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The antihyperlipidemic mechanism of high sulfate content ulvan in rats.

Qi H, Sheng J - Mar Drugs (2015)

Bottom Line: Moreover, some HU groups could upregulate the mRNA expression of FXR and PPARγ and downregulate the expression of LXR.All HU female groups could upregulate the mRNA expression of PPARγ in a concentration-dependent manner.In summary, HU could improve lipid profiles through upregulation of FXR and PPARγ and downregulation of LXR.

View Article: PubMed Central - PubMed

Affiliation: Weifang Medical University, No.7166 Baotong Road, Weifang 261053, China. wfqihuimin@126.com.

ABSTRACT
Numerous studies have suggested that hyperlipidemia is closely linked to cardiovascular disease. The aim of this study was to investigate the possible antihyperlipidemia mechanism of HU (high sulfate content of ulvan) in high-cholesterol fed rats. Wistar rats were made hyperlipidemic by feeding with a high-cholesterol diet. HU was administered to these hyperlipidemia rats for 30 days. Lipid levels and the mRNA expressions of FXR, LXR and PPARγ in liver were measured after 30 days of treatment. In the HU-treated groups, the middle dosage group of male rats (total cholesterol (TC): p < 0.01) and the low-dosage group of female rats (TC, LDL-C: p < 0.01) showed stronger activity with respect to antihyperlipidemia. Moreover, some HU groups could upregulate the mRNA expression of FXR and PPARγ and downregulate the expression of LXR. For the male rats, compared with the hyperlipidemia group, the middle dosage HU had the most pronounced effect on increasing the mRNA levels of FXR (p < 0.01); low- and high-dosage HU showed a significant inhibition of the mRNA levels of LXR (p < 0.01). All HU female groups could upregulate the mRNA expression of PPARγ in a concentration-dependent manner. In summary, HU could improve lipid profiles through upregulation of FXR and PPARγ and downregulation of LXR.

No MeSH data available.


Related in: MedlinePlus

The structure of ulvan, the main disaccharide units [β-d-Glcp A-(1→4)-α-l-Rhap 3s] and [α-l-Idop A-(1→4)-α-l-Rhap 3s]; G: (1→4)-linked β-d-glucuronic acid; R: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with β-d-glucuronic acid); I: (1→4)-linked α-l-iduronic acid; R*: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with α-l-iduronic acid).
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marinedrugs-13-03407-f001: The structure of ulvan, the main disaccharide units [β-d-Glcp A-(1→4)-α-l-Rhap 3s] and [α-l-Idop A-(1→4)-α-l-Rhap 3s]; G: (1→4)-linked β-d-glucuronic acid; R: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with β-d-glucuronic acid); I: (1→4)-linked α-l-iduronic acid; R*: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with α-l-iduronic acid).

Mentions: The green alga, Ulva pertusa, is nutritious, low calorie and abundant in vitamins, trace elements and dietary fibers [10]. Moreover, it is an important marine drug, prescribed in the Chinese Marine Materia Medica [11]. Ulvan, the sulfated polysaccharide comprising the hot-water soluble portion of the cell wall, is one of the main components of U. pertusa, and the main disaccharide units are [β-d-GlcpA-(1→4)-α-l-Rhap3s] and [α-l-Idop A-(1→4)-α-l-Rhap 3s]. The main structure of ulvan is shown in Figure 1 [12]. During the last few years, ulvan has been reported to have antihyperlipidemic, antioxidant, antitumor and antiviral activities [12,13,14,15]. The derivative of high sulfate content ulvan (HU) was prepared and showed higher antihyperlipidemic activity than ulvan [9]. However, there is no information on the antihyperlipidemic mechanism of HU so far. Thus, the present investigation aimed to determine the expression of lipid metabolism genes, including FXR, LXR and PPARγ, in rat’s livers, then tried to prove the antihyperlipidemic mechanism of HU.


The antihyperlipidemic mechanism of high sulfate content ulvan in rats.

