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Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Related in: MedlinePlus

Fluorescence intensities and cytotoxicity of nanocapsules in Hela cells.Notes: Fluorescence intensities of green (A) and red (B) of cells increased along with the increment of nanocapsule concentrations. FITC–BSA and TRITC–BSA were used as controls, respectively. (C) Cytotoxicity of the nanocapsules with different concentrations by AlamarBlue cell viability assay. The data were expressed as mean ± SEM (n%4).Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin; SEM, standard error of mean.
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f3-ijn-10-4149: Fluorescence intensities and cytotoxicity of nanocapsules in Hela cells.Notes: Fluorescence intensities of green (A) and red (B) of cells increased along with the increment of nanocapsule concentrations. FITC–BSA and TRITC–BSA were used as controls, respectively. (C) Cytotoxicity of the nanocapsules with different concentrations by AlamarBlue cell viability assay. The data were expressed as mean ± SEM (n%4).Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin; SEM, standard error of mean.

Mentions: Cell transduction efficiency was determined by examining the relationship between concentration and fluorescence intensity. Cells transduced with the nanocapsules displayed strong fluorescence intensities that were closely dependent on the concentrations (Figure 3A and B). However, at the same protein concentrations, cells incubated with the controls of FITC–BSA and TRITC–BSA did not show any fluorescence.


Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

Fluorescence intensities and cytotoxicity of nanocapsules in Hela cells.Notes: Fluorescence intensities of green (A) and red (B) of cells increased along with the increment of nanocapsule concentrations. FITC–BSA and TRITC–BSA were used as controls, respectively. (C) Cytotoxicity of the nanocapsules with different concentrations by AlamarBlue cell viability assay. The data were expressed as mean ± SEM (n%4).Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin; SEM, standard error of mean.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487237&req=5

f3-ijn-10-4149: Fluorescence intensities and cytotoxicity of nanocapsules in Hela cells.Notes: Fluorescence intensities of green (A) and red (B) of cells increased along with the increment of nanocapsule concentrations. FITC–BSA and TRITC–BSA were used as controls, respectively. (C) Cytotoxicity of the nanocapsules with different concentrations by AlamarBlue cell viability assay. The data were expressed as mean ± SEM (n%4).Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin; SEM, standard error of mean.
Mentions: Cell transduction efficiency was determined by examining the relationship between concentration and fluorescence intensity. Cells transduced with the nanocapsules displayed strong fluorescence intensities that were closely dependent on the concentrations (Figure 3A and B). However, at the same protein concentrations, cells incubated with the controls of FITC–BSA and TRITC–BSA did not show any fluorescence.

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Related in: MedlinePlus