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Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens.

Busetti A, Thompson TP, Tegazzini D, Megaw J, Maggs CA, Gilmore BF - Mar Drugs (2015)

Bottom Line: Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively.Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay.A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, BT9 7BL Belfast, UK. a.busetti@qub.ac.uk.

ABSTRACT
The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

No MeSH data available.


Related in: MedlinePlus

Screening fresh H. siliquosa fronds for antimicrobial, QSI and QS activity using the overlay method. (A) Very weak antimicrobial activity (2 mm inhibition zone) against E. coli ATCC 11303; (B) No antimicrobial activity detected against K. pneumonia NCTC 204; (C) Pronounced antimicrobial activity of H. siliquosa against S. aureus (MRSA) ATCC 33595; (D,E) Weak antimicrobial activity of H. siliquosa against P. aeruginosa PAO1 and PA14; (F) No antimicrobial nor QSI activity detected against Serratia sp. ATCC39006; (G) No antimicrobial activity against E. cloacae. (H,I) Pronounced antimicrobial activity of H. siliquosa against QSI reporter strain C. violaceum ATCC 12472 and QS reporter strain C. violaceum CV026.
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marinedrugs-13-03581-f005: Screening fresh H. siliquosa fronds for antimicrobial, QSI and QS activity using the overlay method. (A) Very weak antimicrobial activity (2 mm inhibition zone) against E. coli ATCC 11303; (B) No antimicrobial activity detected against K. pneumonia NCTC 204; (C) Pronounced antimicrobial activity of H. siliquosa against S. aureus (MRSA) ATCC 33595; (D,E) Weak antimicrobial activity of H. siliquosa against P. aeruginosa PAO1 and PA14; (F) No antimicrobial nor QSI activity detected against Serratia sp. ATCC39006; (G) No antimicrobial activity against E. cloacae. (H,I) Pronounced antimicrobial activity of H. siliquosa against QSI reporter strain C. violaceum ATCC 12472 and QS reporter strain C. violaceum CV026.

Mentions: The overlay method [65] was used to screen fresh algal fronds for antimicrobial activity against E. coli ATCC 11303, P. aeruginosa PAO1, P. aeruginosa PA14, E. cloacae, E. faecium DSM 25390, S. aureus MRSA ATCC 33593, and K. pneumonia 204. Marked antimicrobial activity was observed against S. aureus MRSA ATCC 33593 and the two QS-reporter strains C. violaceum ATCC 12472 and C. violaceum CV026 and weak antimicrobial activity was observed against P. aeruginosa PAO1 and PA14 (Figure 5). A very weak antimicrobial activity was detected against E. coli ATCC 11303 consisting in an inhibition zone of approximately 2 mm surrounding the fresh algal sample. The pronounced antimicrobial activity displayed by fresh H. siliquosa fronds against reporter strains C. violaceum ATCC 12472 and CV026 prevented the detection of QSI or QS compounds using these two reporter strains. The overlay of fresh H. siliquosa fronds using QSI reporter Serratia sp. ATCC 39006 failed to detect relevant QSI or antimicrobial activity against this strain.


Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens.

Busetti A, Thompson TP, Tegazzini D, Megaw J, Maggs CA, Gilmore BF - Mar Drugs (2015)

Screening fresh H. siliquosa fronds for antimicrobial, QSI and QS activity using the overlay method. (A) Very weak antimicrobial activity (2 mm inhibition zone) against E. coli ATCC 11303; (B) No antimicrobial activity detected against K. pneumonia NCTC 204; (C) Pronounced antimicrobial activity of H. siliquosa against S. aureus (MRSA) ATCC 33595; (D,E) Weak antimicrobial activity of H. siliquosa against P. aeruginosa PAO1 and PA14; (F) No antimicrobial nor QSI activity detected against Serratia sp. ATCC39006; (G) No antimicrobial activity against E. cloacae. (H,I) Pronounced antimicrobial activity of H. siliquosa against QSI reporter strain C. violaceum ATCC 12472 and QS reporter strain C. violaceum CV026.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4483646&req=5

marinedrugs-13-03581-f005: Screening fresh H. siliquosa fronds for antimicrobial, QSI and QS activity using the overlay method. (A) Very weak antimicrobial activity (2 mm inhibition zone) against E. coli ATCC 11303; (B) No antimicrobial activity detected against K. pneumonia NCTC 204; (C) Pronounced antimicrobial activity of H. siliquosa against S. aureus (MRSA) ATCC 33595; (D,E) Weak antimicrobial activity of H. siliquosa against P. aeruginosa PAO1 and PA14; (F) No antimicrobial nor QSI activity detected against Serratia sp. ATCC39006; (G) No antimicrobial activity against E. cloacae. (H,I) Pronounced antimicrobial activity of H. siliquosa against QSI reporter strain C. violaceum ATCC 12472 and QS reporter strain C. violaceum CV026.
Mentions: The overlay method [65] was used to screen fresh algal fronds for antimicrobial activity against E. coli ATCC 11303, P. aeruginosa PAO1, P. aeruginosa PA14, E. cloacae, E. faecium DSM 25390, S. aureus MRSA ATCC 33593, and K. pneumonia 204. Marked antimicrobial activity was observed against S. aureus MRSA ATCC 33593 and the two QS-reporter strains C. violaceum ATCC 12472 and C. violaceum CV026 and weak antimicrobial activity was observed against P. aeruginosa PAO1 and PA14 (Figure 5). A very weak antimicrobial activity was detected against E. coli ATCC 11303 consisting in an inhibition zone of approximately 2 mm surrounding the fresh algal sample. The pronounced antimicrobial activity displayed by fresh H. siliquosa fronds against reporter strains C. violaceum ATCC 12472 and CV026 prevented the detection of QSI or QS compounds using these two reporter strains. The overlay of fresh H. siliquosa fronds using QSI reporter Serratia sp. ATCC 39006 failed to detect relevant QSI or antimicrobial activity against this strain.

Bottom Line: Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively.Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay.A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, BT9 7BL Belfast, UK. a.busetti@qub.ac.uk.

ABSTRACT
The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

No MeSH data available.


Related in: MedlinePlus