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Understanding Heterogeneity and Permeability of Brain Metastases in Murine Models of HER2-Positive Breast Cancer Through Magnetic Resonance Imaging: Implications for Detection and Therapy.

Murrell DH, Hamilton AM, Mallett CL, van Gorkum R, Chambers AF, Foster PJ - Transl Oncol (2015)

Bottom Line: The mean volume of an MDA-MB-231-BR-HER2 tumor was significantly larger compared to other models (F2,12 = 5.845, P < .05); interestingly, this model also had a significantly higher proportion of Gd-impermeable tumors (F2,12 = 22.18, P < .0001).Ki67 staining indicated that Gd-impermeable tumors had significantly more proliferative nuclei compared to Gd-permeable tumors (t[24] = 2.389, P < .05) in the MDA-MB-231-BR-HER2 model.Understanding this heterogeneity, especially as it relates to BBB permeability, is important for improvement in brain metastasis detection and treatment delivery.

View Article: PubMed Central - PubMed

Affiliation: Imaging, Robarts Research Institute, London, Ontario, Canada; Medical Biophysics, Western University, London, Ontario, Canada. Electronic address: dmurrell@robarts.ca.

No MeSH data available.


Related in: MedlinePlus

Representative images showing metastases resulting from SUM190-BR3, JIMT-1-BR3, or MDA-MB-231-BR-HER2 human brain metastatic breast cancer. (A-C) bSSFP images show metastasis burden (hyperintensities); (D-F) correlative H&E-stained sections of the area indicated by boxes show morphology of tumors in each model; (G-I) Ki67 staining indicates proliferative nuclei in brown. The region of signal void in the center of the SUM190-BR3 tumor (A) relates to the necrotic core seen in histology (D). JIMT-1-BR3 tumors are hyperintense in bSSFP (B) and grow as dense and proliferative nuclei clusters (E, H). 231-BR-HER2 tumors are the most hyperintense in bSSFP (C) and present as clusters of highly proliferative cell nuclei within pockets of edema in H&E (1, I).
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f0005: Representative images showing metastases resulting from SUM190-BR3, JIMT-1-BR3, or MDA-MB-231-BR-HER2 human brain metastatic breast cancer. (A-C) bSSFP images show metastasis burden (hyperintensities); (D-F) correlative H&E-stained sections of the area indicated by boxes show morphology of tumors in each model; (G-I) Ki67 staining indicates proliferative nuclei in brown. The region of signal void in the center of the SUM190-BR3 tumor (A) relates to the necrotic core seen in histology (D). JIMT-1-BR3 tumors are hyperintense in bSSFP (B) and grow as dense and proliferative nuclei clusters (E, H). 231-BR-HER2 tumors are the most hyperintense in bSSFP (C) and present as clusters of highly proliferative cell nuclei within pockets of edema in H&E (1, I).

Mentions: Representative images of brain metastases from each group are shown in Figure 1. Contrast in bSSFP images is related to the ratio of spin–spin to spin–lattice relaxations (T2/T1); in general, metastases appear hyperintense compared to brain parenchyma because of relatively higher fluid content and therefore a longer T2 and T2/T1 ratio.


Understanding Heterogeneity and Permeability of Brain Metastases in Murine Models of HER2-Positive Breast Cancer Through Magnetic Resonance Imaging: Implications for Detection and Therapy.

Murrell DH, Hamilton AM, Mallett CL, van Gorkum R, Chambers AF, Foster PJ - Transl Oncol (2015)

Representative images showing metastases resulting from SUM190-BR3, JIMT-1-BR3, or MDA-MB-231-BR-HER2 human brain metastatic breast cancer. (A-C) bSSFP images show metastasis burden (hyperintensities); (D-F) correlative H&E-stained sections of the area indicated by boxes show morphology of tumors in each model; (G-I) Ki67 staining indicates proliferative nuclei in brown. The region of signal void in the center of the SUM190-BR3 tumor (A) relates to the necrotic core seen in histology (D). JIMT-1-BR3 tumors are hyperintense in bSSFP (B) and grow as dense and proliferative nuclei clusters (E, H). 231-BR-HER2 tumors are the most hyperintense in bSSFP (C) and present as clusters of highly proliferative cell nuclei within pockets of edema in H&E (1, I).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487267&req=5

f0005: Representative images showing metastases resulting from SUM190-BR3, JIMT-1-BR3, or MDA-MB-231-BR-HER2 human brain metastatic breast cancer. (A-C) bSSFP images show metastasis burden (hyperintensities); (D-F) correlative H&E-stained sections of the area indicated by boxes show morphology of tumors in each model; (G-I) Ki67 staining indicates proliferative nuclei in brown. The region of signal void in the center of the SUM190-BR3 tumor (A) relates to the necrotic core seen in histology (D). JIMT-1-BR3 tumors are hyperintense in bSSFP (B) and grow as dense and proliferative nuclei clusters (E, H). 231-BR-HER2 tumors are the most hyperintense in bSSFP (C) and present as clusters of highly proliferative cell nuclei within pockets of edema in H&E (1, I).
Mentions: Representative images of brain metastases from each group are shown in Figure 1. Contrast in bSSFP images is related to the ratio of spin–spin to spin–lattice relaxations (T2/T1); in general, metastases appear hyperintense compared to brain parenchyma because of relatively higher fluid content and therefore a longer T2 and T2/T1 ratio.

Bottom Line: The mean volume of an MDA-MB-231-BR-HER2 tumor was significantly larger compared to other models (F2,12 = 5.845, P < .05); interestingly, this model also had a significantly higher proportion of Gd-impermeable tumors (F2,12 = 22.18, P < .0001).Ki67 staining indicated that Gd-impermeable tumors had significantly more proliferative nuclei compared to Gd-permeable tumors (t[24] = 2.389, P < .05) in the MDA-MB-231-BR-HER2 model.Understanding this heterogeneity, especially as it relates to BBB permeability, is important for improvement in brain metastasis detection and treatment delivery.

View Article: PubMed Central - PubMed

Affiliation: Imaging, Robarts Research Institute, London, Ontario, Canada; Medical Biophysics, Western University, London, Ontario, Canada. Electronic address: dmurrell@robarts.ca.

No MeSH data available.


Related in: MedlinePlus