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Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus

Alterations in biological pathways in the mutant revealed by GO analysis (arrows in the upper and lower direction indicate pathways induced by up-regulated and down-regulated URDEGs, respectively; pathways given in the left, right and middle are altered under control, stress and also both control and stress conditions, respectively).
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PLV023F8: Alterations in biological pathways in the mutant revealed by GO analysis (arrows in the upper and lower direction indicate pathways induced by up-regulated and down-regulated URDEGs, respectively; pathways given in the left, right and middle are altered under control, stress and also both control and stress conditions, respectively).

Mentions: Functional GO annotations of the identified URDEGs were analysed in terms of GO enrichments for biological, molecular and cellular functions. Under control conditions, biological GO terms of carboxylic acid metabolic process (GO:0019752), protein phosphorylation (GO:0006468) and exocytosis (GO:0006887) were enriched, whereas under stress conditions, genes for tryptophan biosynthesis (GO:0000162), lignin biosynthesis (GO:0009809) and iron ion transport (GO:0006826) were enriched. The GO terms of flavonoid biosynthesis (GO:0009813), phenylpropanoid metabolic process (GO:0009698) and l-phenylalanine catabolic process (GO:0006559) were enriched in the set of up-regulated URDEGs in both control and stress samples. No significant biological function GO term enrichment could be seen in the set of down-regulated genes in ewst1 either under control or stress conditions. The molecular function GO enrichment analysis of up-regulated URDEGs revealed genes involved in ATP binding (GO:0005524), metal ion binding (GO:0046872), intramolecular lyase activity (GO:0016872), peroxiredoxin activity (GO:0051920), protein serine/threonine kinase activity (GO:0004674) and phosphoheptulonate synthase activity (GO:0003849) under control conditions, whereas genes representing peroxidase activity (GO:0004601), cinamoyl alcohol dehydrogenase (GO:0045551), anthranilate phosphoribosyl transferase (GO:0004048), indole-3-glycerol phosphate synthase (GO:0004425), heme (GO:0020037) and ferric ion binding (GO:0008199) were significantly enriched under stress in ewst1. The URDEGs for DNA binding (GO:0003677), Zinc ion binding (GO:0008270) and oxidoreductase (GO:0016702) were down-regulated in ewst1 under control. Interestingly, there was only one GO term, phosphoglycolate phosphatase (GO:0008967), that was significantly enriched among the down-regulated genes under stress. However, in cellular function GO enrichment analysis, GO terms like exocyst (GO:0000145), intracellular membrane bounded organelle (GO:0043231), membrane (GO:0016020) and extracellular region (GO:0005576) were found to be significant in the up-regulated gene set, whereas nucleus (GO:0005634) GO term was enriched in the down-regulated gene set of URDEGs in control conditions. The overview of GO enrichment analysis is depicted in Fig. 8. Pathway analysis of URDEGs indicated that there were significant expressional alterations in flavonoid biosynthesis, phenylpropanoid biosynthesis, starch and sucrose metabolism and tryptophan biosynthesis-related genes in ewst1 when compared with WT.Figure 8.


Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Alterations in biological pathways in the mutant revealed by GO analysis (arrows in the upper and lower direction indicate pathways induced by up-regulated and down-regulated URDEGs, respectively; pathways given in the left, right and middle are altered under control, stress and also both control and stress conditions, respectively).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482838&req=5

PLV023F8: Alterations in biological pathways in the mutant revealed by GO analysis (arrows in the upper and lower direction indicate pathways induced by up-regulated and down-regulated URDEGs, respectively; pathways given in the left, right and middle are altered under control, stress and also both control and stress conditions, respectively).
Mentions: Functional GO annotations of the identified URDEGs were analysed in terms of GO enrichments for biological, molecular and cellular functions. Under control conditions, biological GO terms of carboxylic acid metabolic process (GO:0019752), protein phosphorylation (GO:0006468) and exocytosis (GO:0006887) were enriched, whereas under stress conditions, genes for tryptophan biosynthesis (GO:0000162), lignin biosynthesis (GO:0009809) and iron ion transport (GO:0006826) were enriched. The GO terms of flavonoid biosynthesis (GO:0009813), phenylpropanoid metabolic process (GO:0009698) and l-phenylalanine catabolic process (GO:0006559) were enriched in the set of up-regulated URDEGs in both control and stress samples. No significant biological function GO term enrichment could be seen in the set of down-regulated genes in ewst1 either under control or stress conditions. The molecular function GO enrichment analysis of up-regulated URDEGs revealed genes involved in ATP binding (GO:0005524), metal ion binding (GO:0046872), intramolecular lyase activity (GO:0016872), peroxiredoxin activity (GO:0051920), protein serine/threonine kinase activity (GO:0004674) and phosphoheptulonate synthase activity (GO:0003849) under control conditions, whereas genes representing peroxidase activity (GO:0004601), cinamoyl alcohol dehydrogenase (GO:0045551), anthranilate phosphoribosyl transferase (GO:0004048), indole-3-glycerol phosphate synthase (GO:0004425), heme (GO:0020037) and ferric ion binding (GO:0008199) were significantly enriched under stress in ewst1. The URDEGs for DNA binding (GO:0003677), Zinc ion binding (GO:0008270) and oxidoreductase (GO:0016702) were down-regulated in ewst1 under control. Interestingly, there was only one GO term, phosphoglycolate phosphatase (GO:0008967), that was significantly enriched among the down-regulated genes under stress. However, in cellular function GO enrichment analysis, GO terms like exocyst (GO:0000145), intracellular membrane bounded organelle (GO:0043231), membrane (GO:0016020) and extracellular region (GO:0005576) were found to be significant in the up-regulated gene set, whereas nucleus (GO:0005634) GO term was enriched in the down-regulated gene set of URDEGs in control conditions. The overview of GO enrichment analysis is depicted in Fig. 8. Pathway analysis of URDEGs indicated that there were significant expressional alterations in flavonoid biosynthesis, phenylpropanoid biosynthesis, starch and sucrose metabolism and tryptophan biosynthesis-related genes in ewst1 when compared with WT.Figure 8.

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus