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Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus

Comparative stomatal behaviour of ewst1 and WT under control and stress conditions showing CO, PO and CC stomata.
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PLV023F4: Comparative stomatal behaviour of ewst1 and WT under control and stress conditions showing CO, PO and CC stomata.

Mentions: The measurement of various physiological parameters revealed that ewst1 showed an increased level of RWC, CMS and chlorophyll concentration under water deficit stress over the WT (Fig. 3D and E). Though the RWC of the mutant and WT showed no significant difference under control, upon water stress, the RWC of the mutant was found to be 11 % more than that of WT (Fig. 3D). On the basis of ionic leakage, the CMS of ewst1 was significantly higher (93.4 ± 4.3) when compared with the WT (78.3 ± 5.4) under stress. Similarly, the total chlorophyll concentration was significantly higher in ewst1 than the WT under stress without any significant change under control (Fig. 3E). The stomatal movement analyses under ESEM revealed that there were more PO stomata in the mutant under control, but more CC stomata and lesser CO stomata in ewst1 under stress conditions when compared with the WT (Fig. 4) [Supporting Information—Fig. S3].Figure 4.


Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Comparative stomatal behaviour of ewst1 and WT under control and stress conditions showing CO, PO and CC stomata.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482838&req=5

PLV023F4: Comparative stomatal behaviour of ewst1 and WT under control and stress conditions showing CO, PO and CC stomata.
Mentions: The measurement of various physiological parameters revealed that ewst1 showed an increased level of RWC, CMS and chlorophyll concentration under water deficit stress over the WT (Fig. 3D and E). Though the RWC of the mutant and WT showed no significant difference under control, upon water stress, the RWC of the mutant was found to be 11 % more than that of WT (Fig. 3D). On the basis of ionic leakage, the CMS of ewst1 was significantly higher (93.4 ± 4.3) when compared with the WT (78.3 ± 5.4) under stress. Similarly, the total chlorophyll concentration was significantly higher in ewst1 than the WT under stress without any significant change under control (Fig. 3E). The stomatal movement analyses under ESEM revealed that there were more PO stomata in the mutant under control, but more CC stomata and lesser CO stomata in ewst1 under stress conditions when compared with the WT (Fig. 4) [Supporting Information—Fig. S3].Figure 4.

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus