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Dietary protein and lifespan across the metamorphic boundary: protein-restricted larvae develop into short-lived adults.

Runagall-McNaull A, Bonduriansky R, Crean AJ - Sci Rep (2015)

Bottom Line: We found that adult male and female lifespan was shortest when larvae were fed a protein restricted diet.Adult lifespan was unaffected by larval dietary carbohydrate.We propose that larval and adult protein sources are used for distinct metabolic tasks: during development, dietary protein is used to build a durable soma that enhances adult lifespan, although excessive protein consumption partially reverses this effect.

View Article: PubMed Central - PubMed

Affiliation: Evolution &Ecology Research Centre, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, 2052 Australia.

ABSTRACT
Restriction of nutrients in the adult diet extends lifespan across a diverse range of species, but less is known about the long-term effects of developmental dietary restriction. In particular, it is not known whether adult lifespan is influenced by developmental caloric restriction or macronutrient balance. We used the nutritional geometry approach to independently manipulate protein and carbohydrate contents of the larval diet in the neriid fly, Telostylinus angusticollis, and measured adult lifespan. We found that adult male and female lifespan was shortest when larvae were fed a protein restricted diet. Thus, protein restriction in the larval diet has the opposite effect of protein restriction in the adult diet (which prolongs life in this species and across a wide range of taxa). Adult lifespan was unaffected by larval dietary carbohydrate. These patterns persisted after controlling for larval diet effects on adult body size. We propose that larval and adult protein sources are used for distinct metabolic tasks: during development, dietary protein is used to build a durable soma that enhances adult lifespan, although excessive protein consumption partially reverses this effect.

No MeSH data available.


Nutrient space with six rails representing each P:C ratio used in this experiment.Points on each rail represent treatment diets with varying quantities of protein and carbohydrate (g) per 1 L of dry cocopeat and 600 mL water.
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f1: Nutrient space with six rails representing each P:C ratio used in this experiment.Points on each rail represent treatment diets with varying quantities of protein and carbohydrate (g) per 1 L of dry cocopeat and 600 mL water.

Mentions: We used a nutritional geometry approach21 to examine the separate and interactive effects of protein and carbohydrate in the larval diet on adult lifespan of the neriid fly Telostylinus angusticollis (Fig. 1). Previous studies in this species have shown that restriction of adult dietary protein increases lifespan by 67%19. In addition, previous studies have shown that larvae reared on a nutrient-poor medium develop into smaller adults16 that are more susceptible to starvation19. Increased concentrations of carbohydrates in the larval diet increases egg-to-adult viability and adult body size, whereas protein in the larval diet enhances the expression of male secondary sexual traits but decreases larval survival22. The effects of protein and carbohydrate consumption by larvae on adult lifespan have not been examined previously. Adults were provided with both protein and sugar to test for effects of developmental dietary restriction independently of adult dietary restriction, and focal individuals were housed with a standardised individual of the opposite sex to allow for reproduction.


Dietary protein and lifespan across the metamorphic boundary: protein-restricted larvae develop into short-lived adults.

Runagall-McNaull A, Bonduriansky R, Crean AJ - Sci Rep (2015)

Nutrient space with six rails representing each P:C ratio used in this experiment.Points on each rail represent treatment diets with varying quantities of protein and carbohydrate (g) per 1 L of dry cocopeat and 600 mL water.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4484247&req=5

f1: Nutrient space with six rails representing each P:C ratio used in this experiment.Points on each rail represent treatment diets with varying quantities of protein and carbohydrate (g) per 1 L of dry cocopeat and 600 mL water.
Mentions: We used a nutritional geometry approach21 to examine the separate and interactive effects of protein and carbohydrate in the larval diet on adult lifespan of the neriid fly Telostylinus angusticollis (Fig. 1). Previous studies in this species have shown that restriction of adult dietary protein increases lifespan by 67%19. In addition, previous studies have shown that larvae reared on a nutrient-poor medium develop into smaller adults16 that are more susceptible to starvation19. Increased concentrations of carbohydrates in the larval diet increases egg-to-adult viability and adult body size, whereas protein in the larval diet enhances the expression of male secondary sexual traits but decreases larval survival22. The effects of protein and carbohydrate consumption by larvae on adult lifespan have not been examined previously. Adults were provided with both protein and sugar to test for effects of developmental dietary restriction independently of adult dietary restriction, and focal individuals were housed with a standardised individual of the opposite sex to allow for reproduction.

Bottom Line: We found that adult male and female lifespan was shortest when larvae were fed a protein restricted diet.Adult lifespan was unaffected by larval dietary carbohydrate.We propose that larval and adult protein sources are used for distinct metabolic tasks: during development, dietary protein is used to build a durable soma that enhances adult lifespan, although excessive protein consumption partially reverses this effect.

View Article: PubMed Central - PubMed

Affiliation: Evolution &Ecology Research Centre, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, 2052 Australia.

ABSTRACT
Restriction of nutrients in the adult diet extends lifespan across a diverse range of species, but less is known about the long-term effects of developmental dietary restriction. In particular, it is not known whether adult lifespan is influenced by developmental caloric restriction or macronutrient balance. We used the nutritional geometry approach to independently manipulate protein and carbohydrate contents of the larval diet in the neriid fly, Telostylinus angusticollis, and measured adult lifespan. We found that adult male and female lifespan was shortest when larvae were fed a protein restricted diet. Thus, protein restriction in the larval diet has the opposite effect of protein restriction in the adult diet (which prolongs life in this species and across a wide range of taxa). Adult lifespan was unaffected by larval dietary carbohydrate. These patterns persisted after controlling for larval diet effects on adult body size. We propose that larval and adult protein sources are used for distinct metabolic tasks: during development, dietary protein is used to build a durable soma that enhances adult lifespan, although excessive protein consumption partially reverses this effect.

No MeSH data available.