Limits...
Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study.

Lee HJ, Lee J, Lee JT, Hong JS, Lim BS, Park HJ, Kim YK, Kim TI - J Periodontal Implant Sci (2015)

Bottom Line: Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs.After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens.Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 µm).

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.

ABSTRACT

Purpose: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and tissues.

Methods: Three types of commercially pure titanium discs were prepared; machined-surface discs (A), sandblasted, large-grit, acid-etched (SLA)-treated discs (B), SLA and microgroove-formed discs (C). After surface topography of the discs was examined by confocal laser scanning electron microscopy, water contact angle and surface energy were measured. Human gingival fibroblasts (hGFs) and murine osteoblastic cells (MC3T3-E1) were seeded onto the titanium discs for immunofluorescence assay of adhesion proteins. Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs. After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens.

Results: Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 µm). The expression of adhesion molecules of hGFs and MC3T30E1 cells was prominent in group C. Titanium implants with microgrooves on the coronal portion showed firm adhesion to peri-implant soft tissue.

Conclusions: Microgrooves on the titanium surface promoted the adhesion of gingival fibroblasts and osteoblastic cells, as well as favorable peri-implant soft tissue sealing.

No MeSH data available.


Related in: MedlinePlus

Laser scanning confocal microscopic images of titanium discs. A, machined-surface discs; B, sandblasted, large-grit, acid-etched (SLA)-treated discs; C, SLA-treated and microgroove-formed discs. Bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4485062&req=5

Figure 2: Laser scanning confocal microscopic images of titanium discs. A, machined-surface discs; B, sandblasted, large-grit, acid-etched (SLA)-treated discs; C, SLA-treated and microgroove-formed discs. Bar=100 µm.

Mentions: Each group of titanium discs showed unique surface characteristics (Fig. 2). The machined-surface presented the lowest value of average roughness (Ra=0.537±0.051 µm), root mean square roughness (Rq=0.681±0.059 µm) and valley depth (Rz=2.169±0.239 µm), while the SLA-treated group showed increased values (Ra=1.285±0.025 µm, Rq=1.759±0.037 µm, Rz=6.652±0.797 µm). The SLA-treated and microgroove-formed discs showed the highest values in the average roughness, root mean square roughness, and valley depth, which were 22.351±2.766 µm, 25.202±2.472 µm, and 46.161±4.904 µm, respectively (Table 1).


Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study.

Lee HJ, Lee J, Lee JT, Hong JS, Lim BS, Park HJ, Kim YK, Kim TI - J Periodontal Implant Sci (2015)

Laser scanning confocal microscopic images of titanium discs. A, machined-surface discs; B, sandblasted, large-grit, acid-etched (SLA)-treated discs; C, SLA-treated and microgroove-formed discs. Bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4485062&req=5

Figure 2: Laser scanning confocal microscopic images of titanium discs. A, machined-surface discs; B, sandblasted, large-grit, acid-etched (SLA)-treated discs; C, SLA-treated and microgroove-formed discs. Bar=100 µm.
Mentions: Each group of titanium discs showed unique surface characteristics (Fig. 2). The machined-surface presented the lowest value of average roughness (Ra=0.537±0.051 µm), root mean square roughness (Rq=0.681±0.059 µm) and valley depth (Rz=2.169±0.239 µm), while the SLA-treated group showed increased values (Ra=1.285±0.025 µm, Rq=1.759±0.037 µm, Rz=6.652±0.797 µm). The SLA-treated and microgroove-formed discs showed the highest values in the average roughness, root mean square roughness, and valley depth, which were 22.351±2.766 µm, 25.202±2.472 µm, and 46.161±4.904 µm, respectively (Table 1).

Bottom Line: Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs.After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens.Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 µm).

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.

ABSTRACT

Purpose: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and tissues.

Methods: Three types of commercially pure titanium discs were prepared; machined-surface discs (A), sandblasted, large-grit, acid-etched (SLA)-treated discs (B), SLA and microgroove-formed discs (C). After surface topography of the discs was examined by confocal laser scanning electron microscopy, water contact angle and surface energy were measured. Human gingival fibroblasts (hGFs) and murine osteoblastic cells (MC3T3-E1) were seeded onto the titanium discs for immunofluorescence assay of adhesion proteins. Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs. After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens.

Results: Group C presented the lowest water contact angle (62.89±5.66 θ), highest surface energy (45±1.2 mN/m), and highest surface roughness (Ra=22.351±2.766 µm). The expression of adhesion molecules of hGFs and MC3T30E1 cells was prominent in group C. Titanium implants with microgrooves on the coronal portion showed firm adhesion to peri-implant soft tissue.

Conclusions: Microgrooves on the titanium surface promoted the adhesion of gingival fibroblasts and osteoblastic cells, as well as favorable peri-implant soft tissue sealing.

No MeSH data available.


Related in: MedlinePlus