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Biological Activities and Chemical Composition of Methanolic Extracts of Selected Autochthonous Microalgae Strains from the Red Sea.

Pereira H, Custódio L, Rodrigues MJ, de Sousa CB, Oliveira M, Barreira L, Neng Nda R, Nogueira JM, Alrokayan SA, Mouffouk F, Abu-Salah KM, Ben-Hamadou R, Varela J - Mar Drugs (2015)

Bottom Line: SBL4 extracts significantly reduced the viability of tumoral (HepG2 and HeLa) cells with lower toxicity against the non-tumoral murine stromal (S17) cells.SBL1 significantly reduced the viability of Leishmania infantum down to 62% (250 µg/mL).SBL2.

View Article: PubMed Central - PubMed

Affiliation: Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal. hgpereira@ualg.pt.

ABSTRACT
Four lipid-rich microalgal species from the Red Sea belonging to three different genera (Nannochloris, Picochlorum and Desmochloris), previously isolated as novel biodiesel feedstocks, were bioprospected for high-value, bioactive molecules. Methanol extracts were thus prepared from freeze-dried biomass and screened for different biological activities. Nannochloris sp. SBL1 and Desmochloris sp. SBL3 had the highest radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl, and the best copper and iron chelating activities. All species had potent butyrylcholinesterase inhibitory activity (>50%) and mildly inhibited tyrosinase. Picochlorum sp. SBL2 and Nannochloris sp. SBL4 extracts significantly reduced the viability of tumoral (HepG2 and HeLa) cells with lower toxicity against the non-tumoral murine stromal (S17) cells. Nannochloris sp. SBL1 significantly reduced the viability of Leishmania infantum down to 62% (250 µg/mL). Picochlorum sp. SBL2 had the highest total phenolic content, the major phenolic compounds identified being salicylic, coumaric and gallic acids. Neoxanthin, violaxanthin, zeaxanthin, lutein and β-carotene were identified in the extracts of all strains, while canthaxanthin was only identified in Picochlorum sp. SBL2. Taken together, these results strongly suggest that the microalgae included in this work could be used as sources of added-value products that could be used to upgrade the final biomass value.

No MeSH data available.


Related in: MedlinePlus

Effect of the application of methanol extracts of four strains belonging to the Nannochloris (SBL1 and SBL4), Picochlorum (SBL2) and Desmochloris (SBL3) genera, applied at a concentration of 125 µg/mL for 72 h, on the viability of human hepatocarcinoma (HepG2), cervical carcinoma (HeLa) and non-tumoral murine stromal (S17) cell lines, in comparison to a control without extract (DMSO, 0.5%, v/v). Bars and lines correspond, respectively, to cell viability and selectivity. * Significant differences (p < 0.001) compared with the control (n = 6). Half maximal inhibitory concentrations for etoposide used as a positive control were 1.9, 4.2 and 10 µg/mL for HepG2, HeLa and S17 cells, respectively.
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marinedrugs-13-03531-f002: Effect of the application of methanol extracts of four strains belonging to the Nannochloris (SBL1 and SBL4), Picochlorum (SBL2) and Desmochloris (SBL3) genera, applied at a concentration of 125 µg/mL for 72 h, on the viability of human hepatocarcinoma (HepG2), cervical carcinoma (HeLa) and non-tumoral murine stromal (S17) cell lines, in comparison to a control without extract (DMSO, 0.5%, v/v). Bars and lines correspond, respectively, to cell viability and selectivity. * Significant differences (p < 0.001) compared with the control (n = 6). Half maximal inhibitory concentrations for etoposide used as a positive control were 1.9, 4.2 and 10 µg/mL for HepG2, HeLa and S17 cells, respectively.

Mentions: According to the World Health Organization (WHO), cancer is responsible for about 13% (~7.6 million) of fatalities worldwide, being the second most common cause of death from disease after myocardial infarction. The current available antitumoral drugs generally display undesirable effects, making the search for more effective and safer drugs necessary. The algal extracts under study were thus tested against two human tumoral cell lines, namely: HepG2 (hepatocellular carcinoma) and HeLa (cervical carcinoma). Samples were applied for 72 h at a concentration of 125 µg/mL, and cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay [30]. To evaluate selectivity, samples were applied to a murine non-tumoral cell line (S17, stromal cells). None of the extracts were cytotoxic to non-tumoral cells and displayed different degrees of toxicity towards tumoral cell lines (Figure 2). Nannochloris sp. SBL1 and Desmochloris sp. SBL3 did not reduce significantly the viability of any of the cell lines tested, suggesting a nontoxic nature for the compounds present in the extract of those species. Extracts from Picochlorum sp. SBL2 and Nannochloris sp. SBL4 significantly reduced the viability of both HepG2 and HeLa cells, with lower toxicity against non-tumoral S17 cells. However, Nannochloris sp. SBL4 extracts showed the highest selectivity index (SI), suggesting that this strain contains molecules with interesting antitumoral properties that may act selectively on cancer cells. Although microalgae have long been recognized as sources of important biomolecules with potential medical uses [31], there have been few reports on their cytotoxicity against human tumoral cells [32,33].


