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Chemical and Metabolic Profiling of Si-Ni Decoction Analogous Formulae by High performance Liquid Chromatography-Mass Spectrometry.

Chen Q, Xiao S, Li Z, Ai N, Fan X - Sci Rep (2015)

Bottom Line: To improve our understanding on their therapeutic effects, it's critical to unveil chemical compositions of TCM formula, the predominant form of therapy in TCM.Analysis on SNs-treated rats detected 55 prototype compounds and 39 metabolites in the systemic circulation in vivo, which may contribute directly to their observed clinical efficacies.This approach offers great advantage to speed up identification of chemical compositions of formula and reveal the difference among these analogous formulae that may be related to diverse clinical effects.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
Along with an indispensable role in healthcare system of China for centuries, Traditional Chinese Medicine (TCM) shows increasing usages as complementary therapy in western countries. To improve our understanding on their therapeutic effects, it's critical to unveil chemical compositions of TCM formula, the predominant form of therapy in TCM. However, intrinsic chemical complexity makes it a challenging task to perform analysis on each individual TCM formula even with most current state-of-art analytic techniques available. In this work we approached this question by focusing on analogous formulae, a unique category of TCM formulae grouped together based on shared herbs and/or similar TCM syndromes. Systematic chemical profiling on five Si-Ni decoctions (SNs) for cardiovascular diseases was performed by multistage MS and high-resolution MS (HR-MS) experiments. A total of 83 compounds, including alkaloids, flavonoids, ginsenosides, bile acids and triterpenoids, were described. Analysis on SNs-treated rats detected 55 prototype compounds and 39 metabolites in the systemic circulation in vivo, which may contribute directly to their observed clinical efficacies. This approach offers great advantage to speed up identification of chemical compositions of formula and reveal the difference among these analogous formulae that may be related to diverse clinical effects.

No MeSH data available.


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Comparative analysis scheme of Si-Ni decoction analogous formulae in vitro and in vivo.
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f1: Comparative analysis scheme of Si-Ni decoction analogous formulae in vitro and in vivo.

Mentions: Si-Ni decoction, described in ancient Zhang Zhongjing’s herbal formulae (Shanghan Lun, which is a Chinese medical treatise compiled by Zhang Zhongjing to treat epidemic infectious diseases causing fevers), is a typical TCM formula which has been used for over 2000 years. It consists of Aconiti lateralis radix (Fuzi, ranunculaceae), Glycyrrhizae radix et rhizome praeparata cum melle (Zhigancao, Fabaceae) and Zingiberis rhizoma (Ganjiang, Zingibeaceae). SNs includes Si-Ni decoction (SIN), Fuling Si-Ni decoction (FSIN), Ginseng Si-Ni decoction (RSIN), Tongmai Si-Ni decoction (TSIN) and Tongmai Si-Ni with Suis fellis pulvis decoction (ZSIN) with different content ratio or adding Suis fellis pulvis (Zhudanfen, Sus scrofa domestica Brisson) or Ginseng radix et rhizoma (Renshen, Araliaceae) and/or Poria (Fuling, Polypores). SNs have been used to prevent or treat cardiovascular disease for many years12131415, but their components are still poorly understood16. In this paper, an integrated approach by combining pre-classification strategy17 and diagnostic fragment-ion-based extension strategy1819 was developed to characterize and compare the chemical components of SNs systematically in vitro and in vivo based on multistage MS and HR-MS (Fig. 1). The pre-classification strategy17 on multiple spectrums of TCM formula was previous proposed by our group on the basis that each class of nature products, such as flavonoids, alkaloids, and saponins, shares a characteristic carbon skeleton or the same structural units. It not only improved the accuracy but also sped up the identification of compounds from chemical databases and relevant literatures. Until now chemical differentiation study has not yet performed on these five formulae and very little is known about the chemical profiling of SNs, especially those in vivo. Results from this work will facilitate further research in pharmacological basis for clinical application of these five formulae.


