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Dual Action of Myricetin on Porphyromonas gingivalis and the Inflammatory Response of Host Cells: A Promising Therapeutic Molecule for Periodontal Diseases.

Grenier D, Chen H, Ben Lagha A, Fournier-Larente J, Morin MP - PLoS ONE (2015)

Bottom Line: Myricetin was found to attenuate the virulence of P. gingivalis by reducing the expression of genes coding for important virulence factors, including proteinases (rgpA, rgpB, and kgp) and adhesins (fimA, hagA, and hagB).In conclusion, our study brought clear evidence that the flavonol myricetin exhibits a dual action on the periodontopathogenic bacterium P. gingivalis and the inflammatory response of host cells.Therefore, myricetin holds promise as a therapeutic agent for the treatment/prevention of periodontitis.

View Article: PubMed Central - PubMed

Affiliation: Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, Quebec, Canada.

ABSTRACT
Periodontitis that affects the underlying structures of the periodontium, including the alveolar bone, is a multifactorial disease, whose etiology involves interactions between specific bacterial species of the subgingival biofilm and the host immune components. In the present study, we investigated the effects of myricetin, a flavonol largely distributed in fruits and vegetables, on growth and virulence properties of Porphyromonas gingivalis as well as on the P. gingivalis-induced inflammatory response in host cells. Minimal inhibitory concentration values of myricetin against P. gingivalis were in the range of 62.5 to 125 μg/ml. The iron-chelating activity of myricetin may contribute to the antibacterial activity of this flavonol. Myricetin was found to attenuate the virulence of P. gingivalis by reducing the expression of genes coding for important virulence factors, including proteinases (rgpA, rgpB, and kgp) and adhesins (fimA, hagA, and hagB). Myricetin dose-dependently prevented NF-κB activation in a monocyte model. Moreover, it inhibited the secretion of IL-6, IL-8 and MMP-3 by P. gingivalis-stimulated gingival fibroblasts. In conclusion, our study brought clear evidence that the flavonol myricetin exhibits a dual action on the periodontopathogenic bacterium P. gingivalis and the inflammatory response of host cells. Therefore, myricetin holds promise as a therapeutic agent for the treatment/prevention of periodontitis.

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Related in: MedlinePlus

Iron-chelating activity of myricetin as determined by the universal siderophore colorimetric assay using chrome azurol sulfate.Reduction of A630 occurs when a strong chelator removes the iron from the dye chrome azurol sulfate. Ferrichrome, a siderophore produced by U. sphaerogena, was used as positive control.
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pone.0131758.g001: Iron-chelating activity of myricetin as determined by the universal siderophore colorimetric assay using chrome azurol sulfate.Reduction of A630 occurs when a strong chelator removes the iron from the dye chrome azurol sulfate. Ferrichrome, a siderophore produced by U. sphaerogena, was used as positive control.

Mentions: A preliminary assay revealed that myricetin did not possess membrane permeabilizing activity on P. gingivalis (data not shown) that may have been involved in its antibacterial property. In an attempt to identify the mechanism by which myricetin exhibits antibacterial activity against P. gingivalis, we investigated whether this flavonol possesses a siderophore activity resulting in the chelation of iron, an essential element for most bacteria. Data presented in Fig 1 indicate that myricetin dose-dependently chelates iron in a universal siderophore assay using chrome azurol sulfate. The activity reached a maximum at a concentration of myricetin ≥ 50 μg/ml. The iron-chelating activity of myricetin was stronger than that observed with ferrichrome, a reference siderophore produced by U. sphaerogena.


Dual Action of Myricetin on Porphyromonas gingivalis and the Inflammatory Response of Host Cells: A Promising Therapeutic Molecule for Periodontal Diseases.

Grenier D, Chen H, Ben Lagha A, Fournier-Larente J, Morin MP - PLoS ONE (2015)

Iron-chelating activity of myricetin as determined by the universal siderophore colorimetric assay using chrome azurol sulfate.Reduction of A630 occurs when a strong chelator removes the iron from the dye chrome azurol sulfate. Ferrichrome, a siderophore produced by U. sphaerogena, was used as positive control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487256&req=5

pone.0131758.g001: Iron-chelating activity of myricetin as determined by the universal siderophore colorimetric assay using chrome azurol sulfate.Reduction of A630 occurs when a strong chelator removes the iron from the dye chrome azurol sulfate. Ferrichrome, a siderophore produced by U. sphaerogena, was used as positive control.
Mentions: A preliminary assay revealed that myricetin did not possess membrane permeabilizing activity on P. gingivalis (data not shown) that may have been involved in its antibacterial property. In an attempt to identify the mechanism by which myricetin exhibits antibacterial activity against P. gingivalis, we investigated whether this flavonol possesses a siderophore activity resulting in the chelation of iron, an essential element for most bacteria. Data presented in Fig 1 indicate that myricetin dose-dependently chelates iron in a universal siderophore assay using chrome azurol sulfate. The activity reached a maximum at a concentration of myricetin ≥ 50 μg/ml. The iron-chelating activity of myricetin was stronger than that observed with ferrichrome, a reference siderophore produced by U. sphaerogena.

Bottom Line: Myricetin was found to attenuate the virulence of P. gingivalis by reducing the expression of genes coding for important virulence factors, including proteinases (rgpA, rgpB, and kgp) and adhesins (fimA, hagA, and hagB).In conclusion, our study brought clear evidence that the flavonol myricetin exhibits a dual action on the periodontopathogenic bacterium P. gingivalis and the inflammatory response of host cells.Therefore, myricetin holds promise as a therapeutic agent for the treatment/prevention of periodontitis.

View Article: PubMed Central - PubMed

Affiliation: Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, Quebec, Canada.

ABSTRACT
Periodontitis that affects the underlying structures of the periodontium, including the alveolar bone, is a multifactorial disease, whose etiology involves interactions between specific bacterial species of the subgingival biofilm and the host immune components. In the present study, we investigated the effects of myricetin, a flavonol largely distributed in fruits and vegetables, on growth and virulence properties of Porphyromonas gingivalis as well as on the P. gingivalis-induced inflammatory response in host cells. Minimal inhibitory concentration values of myricetin against P. gingivalis were in the range of 62.5 to 125 μg/ml. The iron-chelating activity of myricetin may contribute to the antibacterial activity of this flavonol. Myricetin was found to attenuate the virulence of P. gingivalis by reducing the expression of genes coding for important virulence factors, including proteinases (rgpA, rgpB, and kgp) and adhesins (fimA, hagA, and hagB). Myricetin dose-dependently prevented NF-κB activation in a monocyte model. Moreover, it inhibited the secretion of IL-6, IL-8 and MMP-3 by P. gingivalis-stimulated gingival fibroblasts. In conclusion, our study brought clear evidence that the flavonol myricetin exhibits a dual action on the periodontopathogenic bacterium P. gingivalis and the inflammatory response of host cells. Therefore, myricetin holds promise as a therapeutic agent for the treatment/prevention of periodontitis.

No MeSH data available.


Related in: MedlinePlus