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Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera caespitosa Miki.

Lv Y, Shan X, Zhao X, Cai C, Zhao X, Lang Y, Zhu H, Yu G - Mar Drugs (2015)

Bottom Line: The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth.ZCMP also promoted macrophage phagocytosis.These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. lvyoujing1988@163.com.

ABSTRACT
An apigalacturonan (AGA)-rich polysaccharide, ZCMP, was isolated from the sea grass Zostera caespitosa Miki. The depolymerized fragments derived from ZCMP were obtained by either acidic degradation or pectinase degradation, and their structures were characterized by electrospray ionization collision-induced-dissociation mass spectrometry (ESI-CID-MS2) and nuclear magnetic resonance (NMR) spectroscopy. The average molecular weight of ZCMP was 77.2 kD and it consisted of galacturonic acid (GalA), apiosefuranose (Api), galactose (Gal), rhamnose (Rha), arabinose (Ara), xylose (Xyl), and mannose (Man), at a molar ratio of 51.4꞉15.5꞉6.0꞉11.8꞉4.2꞉4.4꞉4.2. There were two regions of AGA (70%) and rhamnogalacturonan-I (RG-Ι, 30%) in ZCMP. AGA was composed of an α-1,4-D-galactopyranosyluronan backbone mainly substituted at the O-3 position by single Api residues. RG-Ι possessed a backbone of repeating disaccharide units of →4GalAα1,2Rhaα1→, with a few α-L-arabinose and β-D-galactose residues as side chains. The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth. ZCMP also promoted macrophage phagocytosis. These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

No MeSH data available.


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Proposed structural model of ZCMP.
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marinedrugs-13-03710-f010: Proposed structural model of ZCMP.

Mentions: According to the proposed general structural model for lemnan [7], zosterin [10], pectin [32], and the results obtained in the present study, we propose the following structural model for Z. caespitosa Miki polysaccharide ZCMP (Figure 10). ZCMP is composed of AGA and RG-Ι regions. AGA has a backbone of α-1,4-d-galactopyranosyluronan with an extremely low degree of etherification, whereas the side chains were linked to the O-3 of GalA by most of the single Api residues and minor short (1→3′)-linked β-d-Api oligosaccharides with different degree of polymerization (<5). RG-Ι has a backbone of repeating 4-linked GalA and 2-linked Rha with minor 5-linked α-l-Ara residues and 4-linked β-d-Gal residues as side chains.


Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera caespitosa Miki.

Lv Y, Shan X, Zhao X, Cai C, Zhao X, Lang Y, Zhu H, Yu G - Mar Drugs (2015)

Proposed structural model of ZCMP.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483652&req=5

marinedrugs-13-03710-f010: Proposed structural model of ZCMP.
Mentions: According to the proposed general structural model for lemnan [7], zosterin [10], pectin [32], and the results obtained in the present study, we propose the following structural model for Z. caespitosa Miki polysaccharide ZCMP (Figure 10). ZCMP is composed of AGA and RG-Ι regions. AGA has a backbone of α-1,4-d-galactopyranosyluronan with an extremely low degree of etherification, whereas the side chains were linked to the O-3 of GalA by most of the single Api residues and minor short (1→3′)-linked β-d-Api oligosaccharides with different degree of polymerization (<5). RG-Ι has a backbone of repeating 4-linked GalA and 2-linked Rha with minor 5-linked α-l-Ara residues and 4-linked β-d-Gal residues as side chains.

Bottom Line: The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth.ZCMP also promoted macrophage phagocytosis.These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. lvyoujing1988@163.com.

ABSTRACT
An apigalacturonan (AGA)-rich polysaccharide, ZCMP, was isolated from the sea grass Zostera caespitosa Miki. The depolymerized fragments derived from ZCMP were obtained by either acidic degradation or pectinase degradation, and their structures were characterized by electrospray ionization collision-induced-dissociation mass spectrometry (ESI-CID-MS2) and nuclear magnetic resonance (NMR) spectroscopy. The average molecular weight of ZCMP was 77.2 kD and it consisted of galacturonic acid (GalA), apiosefuranose (Api), galactose (Gal), rhamnose (Rha), arabinose (Ara), xylose (Xyl), and mannose (Man), at a molar ratio of 51.4꞉15.5꞉6.0꞉11.8꞉4.2꞉4.4꞉4.2. There were two regions of AGA (70%) and rhamnogalacturonan-I (RG-Ι, 30%) in ZCMP. AGA was composed of an α-1,4-D-galactopyranosyluronan backbone mainly substituted at the O-3 position by single Api residues. RG-Ι possessed a backbone of repeating disaccharide units of →4GalAα1,2Rhaα1→, with a few α-L-arabinose and β-D-galactose residues as side chains. The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth. ZCMP also promoted macrophage phagocytosis. These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

No MeSH data available.


Related in: MedlinePlus