Biological Activities and Chemical Composition of Methanolic Extracts of Selected Autochthonous Microalgae Strains from the Red Sea.

Pereira H, Custódio L, Rodrigues MJ, de Sousa CB, Oliveira M, Barreira L, Neng Nda R, Nogueira JM, Alrokayan SA, Mouffouk F, Abu-Salah KM, Ben-Hamadou R, Varela J - Mar Drugs (2015)

Effect of the application of methanol extracts of four strains belonging to the Nannochloris (SBL1 and SBL4), Picochlorum (SBL2) and Desmochloris (SBL3) genera, applied at a concentration of 125 µg/mL for 72 h, on the viability of human hepatocarcinoma (HepG2), cervical carcinoma (HeLa) and non-tumoral murine stromal (S17) cell lines, in comparison to a control without extract (DMSO, 0.5%, v/v). Bars and lines correspond, respectively, to cell viability and selectivity. * Significant differences (p < 0.001) compared with the control (n = 6). Half maximal inhibitory concentrations for etoposide used as a positive control were 1.9, 4.2 and 10 µg/mL for HepG2, HeLa and S17 cells, respectively.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4483643&req=5

marinedrugs-13-03531-f002: Effect of the application of methanol extracts of four strains belonging to the Nannochloris (SBL1 and SBL4), Picochlorum (SBL2) and Desmochloris (SBL3) genera, applied at a concentration of 125 µg/mL for 72 h, on the viability of human hepatocarcinoma (HepG2), cervical carcinoma (HeLa) and non-tumoral murine stromal (S17) cell lines, in comparison to a control without extract (DMSO, 0.5%, v/v). Bars and lines correspond, respectively, to cell viability and selectivity. * Significant differences (p < 0.001) compared with the control (n = 6). Half maximal inhibitory concentrations for etoposide used as a positive control were 1.9, 4.2 and 10 µg/mL for HepG2, HeLa and S17 cells, respectively.
Mentions: According to the World Health Organization (WHO), cancer is responsible for about 13% (~7.6 million) of fatalities worldwide, being the second most common cause of death from disease after myocardial infarction. The current available antitumoral drugs generally display undesirable effects, making the search for more effective and safer drugs necessary. The algal extracts under study were thus tested against two human tumoral cell lines, namely: HepG2 (hepatocellular carcinoma) and HeLa (cervical carcinoma). Samples were applied for 72 h at a concentration of 125 µg/mL, and cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay [30]. To evaluate selectivity, samples were applied to a murine non-tumoral cell line (S17, stromal cells). None of the extracts were cytotoxic to non-tumoral cells and displayed different degrees of toxicity towards tumoral cell lines (Figure 2). Nannochloris sp. SBL1 and Desmochloris sp. SBL3 did not reduce significantly the viability of any of the cell lines tested, suggesting a nontoxic nature for the compounds present in the extract of those species. Extracts from Picochlorum sp. SBL2 and Nannochloris sp. SBL4 significantly reduced the viability of both HepG2 and HeLa cells, with lower toxicity against non-tumoral S17 cells. However, Nannochloris sp. SBL4 extracts showed the highest selectivity index (SI), suggesting that this strain contains molecules with interesting antitumoral properties that may act selectively on cancer cells. Although microalgae have long been recognized as sources of important biomolecules with potential medical uses [31], there have been few reports on their cytotoxicity against human tumoral cells [32,33].

Bottom Line: SBL4 extracts significantly reduced the viability of tumoral (HepG2 and HeLa) cells with lower toxicity against the non-tumoral murine stromal (S17) cells.SBL1 significantly reduced the viability of Leishmania infantum down to 62% (250 µg/mL).SBL2.

View Article: PubMed Central - PubMed

Affiliation: Centre of Marine Sciences, University of Algarve, Faro 8005-139, Portugal. hgpereira@ualg.pt.

ABSTRACT
Four lipid-rich microalgal species from the Red Sea belonging to three different genera (Nannochloris, Picochlorum and Desmochloris), previously isolated as novel biodiesel feedstocks, were bioprospected for high-value, bioactive molecules. Methanol extracts were thus prepared from freeze-dried biomass and screened for different biological activities. Nannochloris sp. SBL1 and Desmochloris sp. SBL3 had the highest radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl, and the best copper and iron chelating activities. All species had potent butyrylcholinesterase inhibitory activity (>50%) and mildly inhibited tyrosinase. Picochlorum sp. SBL2 and Nannochloris sp. SBL4 extracts significantly reduced the viability of tumoral (HepG2 and HeLa) cells with lower toxicity against the non-tumoral murine stromal (S17) cells. Nannochloris sp. SBL1 significantly reduced the viability of Leishmania infantum down to 62% (250 µg/mL). Picochlorum sp. SBL2 had the highest total phenolic content, the major phenolic compounds identified being salicylic, coumaric and gallic acids. Neoxanthin, violaxanthin, zeaxanthin, lutein and β-carotene were identified in the extracts of all strains, while canthaxanthin was only identified in Picochlorum sp. SBL2. Taken together, these results strongly suggest that the microalgae included in this work could be used as sources of added-value products that could be used to upgrade the final biomass value.

No MeSH data available.


Related in: MedlinePlus