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Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera caespitosa Miki.

Lv Y, Shan X, Zhao X, Cai C, Zhao X, Lang Y, Zhu H, Yu G - Mar Drugs (2015)

Bottom Line: The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth.ZCMP also promoted macrophage phagocytosis.These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. lvyoujing1988@163.com.

ABSTRACT
An apigalacturonan (AGA)-rich polysaccharide, ZCMP, was isolated from the sea grass Zostera caespitosa Miki. The depolymerized fragments derived from ZCMP were obtained by either acidic degradation or pectinase degradation, and their structures were characterized by electrospray ionization collision-induced-dissociation mass spectrometry (ESI-CID-MS2) and nuclear magnetic resonance (NMR) spectroscopy. The average molecular weight of ZCMP was 77.2 kD and it consisted of galacturonic acid (GalA), apiosefuranose (Api), galactose (Gal), rhamnose (Rha), arabinose (Ara), xylose (Xyl), and mannose (Man), at a molar ratio of 51.4꞉15.5꞉6.0꞉11.8꞉4.2꞉4.4꞉4.2. There were two regions of AGA (70%) and rhamnogalacturonan-I (RG-Ι, 30%) in ZCMP. AGA was composed of an α-1,4-D-galactopyranosyluronan backbone mainly substituted at the O-3 position by single Api residues. RG-Ι possessed a backbone of repeating disaccharide units of →4GalAα1,2Rhaα1→, with a few α-L-arabinose and β-D-galactose residues as side chains. The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth. ZCMP also promoted macrophage phagocytosis. These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

No MeSH data available.


Related in: MedlinePlus

Flow chart of the degradation process of ZCMP.
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marinedrugs-13-03710-f002: Flow chart of the degradation process of ZCMP.

Mentions: ZCMP was shown to be sensitive under acidic conditions such as 0.1 mol/L HCl and H2SO4, and the Api residues were rapidly released as monosaccharides in our model experiment. A three-level acid solution (0.1 mol/L CH3COOH, 0.2 mol/L HCl, and 0.5 mol/L HCl) was added to degrade the polysaccharide progressively, and pectinase was also used to generate oligosaccharides with methyl esters and acetyl groups. The depolymerized oligosaccharides were collected by precipitation using different concentrations of ethanol. The degradation process of ZCMP is shown in Figure 2.


Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera caespitosa Miki.

Lv Y, Shan X, Zhao X, Cai C, Zhao X, Lang Y, Zhu H, Yu G - Mar Drugs (2015)

Flow chart of the degradation process of ZCMP.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483652&req=5

marinedrugs-13-03710-f002: Flow chart of the degradation process of ZCMP.
Mentions: ZCMP was shown to be sensitive under acidic conditions such as 0.1 mol/L HCl and H2SO4, and the Api residues were rapidly released as monosaccharides in our model experiment. A three-level acid solution (0.1 mol/L CH3COOH, 0.2 mol/L HCl, and 0.5 mol/L HCl) was added to degrade the polysaccharide progressively, and pectinase was also used to generate oligosaccharides with methyl esters and acetyl groups. The depolymerized oligosaccharides were collected by precipitation using different concentrations of ethanol. The degradation process of ZCMP is shown in Figure 2.

Bottom Line: The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth.ZCMP also promoted macrophage phagocytosis.These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. lvyoujing1988@163.com.

ABSTRACT
An apigalacturonan (AGA)-rich polysaccharide, ZCMP, was isolated from the sea grass Zostera caespitosa Miki. The depolymerized fragments derived from ZCMP were obtained by either acidic degradation or pectinase degradation, and their structures were characterized by electrospray ionization collision-induced-dissociation mass spectrometry (ESI-CID-MS2) and nuclear magnetic resonance (NMR) spectroscopy. The average molecular weight of ZCMP was 77.2 kD and it consisted of galacturonic acid (GalA), apiosefuranose (Api), galactose (Gal), rhamnose (Rha), arabinose (Ara), xylose (Xyl), and mannose (Man), at a molar ratio of 51.4꞉15.5꞉6.0꞉11.8꞉4.2꞉4.4꞉4.2. There were two regions of AGA (70%) and rhamnogalacturonan-I (RG-Ι, 30%) in ZCMP. AGA was composed of an α-1,4-D-galactopyranosyluronan backbone mainly substituted at the O-3 position by single Api residues. RG-Ι possessed a backbone of repeating disaccharide units of →4GalAα1,2Rhaα1→, with a few α-L-arabinose and β-D-galactose residues as side chains. The anti-angiogenesis assay showed that ZCMP inhibited the migratory activity of human umbilical vein endothelial cell (HUVECs), with no influence on endothelial cells growth. ZCMP also promoted macrophage phagocytosis. These findings of the present study demonstrated the potential anti-tumor activity of ZCMP through anti-angiogenic and immunoregulatory pathways.

No MeSH data available.


Related in: MedlinePlus