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Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

Jin F, Zhang R, Feng S, Yuan CT, Zhang RY, Han GK, Li GH, Yu XZ, Liu Y, Kong LS, Zhang SL, Zhao L - Cancer Cell Int. (2015)

Bottom Line: HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.

ABSTRACT

Background: This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

Methods: CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.

Results: The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.

Conclusions: Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.

No MeSH data available.


Related in: MedlinePlus

GFAP staining (×40). a The positive cells of GFAP staining in TJ905 cell group presented brown; b The positive cells of GFAP staining in TJ905 stem cell group also showed brown but were more than the cells in TJ905 cell group; c Skeletomuscular tissue was shown in normal control group; d The mice in TJ905 CD133- cell group showed similar manifestation to that in TJ905 cell group
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Fig3: GFAP staining (×40). a The positive cells of GFAP staining in TJ905 cell group presented brown; b The positive cells of GFAP staining in TJ905 stem cell group also showed brown but were more than the cells in TJ905 cell group; c Skeletomuscular tissue was shown in normal control group; d The mice in TJ905 CD133- cell group showed similar manifestation to that in TJ905 cell group

Mentions: GFAP staining in TJ905 cell, TJ905 CD133- and stem cell group were positive, showing buffy or brown. The positive rate in TJ905 stem cell group was significantly higher than that in TJ905 cell and CD133- group (P < 0.05) (Fig. 3).Fig. 3


Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

Jin F, Zhang R, Feng S, Yuan CT, Zhang RY, Han GK, Li GH, Yu XZ, Liu Y, Kong LS, Zhang SL, Zhao L - Cancer Cell Int. (2015)

GFAP staining (×40). a The positive cells of GFAP staining in TJ905 cell group presented brown; b The positive cells of GFAP staining in TJ905 stem cell group also showed brown but were more than the cells in TJ905 cell group; c Skeletomuscular tissue was shown in normal control group; d The mice in TJ905 CD133- cell group showed similar manifestation to that in TJ905 cell group
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4487198&req=5

Fig3: GFAP staining (×40). a The positive cells of GFAP staining in TJ905 cell group presented brown; b The positive cells of GFAP staining in TJ905 stem cell group also showed brown but were more than the cells in TJ905 cell group; c Skeletomuscular tissue was shown in normal control group; d The mice in TJ905 CD133- cell group showed similar manifestation to that in TJ905 cell group
Mentions: GFAP staining in TJ905 cell, TJ905 CD133- and stem cell group were positive, showing buffy or brown. The positive rate in TJ905 stem cell group was significantly higher than that in TJ905 cell and CD133- group (P < 0.05) (Fig. 3).Fig. 3

Bottom Line: HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining, Shandong 272029 PR China.

ABSTRACT

Background: This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

Methods: CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.

Results: The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.

Conclusions: Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.

No MeSH data available.


Related in: MedlinePlus