Qi H, Sheng J - Mar Drugs (2015)

The structure of ulvan, the main disaccharide units [β-d-Glcp A-(1→4)-α-l-Rhap 3s] and [α-l-Idop A-(1→4)-α-l-Rhap 3s]; G: (1→4)-linked β-d-glucuronic acid; R: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with β-d-glucuronic acid); I: (1→4)-linked α-l-iduronic acid; R*: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with α-l-iduronic acid).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483636&req=5

marinedrugs-13-03407-f001: The structure of ulvan, the main disaccharide units [β-d-Glcp A-(1→4)-α-l-Rhap 3s] and [α-l-Idop A-(1→4)-α-l-Rhap 3s]; G: (1→4)-linked β-d-glucuronic acid; R: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with β-d-glucuronic acid); I: (1→4)-linked α-l-iduronic acid; R*: (1→4)-linked-α-l-rhamnose-3-sulfate (linked with α-l-iduronic acid).
Mentions: The green alga, Ulva pertusa, is nutritious, low calorie and abundant in vitamins, trace elements and dietary fibers [10]. Moreover, it is an important marine drug, prescribed in the Chinese Marine Materia Medica [11]. Ulvan, the sulfated polysaccharide comprising the hot-water soluble portion of the cell wall, is one of the main components of U. pertusa, and the main disaccharide units are [β-d-GlcpA-(1→4)-α-l-Rhap3s] and [α-l-Idop A-(1→4)-α-l-Rhap 3s]. The main structure of ulvan is shown in Figure 1 [12]. During the last few years, ulvan has been reported to have antihyperlipidemic, antioxidant, antitumor and antiviral activities [12,13,14,15]. The derivative of high sulfate content ulvan (HU) was prepared and showed higher antihyperlipidemic activity than ulvan [9]. However, there is no information on the antihyperlipidemic mechanism of HU so far. Thus, the present investigation aimed to determine the expression of lipid metabolism genes, including FXR, LXR and PPARγ, in rat’s livers, then tried to prove the antihyperlipidemic mechanism of HU.

Bottom Line: Moreover, some HU groups could upregulate the mRNA expression of FXR and PPARγ and downregulate the expression of LXR.All HU female groups could upregulate the mRNA expression of PPARγ in a concentration-dependent manner.In summary, HU could improve lipid profiles through upregulation of FXR and PPARγ and downregulation of LXR.

View Article: PubMed Central - PubMed

Affiliation: Weifang Medical University, No.7166 Baotong Road, Weifang 261053, China. wfqihuimin@126.com.

ABSTRACT
Numerous studies have suggested that hyperlipidemia is closely linked to cardiovascular disease. The aim of this study was to investigate the possible antihyperlipidemia mechanism of HU (high sulfate content of ulvan) in high-cholesterol fed rats. Wistar rats were made hyperlipidemic by feeding with a high-cholesterol diet. HU was administered to these hyperlipidemia rats for 30 days. Lipid levels and the mRNA expressions of FXR, LXR and PPARγ in liver were measured after 30 days of treatment. In the HU-treated groups, the middle dosage group of male rats (total cholesterol (TC): p < 0.01) and the low-dosage group of female rats (TC, LDL-C: p < 0.01) showed stronger activity with respect to antihyperlipidemia. Moreover, some HU groups could upregulate the mRNA expression of FXR and PPARγ and downregulate the expression of LXR. For the male rats, compared with the hyperlipidemia group, the middle dosage HU had the most pronounced effect on increasing the mRNA levels of FXR (p < 0.01); low- and high-dosage HU showed a significant inhibition of the mRNA levels of LXR (p < 0.01). All HU female groups could upregulate the mRNA expression of PPARγ in a concentration-dependent manner. In summary, HU could improve lipid profiles through upregulation of FXR and PPARγ and downregulation of LXR.

No MeSH data available.


Related in: MedlinePlus