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Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains.

Merino S, de Mendoza E, Canals R, Tomás JM - Mar Drugs (2015)

Bottom Line: Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains).Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer.A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, Spain. smerino@ub.edu.

ABSTRACT
The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens.

No MeSH data available.


Related in: MedlinePlus

The A. salmonicida subsp. pectinolytica strain 34melT putative genes for the LPS O-antigen cluster in orange. The genes, numbered according to the ORF number, were named according to their similarity, as found by their encoding proteins, with proteins of well characterized functions.
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marinedrugs-13-03791-f005: The A. salmonicida subsp. pectinolytica strain 34melT putative genes for the LPS O-antigen cluster in orange. The genes, numbered according to the ORF number, were named according to their similarity, as found by their encoding proteins, with proteins of well characterized functions.

Mentions: When we inspected all the currently available Aeromonas salmonicida genomes, we found two for A. salmonicida subsp. salmonicida strains A449 and 01-B526, one for A. salmonicida subsp. achromogenes strain AS03, one for A. salmonicida subsp. masoucida strain NBRC 13784, one for A. salmonicida subsp. pectinolytica strain 34melT, and none for A. salmonicida subsp. smithia. Despite the different genome annotations (in part because these DNA regions had not been properly studied) with a more accurate comparative genomic analysis, we can conclude that in all the A. salmonicida genomes, besides the one for A. salmonicida subsp. pectinolytica strain 34melT, the wbsalmo is nearly identical. Furthermore, the chromosomal location between the oprM and UDP-ep genes is conserved among them. However, in the A. salmonicida subsp. pectinolytica strain 34melT genome, we found a putative wb cluster completely different. Briefly, the rml genes for rhamnose biosynthesis are not together, wzm-wzt are not present, and besides finding an oprM gene upstream, downstream of the rmlC seems to be a region with partial genes and encoded bacteriophage proteins (see Figure 5 and Table 3 ).


Functional Genomics of the Aeromonas salmonicida Lipopolysaccharide O-Antigen and A-Layer from Typical and Atypical Strains.

Merino S, de Mendoza E, Canals R, Tomás JM - Mar Drugs (2015)

The A. salmonicida subsp. pectinolytica strain 34melT putative genes for the LPS O-antigen cluster in orange. The genes, numbered according to the ORF number, were named according to their similarity, as found by their encoding proteins, with proteins of well characterized functions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483657&req=5

marinedrugs-13-03791-f005: The A. salmonicida subsp. pectinolytica strain 34melT putative genes for the LPS O-antigen cluster in orange. The genes, numbered according to the ORF number, were named according to their similarity, as found by their encoding proteins, with proteins of well characterized functions.
Mentions: When we inspected all the currently available Aeromonas salmonicida genomes, we found two for A. salmonicida subsp. salmonicida strains A449 and 01-B526, one for A. salmonicida subsp. achromogenes strain AS03, one for A. salmonicida subsp. masoucida strain NBRC 13784, one for A. salmonicida subsp. pectinolytica strain 34melT, and none for A. salmonicida subsp. smithia. Despite the different genome annotations (in part because these DNA regions had not been properly studied) with a more accurate comparative genomic analysis, we can conclude that in all the A. salmonicida genomes, besides the one for A. salmonicida subsp. pectinolytica strain 34melT, the wbsalmo is nearly identical. Furthermore, the chromosomal location between the oprM and UDP-ep genes is conserved among them. However, in the A. salmonicida subsp. pectinolytica strain 34melT genome, we found a putative wb cluster completely different. Briefly, the rml genes for rhamnose biosynthesis are not together, wzm-wzt are not present, and besides finding an oprM gene upstream, downstream of the rmlC seems to be a region with partial genes and encoded bacteriophage proteins (see Figure 5 and Table 3 ).

Bottom Line: Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains).Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer.A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona 08071, Spain. smerino@ub.edu.

ABSTRACT
The A. salmonicida A450 LPS O-antigen, encoded by the wbsalmo gene cluster, is exported through an ABC-2 transporter-dependent pathway. It represents the first example of an O-antigen LPS polysaccharide with three different monosaccharides in their repeating unit assembled by this pathway. Until now, only repeating units with one or two different monosaccharides have been described. Functional genomic analysis of this wbsalmo region is mostly in agreement with the LPS O-antigen structure of acetylated l-rhamnose (Rha), d-glucose (Glc), and 2-amino-2-deoxy-d-mannose (ManN). Between genes of the wbsalmo we found the genes responsible for the biosynthesis and assembly of the S-layer (named A-layer in these strains). Through comparative genomic analysis and in-frame deletions of some of the genes, we concluded that all the A. salmonicida typical and atypical strains, other than A. salmonicida subsp. pectinolytica strains, shared the same wbsalmo and presence of A-layer. A. salmonicida subsp. pectinolytica strains lack wbsalmo and A-layer, two major virulence factors, and this could be the reason they are the only ones not found as fish pathogens.

No MeSH data available.


Related in: MedlinePlus