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Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Related in: MedlinePlus

The nanocapsules containing p53-induced cell apoptosis.Notes: (A) p53 protein expression analyzed by SDS–PAGE. M, protein molecular weight markers; Lane 1, uninduced Escherichia coli harboring pET-28a/p53; Lane 2, the supernatant; Lane 3, the precipitate of induced E. coli harboring pET-28a/p53; Lane 4, purified p53. (B) Growth inhibition rate (%) of Hela cells after treatment with different concentrations of p53 or p53 nanocapsules. The inhibition rate (%) was calculated as the value of (sample – blank)/(control – blank). The cultured cells without any treatment were used as the control and the culture well without cells as the blank. The data were expressed as mean ± SEM (n%4). (C) Hela cells were treated with 50 μg/mL p53 or p53 nanocapsules and observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviations: SDA–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SEM, standard error of mean.
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f5-ijn-10-4149: The nanocapsules containing p53-induced cell apoptosis.Notes: (A) p53 protein expression analyzed by SDS–PAGE. M, protein molecular weight markers; Lane 1, uninduced Escherichia coli harboring pET-28a/p53; Lane 2, the supernatant; Lane 3, the precipitate of induced E. coli harboring pET-28a/p53; Lane 4, purified p53. (B) Growth inhibition rate (%) of Hela cells after treatment with different concentrations of p53 or p53 nanocapsules. The inhibition rate (%) was calculated as the value of (sample – blank)/(control – blank). The cultured cells without any treatment were used as the control and the culture well without cells as the blank. The data were expressed as mean ± SEM (n%4). (C) Hela cells were treated with 50 μg/mL p53 or p53 nanocapsules and observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviations: SDA–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SEM, standard error of mean.

Mentions: The nanocapsules containing wild-type p53 (Figure 5A) were prepared by encapsulating p53 into the inner aqueous phase, and FITC–BSA was used as shell. The results showed that the nanocapsule containing different concentrations of p53 (2.5–50 μg/mL) inhibited Hela cell growth in a concentration-dependent manner after 24 hours of incubation, which showed a significantly higher cell inhibition rate than the corresponding amount of p53 alone (Figure 5B). Additionally, the cells treated with p53 nanocapsules showed similar morphologic changes, including cell shrinkage, chromatin condensation, and the presence of “apoptosis bodies” under the optical microscope, as well as displaying obvious fluorescence under the fluorescence microscope (Figure 5C).


Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

The nanocapsules containing p53-induced cell apoptosis.Notes: (A) p53 protein expression analyzed by SDS–PAGE. M, protein molecular weight markers; Lane 1, uninduced Escherichia coli harboring pET-28a/p53; Lane 2, the supernatant; Lane 3, the precipitate of induced E. coli harboring pET-28a/p53; Lane 4, purified p53. (B) Growth inhibition rate (%) of Hela cells after treatment with different concentrations of p53 or p53 nanocapsules. The inhibition rate (%) was calculated as the value of (sample – blank)/(control – blank). The cultured cells without any treatment were used as the control and the culture well without cells as the blank. The data were expressed as mean ± SEM (n%4). (C) Hela cells were treated with 50 μg/mL p53 or p53 nanocapsules and observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviations: SDA–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SEM, standard error of mean.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4487237&req=5

f5-ijn-10-4149: The nanocapsules containing p53-induced cell apoptosis.Notes: (A) p53 protein expression analyzed by SDS–PAGE. M, protein molecular weight markers; Lane 1, uninduced Escherichia coli harboring pET-28a/p53; Lane 2, the supernatant; Lane 3, the precipitate of induced E. coli harboring pET-28a/p53; Lane 4, purified p53. (B) Growth inhibition rate (%) of Hela cells after treatment with different concentrations of p53 or p53 nanocapsules. The inhibition rate (%) was calculated as the value of (sample – blank)/(control – blank). The cultured cells without any treatment were used as the control and the culture well without cells as the blank. The data were expressed as mean ± SEM (n%4). (C) Hela cells were treated with 50 μg/mL p53 or p53 nanocapsules and observed under optical and fluorescence microscope. Scale bar 20 μm.Abbreviations: SDA–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SEM, standard error of mean.
Mentions: The nanocapsules containing wild-type p53 (Figure 5A) were prepared by encapsulating p53 into the inner aqueous phase, and FITC–BSA was used as shell. The results showed that the nanocapsule containing different concentrations of p53 (2.5–50 μg/mL) inhibited Hela cell growth in a concentration-dependent manner after 24 hours of incubation, which showed a significantly higher cell inhibition rate than the corresponding amount of p53 alone (Figure 5B). Additionally, the cells treated with p53 nanocapsules showed similar morphologic changes, including cell shrinkage, chromatin condensation, and the presence of “apoptosis bodies” under the optical microscope, as well as displaying obvious fluorescence under the fluorescence microscope (Figure 5C).

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Related in: MedlinePlus