Chemical and Metabolic Profiling of Si-Ni Decoction Analogous Formulae by High performance Liquid Chromatography-Mass Spectrometry.

Chen Q, Xiao S, Li Z, Ai N, Fan X - Sci Rep (2015)

Comparative analysis scheme of Si-Ni decoction analogous formulae in vitro and in vivo.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4484491&req=5

f1: Comparative analysis scheme of Si-Ni decoction analogous formulae in vitro and in vivo.
Mentions: Si-Ni decoction, described in ancient Zhang Zhongjing’s herbal formulae (Shanghan Lun, which is a Chinese medical treatise compiled by Zhang Zhongjing to treat epidemic infectious diseases causing fevers), is a typical TCM formula which has been used for over 2000 years. It consists of Aconiti lateralis radix (Fuzi, ranunculaceae), Glycyrrhizae radix et rhizome praeparata cum melle (Zhigancao, Fabaceae) and Zingiberis rhizoma (Ganjiang, Zingibeaceae). SNs includes Si-Ni decoction (SIN), Fuling Si-Ni decoction (FSIN), Ginseng Si-Ni decoction (RSIN), Tongmai Si-Ni decoction (TSIN) and Tongmai Si-Ni with Suis fellis pulvis decoction (ZSIN) with different content ratio or adding Suis fellis pulvis (Zhudanfen, Sus scrofa domestica Brisson) or Ginseng radix et rhizoma (Renshen, Araliaceae) and/or Poria (Fuling, Polypores). SNs have been used to prevent or treat cardiovascular disease for many years12131415, but their components are still poorly understood16. In this paper, an integrated approach by combining pre-classification strategy17 and diagnostic fragment-ion-based extension strategy1819 was developed to characterize and compare the chemical components of SNs systematically in vitro and in vivo based on multistage MS and HR-MS (Fig. 1). The pre-classification strategy17 on multiple spectrums of TCM formula was previous proposed by our group on the basis that each class of nature products, such as flavonoids, alkaloids, and saponins, shares a characteristic carbon skeleton or the same structural units. It not only improved the accuracy but also sped up the identification of compounds from chemical databases and relevant literatures. Until now chemical differentiation study has not yet performed on these five formulae and very little is known about the chemical profiling of SNs, especially those in vivo. Results from this work will facilitate further research in pharmacological basis for clinical application of these five formulae.

Bottom Line: To improve our understanding on their therapeutic effects, it's critical to unveil chemical compositions of TCM formula, the predominant form of therapy in TCM.Analysis on SNs-treated rats detected 55 prototype compounds and 39 metabolites in the systemic circulation in vivo, which may contribute directly to their observed clinical efficacies.This approach offers great advantage to speed up identification of chemical compositions of formula and reveal the difference among these analogous formulae that may be related to diverse clinical effects.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
Along with an indispensable role in healthcare system of China for centuries, Traditional Chinese Medicine (TCM) shows increasing usages as complementary therapy in western countries. To improve our understanding on their therapeutic effects, it's critical to unveil chemical compositions of TCM formula, the predominant form of therapy in TCM. However, intrinsic chemical complexity makes it a challenging task to perform analysis on each individual TCM formula even with most current state-of-art analytic techniques available. In this work we approached this question by focusing on analogous formulae, a unique category of TCM formulae grouped together based on shared herbs and/or similar TCM syndromes. Systematic chemical profiling on five Si-Ni decoctions (SNs) for cardiovascular diseases was performed by multistage MS and high-resolution MS (HR-MS) experiments. A total of 83 compounds, including alkaloids, flavonoids, ginsenosides, bile acids and triterpenoids, were described. Analysis on SNs-treated rats detected 55 prototype compounds and 39 metabolites in the systemic circulation in vivo, which may contribute directly to their observed clinical efficacies. This approach offers great advantage to speed up identification of chemical compositions of formula and reveal the difference among these analogous formulae that may be related to diverse clinical effects.

No MeSH data available.


Related in: MedlinePlus