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Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.


Cell uptake of the nanocapsules in Hela cells.Notes: (A) Cell uptake process of the nanocapsules consisted of FITC–BSA coating and TRITC–BSA core. (B) Fluorescence images under confocal microscope after 60 minutes of incubation. Nuclei were stained with Hoechst 33258 (blue). Scale bar 20 μm.Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin.
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f2-ijn-10-4149: Cell uptake of the nanocapsules in Hela cells.Notes: (A) Cell uptake process of the nanocapsules consisted of FITC–BSA coating and TRITC–BSA core. (B) Fluorescence images under confocal microscope after 60 minutes of incubation. Nuclei were stained with Hoechst 33258 (blue). Scale bar 20 μm.Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin.

Mentions: The results of cellular uptake showed that after addition of the nanocapsules into cell media for 15 minutes, green and red fluorescence were captured on the cell membranes, indicating that the nanocapsules attached to the cell membranes. Subsequently, the membrane fluorescence gradually decreased, and fluorescence spots were detected in the cytosol (Figure 2A), suggesting that the nanocapsules migrated from the membranes to the cytosol. Following 60 minutes of incubation, the images showed a homogeneous distribution of the fluorescence within the cytosol (Figure 2A and B).


Biodegradable double nanocapsule as a novel multifunctional carrier for drug delivery and cell imaging.

Qian K, Wu J, Zhang E, Zhang Y, Fu A - Int J Nanomedicine (2015)

Cell uptake of the nanocapsules in Hela cells.Notes: (A) Cell uptake process of the nanocapsules consisted of FITC–BSA coating and TRITC–BSA core. (B) Fluorescence images under confocal microscope after 60 minutes of incubation. Nuclei were stained with Hoechst 33258 (blue). Scale bar 20 μm.Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4487237&req=5

f2-ijn-10-4149: Cell uptake of the nanocapsules in Hela cells.Notes: (A) Cell uptake process of the nanocapsules consisted of FITC–BSA coating and TRITC–BSA core. (B) Fluorescence images under confocal microscope after 60 minutes of incubation. Nuclei were stained with Hoechst 33258 (blue). Scale bar 20 μm.Abbreviations: FITC–BSA, fluorescein isothiocyanate–bovine serum albumin; TRITC–BSA, tetramethylrhodamine–bovine serum albumin.
Mentions: The results of cellular uptake showed that after addition of the nanocapsules into cell media for 15 minutes, green and red fluorescence were captured on the cell membranes, indicating that the nanocapsules attached to the cell membranes. Subsequently, the membrane fluorescence gradually decreased, and fluorescence spots were detected in the cytosol (Figure 2A), suggesting that the nanocapsules migrated from the membranes to the cytosol. Following 60 minutes of incubation, the images showed a homogeneous distribution of the fluorescence within the cytosol (Figure 2A and B).

Bottom Line: The nanocapsules were spherical in shape, with an average size of about 180 nm.Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope.Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, People's Republic of China ; College of Plant Protection, Southwest University, Chongqing, People's Republic of China.

ABSTRACT
Highly-efficient delivery of macromolecules into cells for both imaging and therapy (theranostics) remains a challenge for the design of a delivery system. Here, we suggested a novel hybrid protein-lipid polymer nanocapsule as an effective and nontoxic drug delivery and imaging carrier. The biodegradable nanocapsules showed the typical double emulsion features, including fluorescently labeled bovine serum albumin shell, oil phase containing poly(lactic-co-glycolic acid) and linoleic acid, and inner aqueous phase. The nanocapsules were spherical in shape, with an average size of about 180 nm. Proteins packed into the inner aqueous phase of the nanocapsules could be delivered into cells with high efficiency, and the fluorescence of the fluorescently labeled bovine serum albumin could be used for tracing the protein migration and cellular location. Further studies suggested that the co-delivery of transcription factor p53 and lipophilic drug paclitaxel with the nanocapsules acted synergistically to induce Hela cell apoptosis, and the fluorescence of apoptotic cells was clearly observed under a fluorescence microscope. Such multifunctional delivery system would have great potential applications in drug delivery and theranostic fields.

No MeSH data available.