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White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing.

Chen YY, Chen JC, Lin YC, Yeh ST, Huang CL - Mar Drugs (2015)

Bottom Line: The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not.In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress.Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PX), cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp.

View Article: PubMed Central - PubMed

Affiliation: Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung 202, Taiwan. fivedollarboy555@yahoo.com.tw.

ABSTRACT
White shrimp Litopenaeus vannamei immersed in seawater (35‰) containing Gracilaria tenuistipitata extract (GTE) at 0 (control), 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen), and immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total hemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PX), cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters.

No MeSH data available.


(A) Phenoloxidase activity; (B) respiratory burst (RB); and (C) superoxide dismutase (SOD) activity of white shrimp Litopenaeus vannamei immersed in seawater containing Gracilaria tenuistipitata extract at 0 (control), 400, and 600 mg/L for 3 h and then exposed to 5 mg/L ammonia-N for 24, 48, 72, 96, and 120 h. Data (mean ± SE) with different letters are significantly different (p < 0.05) among treatments
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marinedrugs-13-03606-f002: (A) Phenoloxidase activity; (B) respiratory burst (RB); and (C) superoxide dismutase (SOD) activity of white shrimp Litopenaeus vannamei immersed in seawater containing Gracilaria tenuistipitata extract at 0 (control), 400, and 600 mg/L for 3 h and then exposed to 5 mg/L ammonia-N for 24, 48, 72, 96, and 120 h. Data (mean ± SE) with different letters are significantly different (p < 0.05) among treatments

Mentions: Prior to the ammonia stress test, immune parameters increased directly with concentration of GTE. The levels of HC, GC, and THC of shrimp immersed in seawater containing 400 mg/L GTE increased by 38%, 33%, and 37%, respectively. PO activity, RB, SOD activity, lysozyme activity, and hemolymph protein level of shrimp immersed in 400 mg/L GTE increased significantly by 43%, 39%, 47%, 79%, and 36%, respectively (Figure 1, Figure 2 and Figure 3).


White Shrimp Litopenaeus vannamei That Have Received Gracilaria tenuistipitata Extract Show Early Recovery of Immune Parameters after Ammonia Stressing.

Chen YY, Chen JC, Lin YC, Yeh ST, Huang CL - Mar Drugs (2015)

(A) Phenoloxidase activity; (B) respiratory burst (RB); and (C) superoxide dismutase (SOD) activity of white shrimp Litopenaeus vannamei immersed in seawater containing Gracilaria tenuistipitata extract at 0 (control), 400, and 600 mg/L for 3 h and then exposed to 5 mg/L ammonia-N for 24, 48, 72, 96, and 120 h. Data (mean ± SE) with different letters are significantly different (p < 0.05) among treatments
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483647&req=5

marinedrugs-13-03606-f002: (A) Phenoloxidase activity; (B) respiratory burst (RB); and (C) superoxide dismutase (SOD) activity of white shrimp Litopenaeus vannamei immersed in seawater containing Gracilaria tenuistipitata extract at 0 (control), 400, and 600 mg/L for 3 h and then exposed to 5 mg/L ammonia-N for 24, 48, 72, 96, and 120 h. Data (mean ± SE) with different letters are significantly different (p < 0.05) among treatments
Mentions: Prior to the ammonia stress test, immune parameters increased directly with concentration of GTE. The levels of HC, GC, and THC of shrimp immersed in seawater containing 400 mg/L GTE increased by 38%, 33%, and 37%, respectively. PO activity, RB, SOD activity, lysozyme activity, and hemolymph protein level of shrimp immersed in 400 mg/L GTE increased significantly by 43%, 39%, 47%, 79%, and 36%, respectively (Figure 1, Figure 2 and Figure 3).

Bottom Line: The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not.In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress.Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PX), cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp.

View Article: PubMed Central - PubMed

Affiliation: Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung 202, Taiwan. fivedollarboy555@yahoo.com.tw.

ABSTRACT
White shrimp Litopenaeus vannamei immersed in seawater (35‰) containing Gracilaria tenuistipitata extract (GTE) at 0 (control), 400, and 600 mg/L for 3 h were exposed to 5 mg/L ammonia-N (ammonia as nitrogen), and immune parameters including hyaline cells (HCs), granular cells (GCs, including semi-granular cells), total hemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (RBs), superoxide dismutase (SOD) activity, lysozyme activity, and hemolymph protein level were examined 24~120 h post-stress. The immune parameters of shrimp immersed in 600 mg/L GTE returned to original values earlier, at 96~120 h post-stress, whereas in control shrimp they did not. In another experiment, shrimp were immersed in seawater containing GTE at 0 and 600 mg/L for 3 h and examined for transcript levels of immune-related genes at 24 h post-stress. Transcript levels of lipopolysaccharide and β-1,3-glucan binding protein (LGBP), peroxinectin (PX), cytMnSOD, mtMnSOD, and HSP70 were up-regulated at 24 h post-stress in GTE receiving shrimp. We concluded that white shrimp immersed in seawater containing GTE exhibited a capability for maintaining homeostasis by regulating cellular and humoral immunity against ammonia stress as evidenced by up-regulated gene expression and earlier recovery of immune parameters.

No MeSH data available.