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Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus

Germination of ewst1 when compared with its WT Nagina22 (NC, Nagina22 control; NS, Nagina22 stress; MC, mutant control; MS, mutant stress). (A) Germination of ewst1 and Nagina22 in PEG-infused media under control and −0.7 MPa osmotic stress conditions. (B) Comparison of plumule development between WT and ewst1 under three different osmotic levels. (C) Comparison of radicle development between WT and ewst1 under three different osmotic levels. (B and C) Values are mean ± SE of three individual replications having 30 seeds in each plate. Statistical significance was determined using the Holm–Sidak method, with α = 5.000 %. Asterisks indicate significant differences between WT and ewst1 (Student's t-test P < 0.05).
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PLV023F2: Germination of ewst1 when compared with its WT Nagina22 (NC, Nagina22 control; NS, Nagina22 stress; MC, mutant control; MS, mutant stress). (A) Germination of ewst1 and Nagina22 in PEG-infused media under control and −0.7 MPa osmotic stress conditions. (B) Comparison of plumule development between WT and ewst1 under three different osmotic levels. (C) Comparison of radicle development between WT and ewst1 under three different osmotic levels. (B and C) Values are mean ± SE of three individual replications having 30 seeds in each plate. Statistical significance was determined using the Holm–Sidak method, with α = 5.000 %. Asterisks indicate significant differences between WT and ewst1 (Student's t-test P < 0.05).

Mentions: The mutant showed significant difference in radicle (ewst1 = 85.1 % and WT = 15.5 %) and plumule (ewst1 =65.1 % and WT = 95.5 %) growth at −0.7 MPa when compared with the WT, whereas there was no difference in control plates. At an osmotic potential of −0.5 MPa, there was a significant difference in radicle growth, whereas at −1.2 MPa plumule growth showed a significant difference. These results indicated that the ewst1 possessed greater germination percentage, and plumule and radicle development when compared with the WT under the water stress conditions (Fig. 2A–C).Figure 2.


Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Germination of ewst1 when compared with its WT Nagina22 (NC, Nagina22 control; NS, Nagina22 stress; MC, mutant control; MS, mutant stress). (A) Germination of ewst1 and Nagina22 in PEG-infused media under control and −0.7 MPa osmotic stress conditions. (B) Comparison of plumule development between WT and ewst1 under three different osmotic levels. (C) Comparison of radicle development between WT and ewst1 under three different osmotic levels. (B and C) Values are mean ± SE of three individual replications having 30 seeds in each plate. Statistical significance was determined using the Holm–Sidak method, with α = 5.000 %. Asterisks indicate significant differences between WT and ewst1 (Student's t-test P < 0.05).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482838&req=5

PLV023F2: Germination of ewst1 when compared with its WT Nagina22 (NC, Nagina22 control; NS, Nagina22 stress; MC, mutant control; MS, mutant stress). (A) Germination of ewst1 and Nagina22 in PEG-infused media under control and −0.7 MPa osmotic stress conditions. (B) Comparison of plumule development between WT and ewst1 under three different osmotic levels. (C) Comparison of radicle development between WT and ewst1 under three different osmotic levels. (B and C) Values are mean ± SE of three individual replications having 30 seeds in each plate. Statistical significance was determined using the Holm–Sidak method, with α = 5.000 %. Asterisks indicate significant differences between WT and ewst1 (Student's t-test P < 0.05).
Mentions: The mutant showed significant difference in radicle (ewst1 = 85.1 % and WT = 15.5 %) and plumule (ewst1 =65.1 % and WT = 95.5 %) growth at −0.7 MPa when compared with the WT, whereas there was no difference in control plates. At an osmotic potential of −0.5 MPa, there was a significant difference in radicle growth, whereas at −1.2 MPa plumule growth showed a significant difference. These results indicated that the ewst1 possessed greater germination percentage, and plumule and radicle development when compared with the WT under the water stress conditions (Fig. 2A–C).Figure 2.

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus