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Production of Chondroitin Sulphate from Head, Skeleton and Fins of Scyliorhinus canicula By-Products by Combination of Enzymatic, Chemical Precipitation and Ultrafiltration Methodologies.

Blanco M, Fraguas J, Sotelo CG, Pérez-Martín RI, Vázquez JA - Mar Drugs (2015)

Bottom Line: Then, similar optimal conditions were observed for skeletons and fin materials.Enzymatic hydrolysates were subsequently treated with a combination of alkaline hydroalcoholic saline solutions in order to improve the protein hydrolysis and the selective precipitation of CS.Ranges of 0.53-0.64 M (NaOH) and 1.14-1.20 volumes (EtOH) were the levels for optimal chemical treatment depending on the cartilage origin.

View Article: PubMed Central - PubMed

Affiliation: Marine Research Institute (IIM-CSIC), Eduardo Cabello, 6. Vigo, Galicia 36208, Spain. mblanco@iim.csic.es.

ABSTRACT
This study illustrates the optimisation of the experimental conditions of three sequential steps for chondroitin sulphate (CS) recovery from three cartilaginous materials of Scyliorhinus canicula by-products. Optimum conditions of temperature and pH were first obtained for alcalase proteolysis of head cartilage (58 °C/pH 8.5/0.1% (v/w)/10 h of hydrolysis). Then, similar optimal conditions were observed for skeletons and fin materials. Enzymatic hydrolysates were subsequently treated with a combination of alkaline hydroalcoholic saline solutions in order to improve the protein hydrolysis and the selective precipitation of CS. Ranges of 0.53-0.64 M (NaOH) and 1.14-1.20 volumes (EtOH) were the levels for optimal chemical treatment depending on the cartilage origin. Finally, selective purification and concentration of CS and protein elimination of samples obtained from chemical treatment, was assessed by a combination of ultrafiltration and diafiltration (UF-DF) techniques at 30 kDa.

No MeSH data available.


Predicted response surfaces by empirical equations summarized in Table 7 corresponding to the combined effect of NaOH and EtOH on the selective treatment of CS from hydrolysate cartilages of S. canicula.
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marinedrugs-13-03287-f004: Predicted response surfaces by empirical equations summarized in Table 7 corresponding to the combined effect of NaOH and EtOH on the selective treatment of CS from hydrolysate cartilages of S. canicula.

Mentions: The adjusted coefficients of determination were higher than 0.83 indicating a good correlation between experimental data and theoretical responses. In all cases, responses were significantly affected by positive E and N linear terms and negative quadratic coefficients of both variables (p <0.05). The predicted response surfaces were very homogeneous displaying perfect domes (convex surfaces) in the experimental domain executed (Figure 4). Nevertheless, cases of over and under-estimation were observed (Table 6), which do not invalidate the results, and are due to not achieving coefficients of determination nearer to one (Table 7). As described previously, the present values revealed good but not perfect agreement among surfaces and experimental data; therefore little lack of fit is commonly obtained.


Production of Chondroitin Sulphate from Head, Skeleton and Fins of Scyliorhinus canicula By-Products by Combination of Enzymatic, Chemical Precipitation and Ultrafiltration Methodologies.

Blanco M, Fraguas J, Sotelo CG, Pérez-Martín RI, Vázquez JA - Mar Drugs (2015)

Predicted response surfaces by empirical equations summarized in Table 7 corresponding to the combined effect of NaOH and EtOH on the selective treatment of CS from hydrolysate cartilages of S. canicula.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4483629&req=5

marinedrugs-13-03287-f004: Predicted response surfaces by empirical equations summarized in Table 7 corresponding to the combined effect of NaOH and EtOH on the selective treatment of CS from hydrolysate cartilages of S. canicula.
Mentions: The adjusted coefficients of determination were higher than 0.83 indicating a good correlation between experimental data and theoretical responses. In all cases, responses were significantly affected by positive E and N linear terms and negative quadratic coefficients of both variables (p <0.05). The predicted response surfaces were very homogeneous displaying perfect domes (convex surfaces) in the experimental domain executed (Figure 4). Nevertheless, cases of over and under-estimation were observed (Table 6), which do not invalidate the results, and are due to not achieving coefficients of determination nearer to one (Table 7). As described previously, the present values revealed good but not perfect agreement among surfaces and experimental data; therefore little lack of fit is commonly obtained.

Bottom Line: Then, similar optimal conditions were observed for skeletons and fin materials.Enzymatic hydrolysates were subsequently treated with a combination of alkaline hydroalcoholic saline solutions in order to improve the protein hydrolysis and the selective precipitation of CS.Ranges of 0.53-0.64 M (NaOH) and 1.14-1.20 volumes (EtOH) were the levels for optimal chemical treatment depending on the cartilage origin.

View Article: PubMed Central - PubMed

Affiliation: Marine Research Institute (IIM-CSIC), Eduardo Cabello, 6. Vigo, Galicia 36208, Spain. mblanco@iim.csic.es.

ABSTRACT
This study illustrates the optimisation of the experimental conditions of three sequential steps for chondroitin sulphate (CS) recovery from three cartilaginous materials of Scyliorhinus canicula by-products. Optimum conditions of temperature and pH were first obtained for alcalase proteolysis of head cartilage (58 °C/pH 8.5/0.1% (v/w)/10 h of hydrolysis). Then, similar optimal conditions were observed for skeletons and fin materials. Enzymatic hydrolysates were subsequently treated with a combination of alkaline hydroalcoholic saline solutions in order to improve the protein hydrolysis and the selective precipitation of CS. Ranges of 0.53-0.64 M (NaOH) and 1.14-1.20 volumes (EtOH) were the levels for optimal chemical treatment depending on the cartilage origin. Finally, selective purification and concentration of CS and protein elimination of samples obtained from chemical treatment, was assessed by a combination of ultrafiltration and diafiltration (UF-DF) techniques at 30 kDa.

No MeSH data available.