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Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus

Comparative root study of ewst1 and the WT. (A) Development of root in a PVC tube under control and stress conditions. (B) Comparison of root traits like MRL, RW, RV and root number (RN) under control and stress conditions. (C) Relative values of maximum root length (RMRL), relative root weight (RRW), relative root volume (RRV) and relative total root number (RRN) under control and stress. (D) Anatomy of root magnifying the stele region (en, endodermis; cb, casparian band; pe, pericycle; cmx, central meta-xylem; mx, meta-xylem; ph, phloem; p, pith). Values are mean ± SE of three individual replications. Statistical significance determined using the Holm–Sidak method, with α = 5.0 %. Asterisks indicate significant differences between ewst1 and WT (Student's t-test: P < 0.05).
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PLV023F5: Comparative root study of ewst1 and the WT. (A) Development of root in a PVC tube under control and stress conditions. (B) Comparison of root traits like MRL, RW, RV and root number (RN) under control and stress conditions. (C) Relative values of maximum root length (RMRL), relative root weight (RRW), relative root volume (RRV) and relative total root number (RRN) under control and stress. (D) Anatomy of root magnifying the stele region (en, endodermis; cb, casparian band; pe, pericycle; cmx, central meta-xylem; mx, meta-xylem; ph, phloem; p, pith). Values are mean ± SE of three individual replications. Statistical significance determined using the Holm–Sidak method, with α = 5.0 %. Asterisks indicate significant differences between ewst1 and WT (Student's t-test: P < 0.05).

Mentions: The root analysis performed in samples taken from plants grown in PVC tubes revealed that there were significant differences in root growth parameters in ewst1 when compared with WT in response to water stress (Fig. 5A). There was a significant increase in RW, RV and RN of the mutant under well-watered conditions, and MRL and RV of the mutant under stress conditions when compared with its WT. Interestingly, no significant change was found in MRL under control, and RW and RN under stress conditions (Fig. 5B). However, in terms of relative value of root traits under control and stress conditions, the mutant showed a higher value for the relative maximum root length (RMRL) and lesser value for all other root parameters, namely RRW, RRV and relative root number on the crown when compared with the WT (Fig. 5C). Root anatomical studies revealed variations in the size and the number of cells in the stellar region between the mutant and the WT (Fig. 5D). The numbers of central meta-xylem, xylem and phloem cells were 5, 14 and 14 in WT, while these were 5, 9 and 9 in the mutant, respectively. The central meta-xylems were similar in shape and uniformly distributed in the WT, whereas they were of different shapes with reduced diameter and altered arrangement in the mutant.Figure 5.


Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Comparative root study of ewst1 and the WT. (A) Development of root in a PVC tube under control and stress conditions. (B) Comparison of root traits like MRL, RW, RV and root number (RN) under control and stress conditions. (C) Relative values of maximum root length (RMRL), relative root weight (RRW), relative root volume (RRV) and relative total root number (RRN) under control and stress. (D) Anatomy of root magnifying the stele region (en, endodermis; cb, casparian band; pe, pericycle; cmx, central meta-xylem; mx, meta-xylem; ph, phloem; p, pith). Values are mean ± SE of three individual replications. Statistical significance determined using the Holm–Sidak method, with α = 5.0 %. Asterisks indicate significant differences between ewst1 and WT (Student's t-test: P < 0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482838&req=5

PLV023F5: Comparative root study of ewst1 and the WT. (A) Development of root in a PVC tube under control and stress conditions. (B) Comparison of root traits like MRL, RW, RV and root number (RN) under control and stress conditions. (C) Relative values of maximum root length (RMRL), relative root weight (RRW), relative root volume (RRV) and relative total root number (RRN) under control and stress. (D) Anatomy of root magnifying the stele region (en, endodermis; cb, casparian band; pe, pericycle; cmx, central meta-xylem; mx, meta-xylem; ph, phloem; p, pith). Values are mean ± SE of three individual replications. Statistical significance determined using the Holm–Sidak method, with α = 5.0 %. Asterisks indicate significant differences between ewst1 and WT (Student's t-test: P < 0.05).
Mentions: The root analysis performed in samples taken from plants grown in PVC tubes revealed that there were significant differences in root growth parameters in ewst1 when compared with WT in response to water stress (Fig. 5A). There was a significant increase in RW, RV and RN of the mutant under well-watered conditions, and MRL and RV of the mutant under stress conditions when compared with its WT. Interestingly, no significant change was found in MRL under control, and RW and RN under stress conditions (Fig. 5B). However, in terms of relative value of root traits under control and stress conditions, the mutant showed a higher value for the relative maximum root length (RMRL) and lesser value for all other root parameters, namely RRW, RRV and relative root number on the crown when compared with the WT (Fig. 5C). Root anatomical studies revealed variations in the size and the number of cells in the stellar region between the mutant and the WT (Fig. 5D). The numbers of central meta-xylem, xylem and phloem cells were 5, 14 and 14 in WT, while these were 5, 9 and 9 in the mutant, respectively. The central meta-xylems were similar in shape and uniformly distributed in the WT, whereas they were of different shapes with reduced diameter and altered arrangement in the mutant.Figure 5.

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus