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Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Related in: MedlinePlus

The nanocapsules contained p53 and paclitaxel, which can synergistically induced cell apoptosis.Notes: (A) Growth inhibition rate (%) of Hela cells after treatment with nanocapsules of p53 (50 μg/mL), paclitaxel (50 μg/mL), or both of them. The data were expressed as mean ± SEM (n%4). (B) Apoptotic cells treated with nanocapsules containing p53 and paclitaxel observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviation: SEM, standard error of mean.
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f6-ijn-10-4149: The nanocapsules contained p53 and paclitaxel, which can synergistically induced cell apoptosis.Notes: (A) Growth inhibition rate (%) of Hela cells after treatment with nanocapsules of p53 (50 μg/mL), paclitaxel (50 μg/mL), or both of them. The data were expressed as mean ± SEM (n%4). (B) Apoptotic cells treated with nanocapsules containing p53 and paclitaxel observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviation: SEM, standard error of mean.

Mentions: To examine whether the system could be used to deliver drugs into cells for theranostics, the nanocapsules containing p53 and paclitaxel30,31 were prepared; paclitaxel was dissolved in the oil phase (loading amount of 50 μg/mL) and the wild-type p53 (loading amount of 50 μg/mL) was packed into the inner aqueous phase. After 24 hours of incubation, Hela cells showed the features of cell apoptosis. Under the fluorescence microscope, green fluorescence was observed due to the fluorescence of FITC–BSA on the surface of the nanocapsule shell. Compared with the p53 or paclitaxel nanocapsule at each concentration, the synergistic anticancer effect was detected obviously by cell viability assay (Figure 6), suggesting that the system could simultaneously deliver lipophilic drugs and hydrophilic proteins into cells for theranostics.


Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

The nanocapsules contained p53 and paclitaxel, which can synergistically induced cell apoptosis.Notes: (A) Growth inhibition rate (%) of Hela cells after treatment with nanocapsules of p53 (50 μg/mL), paclitaxel (50 μg/mL), or both of them. The data were expressed as mean ± SEM (n%4). (B) Apoptotic cells treated with nanocapsules containing p53 and paclitaxel observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviation: SEM, standard error of mean.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487237&req=5

f6-ijn-10-4149: The nanocapsules contained p53 and paclitaxel, which can synergistically induced cell apoptosis.Notes: (A) Growth inhibition rate (%) of Hela cells after treatment with nanocapsules of p53 (50 μg/mL), paclitaxel (50 μg/mL), or both of them. The data were expressed as mean ± SEM (n%4). (B) Apoptotic cells treated with nanocapsules containing p53 and paclitaxel observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviation: SEM, standard error of mean.
Mentions: To examine whether the system could be used to deliver drugs into cells for theranostics, the nanocapsules containing p53 and paclitaxel30,31 were prepared; paclitaxel was dissolved in the oil phase (loading amount of 50 μg/mL) and the wild-type p53 (loading amount of 50 μg/mL) was packed into the inner aqueous phase. After 24 hours of incubation, Hela cells showed the features of cell apoptosis. Under the fluorescence microscope, green fluorescence was observed due to the fluorescence of FITC–BSA on the surface of the nanocapsule shell. Compared with the p53 or paclitaxel nanocapsule at each concentration, the synergistic anticancer effect was detected obviously by cell viability assay (Figure 6), suggesting that the system could simultaneously deliver lipophilic drugs and hydrophilic proteins into cells for theranostics.

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Related in: MedlinePlus