Limits...
Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice.

Zhang S, Kuang Z, Zhang X - Int J Environ Res Public Health (2015)

Bottom Line: After 55, 59, and 75 h of exposure, the experimental groups were successively removed from the chamber to monitor physiological indicators.NE and ANGII are vasoconstrictors involved in systemic vasoconstriction especially in the superficial areas of the body and conducive to increased blood pressure.The increase in the blood lipid levels of TG, LDL-C, TC, and LDL-C/HDL-C further aggravated CVD.

View Article: PubMed Central - PubMed

Affiliation: Hebei Provincial Meteorological Bureau, 178 Tiyu South Street, Shijiazhuang 050021, China. zhangsy@cma.gov.cn.

ABSTRACT
This study analyzes the mechanism of influence of heat waves with strong sudden cooling on cardiovascular diseases (CVD) in ApoE-/- mice. The process of heat waves with strong sudden cooling was simulated with a TEM1880 meteorological-environment simulation chamber according to the data obtained at 5 a.m. of 19 June 2006 to 11 p.m. of 22 June 2006. Forty-eight ApoE-/- mice were divided into six blocks based on their weight. Two mice from each block were randomly assigned to control, heat wave, temperature drop, and rewarming temperature groups. The experimental groups were transferred into the climate simulator chamber for exposure to the simulated heat wave process with strong sudden temperature drop. After 55, 59, and 75 h of exposure, the experimental groups were successively removed from the chamber to monitor physiological indicators. Blood samples were collected by decollation, and the hearts were harvested in all groups. The levels of heat stress factors (HSP60, SOD, TNF, sICAM-1, HIF-1α), cold stress factors (NE, EPI), vasoconstrictor factors (ANGII, ET-1, NO), and four items of blood lipid (TC, TG, HDL-C, and LDL-C) were measured in each ApoE-/- mouse. Results showed that the heat waves increased the levels of heat stress factors except SOD decreased, and decreased the levels of vasoconstrictor factors and blood lipid factors except TC increased. The strong sudden temperature drop in the heat wave process increased the levels of cold stress factors, vasoconstrictor factors and four blood lipid items (except the level of HDL-C which decreased) and decreased the levels of heat stress factors (except the level of SOD which increased). The analysis showed that heat waves could enhance atherosclerosis of ApoE-/- mice. The strong sudden temperature drop during the heat wave process increased the plasma concentrations of NE and ANGII, which indicates SNS activation, and resulted in increased blood pressure. NE and ANGII are vasoconstrictors involved in systemic vasoconstriction especially in the superficial areas of the body and conducive to increased blood pressure. The increase in the blood lipid levels of TG, LDL-C, TC, and LDL-C/HDL-C further aggravated CVD. This paper explored the influence mechanism of the heat waves with sudden cooling on CVD in ApoE-/- mice.

No MeSH data available.


Related in: MedlinePlus

Experiment temperature curve (arrows represent sampling time points).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4483669&req=5

ijerph-12-05743-f001: Experiment temperature curve (arrows represent sampling time points).

Mentions: The simulation curve is shown in Figure 1. The experimental temperature of the control group was set at 27 °C. The experimental groups were heat wave, temperature drop, and rewarming temperature groups, which correspond to the three sample time points. The heat wave, temperature drop, and rewarming temperature groups were used to assess the effects of heat stimulation, strong sudden temperature drop, and rewarming temperature on experimental mice, respectively.


Effects of Simulated Heat Waves with Strong Sudden Cooling Weather on ApoE Knockout Mice.

Zhang S, Kuang Z, Zhang X - Int J Environ Res Public Health (2015)

Experiment temperature curve (arrows represent sampling time points).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483669&req=5

ijerph-12-05743-f001: Experiment temperature curve (arrows represent sampling time points).
Mentions: The simulation curve is shown in Figure 1. The experimental temperature of the control group was set at 27 °C. The experimental groups were heat wave, temperature drop, and rewarming temperature groups, which correspond to the three sample time points. The heat wave, temperature drop, and rewarming temperature groups were used to assess the effects of heat stimulation, strong sudden temperature drop, and rewarming temperature on experimental mice, respectively.

Bottom Line: After 55, 59, and 75 h of exposure, the experimental groups were successively removed from the chamber to monitor physiological indicators.NE and ANGII are vasoconstrictors involved in systemic vasoconstriction especially in the superficial areas of the body and conducive to increased blood pressure.The increase in the blood lipid levels of TG, LDL-C, TC, and LDL-C/HDL-C further aggravated CVD.

View Article: PubMed Central - PubMed

Affiliation: Hebei Provincial Meteorological Bureau, 178 Tiyu South Street, Shijiazhuang 050021, China. zhangsy@cma.gov.cn.

ABSTRACT
This study analyzes the mechanism of influence of heat waves with strong sudden cooling on cardiovascular diseases (CVD) in ApoE-/- mice. The process of heat waves with strong sudden cooling was simulated with a TEM1880 meteorological-environment simulation chamber according to the data obtained at 5 a.m. of 19 June 2006 to 11 p.m. of 22 June 2006. Forty-eight ApoE-/- mice were divided into six blocks based on their weight. Two mice from each block were randomly assigned to control, heat wave, temperature drop, and rewarming temperature groups. The experimental groups were transferred into the climate simulator chamber for exposure to the simulated heat wave process with strong sudden temperature drop. After 55, 59, and 75 h of exposure, the experimental groups were successively removed from the chamber to monitor physiological indicators. Blood samples were collected by decollation, and the hearts were harvested in all groups. The levels of heat stress factors (HSP60, SOD, TNF, sICAM-1, HIF-1α), cold stress factors (NE, EPI), vasoconstrictor factors (ANGII, ET-1, NO), and four items of blood lipid (TC, TG, HDL-C, and LDL-C) were measured in each ApoE-/- mouse. Results showed that the heat waves increased the levels of heat stress factors except SOD decreased, and decreased the levels of vasoconstrictor factors and blood lipid factors except TC increased. The strong sudden temperature drop in the heat wave process increased the levels of cold stress factors, vasoconstrictor factors and four blood lipid items (except the level of HDL-C which decreased) and decreased the levels of heat stress factors (except the level of SOD which increased). The analysis showed that heat waves could enhance atherosclerosis of ApoE-/- mice. The strong sudden temperature drop during the heat wave process increased the plasma concentrations of NE and ANGII, which indicates SNS activation, and resulted in increased blood pressure. NE and ANGII are vasoconstrictors involved in systemic vasoconstriction especially in the superficial areas of the body and conducive to increased blood pressure. The increase in the blood lipid levels of TG, LDL-C, TC, and LDL-C/HDL-C further aggravated CVD. This paper explored the influence mechanism of the heat waves with sudden cooling on CVD in ApoE-/- mice.

No MeSH data available.


Related in: MedlinePlus