glycosyltransferases, Wzy and the ItrB3 initiating transferase are shown in bold next to the linkage they catalyze.
Mentions: KL12 includes three glycosyltransferase genes, gtr59, gtr30 and gtr31 (Kenyon, Marzaioli, De Castro, et al. 2015), consistent with the three internal linkages present in the K12 repeat unit (Figure 6). Two predicted glycosyltransferases, Gtr31 and Gtr30, belong to the GT4 family of retaining glycosyltransferases in the Carbohydrate Active enZYmes (CAZy) database (Lombard et al. 2014), whereas Gtr59 does not match any structure in the CAZy database and represents a novel family.Fig. 6.

Bottom Line: This matched the sugar composition of the K12 capsule and the genetic content of the KL12 capsule gene cluster reported previously.D-FucpNAc was predicted to be the substrate for the initiating transferase, ItrB3, with the Wzy polymerase making a α-D-FucpNAc-(1 → 3)-D-GalpNAc linkage between the repeat units.The three glycosyltransferases encoded by KL12 are all retaining glycosyltransferases and were predicted to form specific linkages between the sugars in the K12 repeat unit.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.

No MeSH data available.