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Use of Natural Antimicrobial Peptides and Bacterial Biopolymers for Cultured Pearl Production.

Simon-Colin C, Gueguen Y, Bachere E, Kouzayha A, Saulnier D, Gayet N, Guezennec J - Mar Drugs (2015)

Bottom Line: In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls.The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination.These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures.

View Article: PubMed Central - PubMed

Affiliation: Ifremer, Centre de Brest, BP 70, 29280 Plouzané, France. Christelle.Simon.Colin@ifremer.fr.

ABSTRACT
Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with two marine bacterial exopolymers as alternative treatment agents. In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls. The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination. The survival data were similar to that reported for antibiotic treatments. These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures.

No MeSH data available.


Related in: MedlinePlus

Pearl oysters culture after grating. (A) During the first 40 days after the graft, pearl oysters are cultured in retention baskets to assess the rejection of nucleus; (B) The shells are then drilled on the side and pearl oysters are tied with a nylon thread to a cord.
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marinedrugs-13-03732-f004: Pearl oysters culture after grating. (A) During the first 40 days after the graft, pearl oysters are cultured in retention baskets to assess the rejection of nucleus; (B) The shells are then drilled on the side and pearl oysters are tied with a nylon thread to a cord.

Mentions: Approximatively, 500 grafts were performed for each nuclei condition. Nucleus retention and oyster mortality rates were evaluated after 40 days and 15 months of immersion under natural conditions (Figure 4). After 40 days no significant differences were observed within the four different experimental conditions (A, B, C and D). However, the EPS Mo245 associated with the tachyplesin (B) showed a better retention rate than the non coated nucleus (F) and as good as the commercial one (E) (Figure 5A,B). Additionally, coating with tachyplesin-EPSs solution induced a lesser oyster mortality compared to non-treated samples and a similar mortality to commercialized antibiotic treated nuclei.


Use of Natural Antimicrobial Peptides and Bacterial Biopolymers for Cultured Pearl Production.

Simon-Colin C, Gueguen Y, Bachere E, Kouzayha A, Saulnier D, Gayet N, Guezennec J - Mar Drugs (2015)

Pearl oysters culture after grating. (A) During the first 40 days after the graft, pearl oysters are cultured in retention baskets to assess the rejection of nucleus; (B) The shells are then drilled on the side and pearl oysters are tied with a nylon thread to a cord.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483653&req=5

marinedrugs-13-03732-f004: Pearl oysters culture after grating. (A) During the first 40 days after the graft, pearl oysters are cultured in retention baskets to assess the rejection of nucleus; (B) The shells are then drilled on the side and pearl oysters are tied with a nylon thread to a cord.
Mentions: Approximatively, 500 grafts were performed for each nuclei condition. Nucleus retention and oyster mortality rates were evaluated after 40 days and 15 months of immersion under natural conditions (Figure 4). After 40 days no significant differences were observed within the four different experimental conditions (A, B, C and D). However, the EPS Mo245 associated with the tachyplesin (B) showed a better retention rate than the non coated nucleus (F) and as good as the commercial one (E) (Figure 5A,B). Additionally, coating with tachyplesin-EPSs solution induced a lesser oyster mortality compared to non-treated samples and a similar mortality to commercialized antibiotic treated nuclei.

Bottom Line: In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls.The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination.These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures.

View Article: PubMed Central - PubMed

Affiliation: Ifremer, Centre de Brest, BP 70, 29280 Plouzané, France. Christelle.Simon.Colin@ifremer.fr.

ABSTRACT
Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with two marine bacterial exopolymers as alternative treatment agents. In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls. The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination. The survival data were similar to that reported for antibiotic treatments. These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures.

No MeSH data available.


Related in: MedlinePlus