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Structure of the K12 capsule containing 5,7-di-N-acetylacinetaminic acid from Acinetobacter baumannii isolate D36.

Kenyon JJ, Marzaioli AM, Hall RM, De Castro C - Glycobiology (2015)

Bottom Line: This matched the sugar composition of the K12 capsule and the genetic content of the KL12 capsule gene cluster reported previously.D-FucpNAc was predicted to be the substrate for the initiating transferase, ItrB3, with the Wzy polymerase making a α-D-FucpNAc-(1 → 3)-D-GalpNAc linkage between the repeat units.The three glycosyltransferases encoded by KL12 are all retaining glycosyltransferases and were predicted to form specific linkages between the sugars in the K12 repeat unit.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.

No MeSH data available.


KL12 capsule gene cluster. Figure is drawn to scale from GenBank accession number JN107991. KL12 was reported previously in Kenyon, Marzaioli, De Castro (2015). Genes are arrows that indicate direction of transcription, and their names are shown above. Genes for capsule export are indicated on the left. Horizontal bars above the gene cluster indicate genes that direct the synthesis of nucleotide-linked sugars, with sugar names shown above. Glycosyltransferase genes are gray, and the initiating transferase gene is black. Striped genes are those encoding products that have no assigned function for the synthesis of K12.
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CWV028F1: KL12 capsule gene cluster. Figure is drawn to scale from GenBank accession number JN107991. KL12 was reported previously in Kenyon, Marzaioli, De Castro (2015). Genes are arrows that indicate direction of transcription, and their names are shown above. Genes for capsule export are indicated on the left. Horizontal bars above the gene cluster indicate genes that direct the synthesis of nucleotide-linked sugars, with sugar names shown above. Glycosyltransferase genes are gray, and the initiating transferase gene is black. Striped genes are those encoding products that have no assigned function for the synthesis of K12.

Mentions: We recently reported the sugar composition of the K12 capsule from an extensively antibiotic resistant A. baumannii GC1 isolate (Kenyon, Marzaioli, De Castro, et al. 2015). K12 includes a novel non-2-ulosonic acid, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-altro-non-2-ulosonic acid, that had not previously been identified in a biological sample. We named this sugar 5,7-di-N-acetylacinetaminic acid (Aci5Ac7Ac). In the KL12 gene cluster (GenBank accession number JN107991), 10 genes would direct the synthesis of CMP-α-Aci5Ac7Ac (Figure 1). The K12 capsule also includes N-acetyl-d-galactosamine (d-GalpNAc), N-acetyl-l-fucosamine (l-FucpNAc) and N-acetyl-d-fucosamine (d-FucpNAc). These findings were also consistent with the genetic content of the KL12 gene cluster that contains genes previously predicted to be responsible for the synthesis of UDP-l-FucpNAc (fnlABC) and UDP-D-FucpNAc (fnr1 and gdr). The gne1 gene responsible for the conversion of the simple sugar UDP-d-GlcpNAc to UDP-D-GalpNAc is found in KL12 and in most Acinetobacter capsule biosynthesis gene clusters (Hu et al. 2013; Kenyon and Hall 2013).Fig. 1.


Structure of the K12 capsule containing 5,7-di-N-acetylacinetaminic acid from Acinetobacter baumannii isolate D36.

Kenyon JJ, Marzaioli AM, Hall RM, De Castro C - Glycobiology (2015)

KL12 capsule gene cluster. Figure is drawn to scale from GenBank accession number JN107991. KL12 was reported previously in Kenyon, Marzaioli, De Castro (2015). Genes are arrows that indicate direction of transcription, and their names are shown above. Genes for capsule export are indicated on the left. Horizontal bars above the gene cluster indicate genes that direct the synthesis of nucleotide-linked sugars, with sugar names shown above. Glycosyltransferase genes are gray, and the initiating transferase gene is black. Striped genes are those encoding products that have no assigned function for the synthesis of K12.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4487303&req=5

CWV028F1: KL12 capsule gene cluster. Figure is drawn to scale from GenBank accession number JN107991. KL12 was reported previously in Kenyon, Marzaioli, De Castro (2015). Genes are arrows that indicate direction of transcription, and their names are shown above. Genes for capsule export are indicated on the left. Horizontal bars above the gene cluster indicate genes that direct the synthesis of nucleotide-linked sugars, with sugar names shown above. Glycosyltransferase genes are gray, and the initiating transferase gene is black. Striped genes are those encoding products that have no assigned function for the synthesis of K12.
Mentions: We recently reported the sugar composition of the K12 capsule from an extensively antibiotic resistant A. baumannii GC1 isolate (Kenyon, Marzaioli, De Castro, et al. 2015). K12 includes a novel non-2-ulosonic acid, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-altro-non-2-ulosonic acid, that had not previously been identified in a biological sample. We named this sugar 5,7-di-N-acetylacinetaminic acid (Aci5Ac7Ac). In the KL12 gene cluster (GenBank accession number JN107991), 10 genes would direct the synthesis of CMP-α-Aci5Ac7Ac (Figure 1). The K12 capsule also includes N-acetyl-d-galactosamine (d-GalpNAc), N-acetyl-l-fucosamine (l-FucpNAc) and N-acetyl-d-fucosamine (d-FucpNAc). These findings were also consistent with the genetic content of the KL12 gene cluster that contains genes previously predicted to be responsible for the synthesis of UDP-l-FucpNAc (fnlABC) and UDP-D-FucpNAc (fnr1 and gdr). The gne1 gene responsible for the conversion of the simple sugar UDP-d-GlcpNAc to UDP-D-GalpNAc is found in KL12 and in most Acinetobacter capsule biosynthesis gene clusters (Hu et al. 2013; Kenyon and Hall 2013).Fig. 1.

Bottom Line: This matched the sugar composition of the K12 capsule and the genetic content of the KL12 capsule gene cluster reported previously.D-FucpNAc was predicted to be the substrate for the initiating transferase, ItrB3, with the Wzy polymerase making a α-D-FucpNAc-(1 → 3)-D-GalpNAc linkage between the repeat units.The three glycosyltransferases encoded by KL12 are all retaining glycosyltransferases and were predicted to form specific linkages between the sugars in the K12 repeat unit.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia.

No MeSH data available.